Abstract
We infected various nonflagellated mutants of Escherichia coli with fla-transducing phages and followed the kinetics of the appearance of motility. Our analysis revealed two distinct classes of region III fla genes. Class II fla genes (hag, flaD) functioned 15 min later than class I fla genes (flaN, flaB, flaC, flaO, flaA, flbD, flaQ, flaP) in flagellar morphogenesis. We suggest that the two classes of fla genes are involved in two different stages, initiation (class I) and completion (class II), of flagellar formation.
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Selected References
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- Iino T. Genetics of structure and function of bacterial flagella. Annu Rev Genet. 1977;11:161–182. doi: 10.1146/annurev.ge.11.120177.001113. [DOI] [PubMed] [Google Scholar]
- Komeda Y., Kutsukake K., Iino T. Definition of additional flagellar genes in Escherichia coli K12. Genetics. 1980 Feb;94(2):277–290. doi: 10.1093/genetics/94.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kondoh H. Isolation and characterization of nondefective transducing lambda bacteriophages carrying fla genes of Escherichia coli K-12. J Bacteriol. 1977 May;130(2):736–745. doi: 10.1128/jb.130.2.736-745.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kondoh H., Ozeki H. Deletion and amber mutants of fla loci in Escherichia coli K-12. Genetics. 1976 Nov;84(3):403–421. doi: 10.1093/genetics/84.3.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kondoh H. Tumbling chemotaxis mutants of Escherichia coli: possible gene-dependent effect of methionine starvation. J Bacteriol. 1980 May;142(2):527–534. doi: 10.1128/jb.142.2.527-534.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silverman M., Matsumura P., Simon M. The identification of the mot gene product with Escherichia coli-lambda hybrids. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3126–3130. doi: 10.1073/pnas.73.9.3126. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silverman M., Simon M. I. Bacterial flagella. Annu Rev Microbiol. 1977;31:397–419. doi: 10.1146/annurev.mi.31.100177.002145. [DOI] [PubMed] [Google Scholar]