Abstract
Mutations at the gyrB locus were found to decrease the degree of supercoiling of the Escherichia coli chromosome. The effect of a gyrB mutation on the repair of ultraviolet-induced deoxyribonucleic acid damage was studied by following the killing of strains of E. coli K-12 proficient and deficient in deoxyribonucleic acid repair. The effectiveness of both excision and postreplication types of deoxyribonucleic acid repair was found to be altered by this mutation, the former being apparently enhanced and the latter impaired.
Full text
PDF![18](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15f0/217046/21d2896a1439/jbacter00269-0028.png)
![19](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15f0/217046/c39fd3f368c7/jbacter00269-0029.png)
![20](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15f0/217046/10f4fdad0350/jbacter00269-0030.png)
![21](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15f0/217046/631d39961fb4/jbacter00269-0031.png)
![22](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15f0/217046/7a5cfb668485/jbacter00269-0032.png)
![23](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15f0/217046/6e64640d51c7/jbacter00269-0033.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donch J., Green M. H., Greenberg J. Interaction of the exr and lon genes in Escherichia coli. J Bacteriol. 1968 Nov;96(5):1704–1710. doi: 10.1128/jb.96.5.1704-1710.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fairweather N. F., Orr E., Holland I. B. Inhibition of deoxyribonucleic acid gyrase: effects on nucleic acid synthesis and cell division in Escherichia coli K-12. J Bacteriol. 1980 Apr;142(1):153–161. doi: 10.1128/jb.142.1.153-161.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gellert M., Mizuuchi K., O'Dea M. H., Nash H. A. DNA gyrase: an enzyme that introduces superhelical turns into DNA. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872–3876. doi: 10.1073/pnas.73.11.3872. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hansen F. G., von Meyenburg K. Characterization of the dnaA, gyrB and other genes in the dnaA region of the Escherichia coli chromosome on specialized transducing phages lambda tna. Mol Gen Genet. 1979 Sep;175(2):135–144. doi: 10.1007/BF00425529. [DOI] [PubMed] [Google Scholar]
- Hecht R. M., Stimpson D., Pettijohn D. Sedimentation properties of the bacterial chromosome as an isolated nucleoid and as an unfolded DNA fiber. Chromosomal DNA folding measured by rotor speed effects. J Mol Biol. 1977 Apr 15;111(3):257–277. doi: 10.1016/s0022-2836(77)80051-x. [DOI] [PubMed] [Google Scholar]
- Howard-Flanders P., Boyce R. P., Theriot L. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics. 1966 Jun;53(6):1119–1136. doi: 10.1093/genetics/53.6.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Howard-Flanders P., Theriot L. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics. 1966 Jun;53(6):1137–1150. doi: 10.1093/genetics/53.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orr E., Fairweather N. F., Holland I. B., Pritchard R. H. Isolation and characterisation of a strain carrying a conditional lethal mutation in the cou gene of Escherichia coli K12. Mol Gen Genet. 1979;177(1):103–112. doi: 10.1007/BF00267259. [DOI] [PubMed] [Google Scholar]
- Rupp W. D., Wilde C. E., 3rd, Reno D. L., Howard-Flanders P. Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli. J Mol Biol. 1971 Oct 14;61(1):25–44. doi: 10.1016/0022-2836(71)90204-x. [DOI] [PubMed] [Google Scholar]
- Shibata T., DasGupta C., Cunningham R. P., Radding C. M. Purified Escherichia coli recA protein catalyzes homologous pairing of superhelical DNA and single-stranded fragments. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1638–1642. doi: 10.1073/pnas.76.4.1638. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Snyder M., Drlica K. DNA gyrase on the bacterial chromosome: DNA cleavage induced by oxolinic acid. J Mol Biol. 1979 Jun 25;131(2):287–302. doi: 10.1016/0022-2836(79)90077-9. [DOI] [PubMed] [Google Scholar]
- Ulmer K. M., Gomez R. F., Sinskey A. J. Ionizing radiation damage to the folded chromosome of Escherichia coli K-12: sedimentation properties of irradiated nucleoids and chromosomal deoxyribonucleic acid. J Bacteriol. 1979 May;138(2):475–485. doi: 10.1128/jb.138.2.475-485.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Worcel A., Burgi E. On the structure of the folded chromosome of Escherichia coli. J Mol Biol. 1972 Nov 14;71(2):127–147. doi: 10.1016/0022-2836(72)90342-7. [DOI] [PubMed] [Google Scholar]
- von Wright A., Bridges B. A. Effect of tif expression, irradiation of recipient and presence of plasmid pKM101 on recovery of a marker from a donor exposed to ultraviolet light prior to conjugation. J Gen Microbiol. 1980 Aug;119(2):327–331. doi: 10.1099/00221287-119-2-327. [DOI] [PubMed] [Google Scholar]