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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Development. 2007 Dec;134(23):4297–4306. doi: 10.1242/dev.009282

Fig. 2. aPKC regulates subcellular localization of PAR1 in Xenopus ectoderm.

Fig. 2

(A,B) aPKC inhibits cortical localization of PAR1. (A) PAR1 is predominantly localized to the basolateral cortex of superficial ectoderm cells (arrows). (B) Overexpression of aPKC-CAAX mislocalizes PAR1 to the cytoplasm (arrowheads). (C,D) The apical localization of T560A, the non-phosphorylatable PAR1 mutant (C’) is not affected by aPKC-CAAX (D). At higher doses of injected RNA, T560A was distributed all around the cell cortex, both apically and basolaterally (C). The distribution of coinjected membrane-associated aPKC-CAAX is shown in B’ and D’, the increased apical staining is due to high amounts of endogenous aPKC detected by anti-aPKC antibody. B” and D”, merged images. Embryo injections were as described in Fig. 1. Frozen sections of stage 10.5-11 embryonic ectoderm were stained with anti-Myc (green) to detect PAR1 (B,D) and anti-aPKC antibodies (B’ and D’, red). At least 15 embryos per group were examined and representative sections of three independent experiments are shown.