Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Apr;146(1):141–148. doi: 10.1128/jb.146.1.141-148.1981

Tn2001, a transposon encoding chloramphenicol resistance in Pseudomonas aeruginosa.

S Iyobe, H Sagai, S Mitsuhashi
PMCID: PMC217063  PMID: 6260739

Abstract

We isolated a new transposon, Tn2001, from the group P-2 plasmid Rms159-1 in Pseudomonas aeruginosa. Tn2001-encoded chloramphenicol resistance did not result from the formation of chloramphenicol acetyltransferase. Tn2001 was transposable between temperate phages and conjugative and nonconjugative plasmids belonging to various incompatibility groups, including P-1, P-3, P-4, P-5, P-7, and P-8 in P. aeruginosa. Transposition occurred independently of the general recombination ability of the Pseudomonas host, and its frequency varied between 10(-1) and 10(-8), depending upon the donor and recipient replicons. Tn2001 transposition also occurred in a recombination-deficient strain of Escherichia coli. Agarose gel electrophoresis and electron microscopic observations revealed that Tn2001 could transpose to different sites in the RP4 replicon and that the transposed deoxyribonucleic acid fragment was 2.1 kilobases long.

Full text

PDF
141

Images in this article

144Image
on p.144
146Image
on p.146

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alton N. K., Vapnek D. Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9. Nature. 1979 Dec 20;282(5741):864–869. doi: 10.1038/282864a0. [DOI] [PubMed] [Google Scholar]
  2. Arber W., Iida S., Jütte H., Caspers P., Meyer J., Hänni C. Rearrangements of genetic material in Escherichia coli as observed on the bacteriophage P1 plasmid. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1197–1208. doi: 10.1101/sqb.1979.043.01.136. [DOI] [PubMed] [Google Scholar]
  3. Barth P. T., Grinter N. J., Bradley D. E. Conjugal transfer system of plasmid RP4: analysis by transposon 7 insertion. J Bacteriol. 1978 Jan;133(1):43–52. doi: 10.1128/jb.133.1.43-52.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barth P. T., Grinter N. J. Map of plasmid RP4 derived by insertion of transposon C. J Mol Biol. 1977 Jul 5;113(3):455–474. doi: 10.1016/0022-2836(77)90233-9. [DOI] [PubMed] [Google Scholar]
  5. Beck E., Sommer R., Auerswald E. A., Kurz C., Zink B., Osterburg G., Schaller H., Sugimoto K., Sugisaki H., Okamoto T. Nucleotide sequence of bacteriophage fd DNA. Nucleic Acids Res. 1978 Dec;5(12):4495–4503. doi: 10.1093/nar/5.12.4495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bryan L. E., Semaka S. D., Van den Elzen H. M., Kinnear J. E., Whitehouse R. L. Characteristics of R931 and other Pseudomonas aeruginosa R factors. Antimicrob Agents Chemother. 1973 May;3(5):625–637. doi: 10.1128/aac.3.5.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bryan L. E., Shahrabadi M. S., van den Elzen H. M. Gentamicin resistance in Pseudomonas aeruginosa: R-factor-mediated resistance. Antimicrob Agents Chemother. 1974 Aug;6(2):191–199. doi: 10.1128/aac.6.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bryan L. E., Van Den Elzen H. M., Tseng J. T. Transferable drug resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1972 Jan;1(1):22–29. doi: 10.1128/aac.1.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dean H. F., Morgan A. F., Asche L. V., Holloway B. W. Isolates of Pseudomonas aeruginosa from Australian hospitals having R-plasmid determined antibiotic resistance. Med J Aust. 1977 Jul 23;2(4):116–119. doi: 10.5694/j.1326-5377.1977.tb99084.x. [DOI] [PubMed] [Google Scholar]
  12. Gottesman M. M., Rosner J. L. Acquisition of a determinant for chloramphenicol resistance by coliphage lambda. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5041–5045. doi: 10.1073/pnas.72.12.5041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HOLLOWAY B. W., MONK M. Transduction in Pseudomonas aeruginosa. Nature. 1959 Oct 31;184(Suppl 18):1426–1427. doi: 10.1038/1841426b0. [DOI] [PubMed] [Google Scholar]
  14. Hedges R. W., Jacob A. E. Transposition of ampicillin resistance from RP4 to other replicons. Mol Gen Genet. 1974;132(1):31–40. doi: 10.1007/BF00268228. [DOI] [PubMed] [Google Scholar]
  15. Howard-Flanders P., Theriot L. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics. 1966 Jun;53(6):1137–1150. doi: 10.1093/genetics/53.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Iida S., Arber W. Plaque forming specialized transducing phage P1: isolation of P1CmSmSu, a precursor of P1Cm. Mol Gen Genet. 1977 Jun 24;153(3):259–269. doi: 10.1007/BF00431591. [DOI] [PubMed] [Google Scholar]
  17. Ike Y., Hashimoto H., Motohashi K., Fujisawa N., Mitsuhashi S. Isolation and characterization of a composite plasmid Rms201 mutant temperature sensitive for replication. J Bacteriol. 1980 Feb;141(2):577–583. doi: 10.1128/jb.141.2.577-583.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Iyobe S., Hasuda K., Fuse A., Mitsuhashi S. Demonstration of R factors from Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1974 Jun;5(6):547–552. doi: 10.1128/aac.5.6.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. KONDO E., MITSUHASHI S. DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1. J Bacteriol. 1964 Nov;88:1266–1276. doi: 10.1128/jb.88.5.1266-1276.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kupersztoch-Portnoy Y. M., Lovett M. A., Helinski D. R. Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6K. Biochemistry. 1974 Dec 31;13(27):5484–5490. doi: 10.1021/bi00724a005. [DOI] [PubMed] [Google Scholar]
  21. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  22. Marcoli R., Iida S., Bickle T. A. The DNA sequence of an IS/-flanked transposon coding for resistance to chloramphenicol and fusidic acid. FEBS Lett. 1980 Jan 28;110(1):11–14. doi: 10.1016/0014-5793(80)80011-1. [DOI] [PubMed] [Google Scholar]
  23. Novick R. P., Edelman I., Schwesinger M. D., Gruss A. D., Swanson E. C., Pattee P. A. Genetic translocation in Staphylococcus aureus. Proc Natl Acad Sci U S A. 1979 Jan;76(1):400–404. doi: 10.1073/pnas.76.1.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rubens C. E., McNeill W. F., Farrar W. E., Jr Transposable plasmid deoxyribonucleic acid sequence in Pseudomonas aeruginosa which mediates resistance to gentamicin and four other antimicrobial agents. J Bacteriol. 1979 Sep;139(3):877–882. doi: 10.1128/jb.139.3.877-882.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sagai H., Hasuda K., Iyobe S., Bryan L. E., Holloway B. W., Mitsuhashi S. Classification of R plasmids by incompatibility in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1976 Oct;10(4):573–578. doi: 10.1128/aac.10.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sagai H., Uyobe S., Mitsuhashi S. Inhibition and facilitation of transfer among Pseudomonas aeruginos R plasmids. J Bacteriol. 1977 Sep;131(3):765–769. doi: 10.1128/jb.131.3.765-769.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sharp P. A., Hsu M. T., Otsubo E., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972 Nov 14;71(2):471–497. doi: 10.1016/0022-2836(72)90363-4. [DOI] [PubMed] [Google Scholar]
  28. So M., Heffron F., McCarthy B. J. The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1. Nature. 1979 Feb 8;277(5696):453–456. doi: 10.1038/277453a0. [DOI] [PubMed] [Google Scholar]
  29. Stanisich V. A., Bennett P. M., Richmond M. H. Characterization of a translocation unit encoding resistance to mercuric ions that occurs on a nonconjugative plasmid in Pseudomonas aeruginosa. J Bacteriol. 1977 Mar;129(3):1227–1233. doi: 10.1128/jb.129.3.1227-1233.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yamagishi H., Inokuchi H., Ozeki H. Excision and duplication of su3+-transducing fragments carried by bacteriophage phi 80. I. Novel structure of phi 80sus2psu3+ DNA molecule. J Virol. 1976 Jun;18(3):1016–1023. doi: 10.1128/jvi.18.3.1016-1023.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES