Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Apr;146(1):149–154. doi: 10.1128/jb.146.1.149-154.1981

Regulation of galactose operon expression: glucose effects and role of cyclic adenosine 3',5'-monophosphate.

E Joseph, A Danchin, A Ullmann
PMCID: PMC217064  PMID: 6260740

Abstract

We studied the following two aspects of the glucose effect on galactose operon expression in Escherichia coli K-12: catabolite repression and inducer exclusion. Using both inducible and constitutive strains and measuring the rate of promoter-proximal enzyme synthesis, we found that the galactose operon did not seem to exhibit catabolite repression. The only glucose effect on galactose operon expression which we observed was inducer exclusion, as shown by the existence of diauxic growth in the presence of glucose and galactose. This diauxie was not relieved by cyclic adenosine 3',5'-monophosphate. Cyclic adenosine 3',5'-monophosphate did not seem to be an antagonist of any glucose effect on galactose operon expression; its only effect was to stimulate promoter-distal gene expression.

Full text

PDF
149

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adhya S., Echols H. Glucose effect and the galactose enzymes of Escherichia coli: correlation between glucose inhibition of induction and inducer transport. J Bacteriol. 1966 Sep;92(3):601–608. doi: 10.1128/jb.92.3.601-608.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adhya S., Miller W. Modulation of the two promoters of the galactose operon of Escherichia coli. Nature. 1979 Jun 7;279(5713):492–494. doi: 10.1038/279492a0. [DOI] [PubMed] [Google Scholar]
  3. BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. I. LA BIOSYNTH'ESE INDUITE DE LA GALACTOKINASE ET L'INDUCTION SIMULTAN'EE DE LA S'EQUENCE ENZYMATIQUE. J Mol Biol. 1963 Aug;7:164–182. doi: 10.1016/s0022-2836(63)80044-3. [DOI] [PubMed] [Google Scholar]
  4. Danchin A., Dondon L. Serine sensitivity of Escherichia coli K 12: partial characterization of a serine resistnat mutant that is extremely sensitive to 2-ketobutyrate. Mol Gen Genet. 1980 Apr;178(1):155–164. doi: 10.1007/BF00267224. [DOI] [PubMed] [Google Scholar]
  5. Fiethen L., Starlinger P. Mutations in the galactose-operator. Mol Gen Genet. 1970;108(4):322–330. doi: 10.1007/BF00267769. [DOI] [PubMed] [Google Scholar]
  6. Guidi-Rontani C., Danchin A., Ullmann A. Catabolite repression in Escherichia coli mutants lacking cyclic AMP receptor protein. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5799–5801. doi: 10.1073/pnas.77.10.5799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HORECKER B. L., THOMAS J., MONOD J. Galactose transport in Escherichia coli. II. Characteristics of the exit process. J Biol Chem. 1960 Jun;235:1586–1590. [PubMed] [Google Scholar]
  8. Hua S. S., Markovitz A. Multiple regulation of the galactose operon-genetic evidence for a distinct site in the galactose operon that responds to capR gene regulation in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1974 Feb;71(2):507–511. doi: 10.1073/pnas.71.2.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hua S. S., Markovitz A. Multiple regulator gene control of the galactose operon in Escherichia coli K-12. J Bacteriol. 1972 Jun;110(3):1089–1099. doi: 10.1128/jb.110.3.1089-1099.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MAGASANIK B. Catabolite repression. Cold Spring Harb Symp Quant Biol. 1961;26:249–256. doi: 10.1101/sqb.1961.026.01.031. [DOI] [PubMed] [Google Scholar]
  11. Musso R. E., Di Lauro R., Adhya S., de Crombrugghe B. Dual control for transcription of the galactose operon by cyclic AMP and its receptor protein at two interspersed promoters. Cell. 1977 Nov;12(3):847–854. doi: 10.1016/0092-8674(77)90283-5. [DOI] [PubMed] [Google Scholar]
  12. Petit-Koskas E., Contesse G. Stimulation in trans of synthesis of E. coli gal operon enzymes by lambdoid phages during low catabolite repression. Mol Gen Genet. 1976 Jan 16;143(2):203–209. doi: 10.1007/BF00266923. [DOI] [PubMed] [Google Scholar]
  13. Taniguchi T., O'Neill M., de Crombrugghe B. Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5090–5094. doi: 10.1073/pnas.76.10.5090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ullmann A., Joseph E., Danchin A. Cyclic AMP as a modulator of polarity in polycistronic transcriptional units. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3194–3197. doi: 10.1073/pnas.76.7.3194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wanner B. L., Kodaira R., Neidhardt F. C. Regulation of lac operon expression: reappraisal of the theory of catabolite repression. J Bacteriol. 1978 Dec;136(3):947–954. doi: 10.1128/jb.136.3.947-954.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wetekam W., Staack K., Ehring R. DNA-dependent in vitro synthesis of enzymes of the galactose operon of Escherichia coli. Mol Gen Genet. 1971;112(1):14–27. doi: 10.1007/BF00266928. [DOI] [PubMed] [Google Scholar]
  17. Wetekam W., Staack K., Ehring R. Relief of polarity in DNA-dependent cell-free synthesis of enzymes of the galactose operon of Escherichia coli. Mol Gen Genet. 1972;116(3):258–276. doi: 10.1007/BF00269770. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES