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. 1981 Apr;146(1):285–290. doi: 10.1128/jb.146.1.285-290.1981

Postreplication repair in Saccharomyces cerevisiae.

M A Resnick, J Boyce, B Cox
PMCID: PMC217080  PMID: 7012117

Abstract

Postreplication events in logarithmically growing excision-defective mutants of Saccharomyces cerevisiae were examined after low doses of ultraviolet light (2 to 4 J/m2). Pulse-labeled deoxyribonucleic acid had interruptions, and when the cells were "chased," the interruptions were no longer detected. Since the loss of interruptions was not associated with an exchange of pyrimidine dimers at a detection level of 10 to 20% of the induced dimers, we concluded that postreplication repair in excision-defective mutants (or leaky mutants) does not involve molecular recombination. Pyrimidine dimers were assayed by utilizing the ultraviolet-endonuclease activity in extracts of Micrococcus luteus and newly developed alkaline sucrose gradient techniques, which yielded chromosomal-size deoxyribonucleic acid after treatment of irradiated cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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