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. 1981 Apr;146(1):337–344. doi: 10.1128/jb.146.1.337-344.1981

Induction of citric acid cycle enzymes during initiation of sporulation by guanine nucleotide deprivation.

B Uratani-Wong, J M Lopez, E Freese
PMCID: PMC217088  PMID: 6783618

Abstract

In Bacillus subtilis, conditions causing partial deprivation of guanine nucleotides initiated sporulation and caused the synthesis of citrate synthase, aconitase, and alpha-ketoglutarate dehydrogenase. Alpha-ketoglutarate dehydrogenase could also be induced by acetate, and the specific activity of this enzyme was elevated in mutants that had high intracellular acetyl coenzyme A concentrations because they lacked citrate synthase activity. After deprivation of guanine nucleotides, the intracellular concentration of acetyl coenzyme A also increased, which explained the induction of alpha-ketoglutarate dehydrogenase. Furthermore, the decreases in alpha-ketoglutarate and L-malate concentrations observed during this deprivation accounted for the observed increases in citrate synthase activity (which was repressed by alpha-ketoglutarate and malate) and aconitase activity (which was repressed by alpha-ketoglutarate).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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