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. 1981 Apr;146(1):422–425. doi: 10.1128/jb.146.1.422-425.1981

Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli.

S Brown, E R Brickman, J Beckwith
PMCID: PMC217102  PMID: 7012125

Abstract

We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.

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Selected References

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