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. 1981 Mar;145(3):1222–1231. doi: 10.1128/jb.145.3.1222-1231.1981

Attachment of the main chain to the linkage unit in biosynthesis of teichoic acids.

H A McArthur, I C Hancock, J Baddiley
PMCID: PMC217122  PMID: 6782090

Abstract

The main chain of teichoic acids can be assembled in cell-free membrane preparations by the transfer of residues from the appropriate nucleotide precursors to an incompletely characterized amphiphilic molecule, lipoteichoic acid carrier (LTC). However, in the cell wall, the main chain is attached to peptidoglycan through a linkage unit which is synthesized independently. It is believed that, in these cell-free systems, lipid intermediates carrying linkage units are also able to accept residues directly from nucleotide precursors to build up the main chain. In this paper, we have shown that the main chain attached to LTC was transferred from LTC to lipids containing the linkage unit. Thus, in these systems, there appear to be two routes to the biosynthesis of teichoic acid-linkage unit complexes, one by direct assembly of the main chain on linkage unit lipids and the other by transfer of the preassembled main chain from LTC to the linkage unit. It was also shown that linkage unit lipids from different organisms were interchangeable and that these were used for polymer synthesis by Bacillus subtilis 3610, in which the teichoic acid is a poly(glycerol phosphate).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. W., Baum P. R., Gesteland R. F. Processing of adenovirus 2-induced proteins. J Virol. 1973 Aug;12(2):241–252. doi: 10.1128/jvi.12.2.241-252.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson R. G., Hussey H., Baddiley J. The mechanism of wall synthesis in bacteria. The organization of enzymes and isoprenoid phosphates in the membrane. Biochem J. 1972 Mar;127(1):11–25. doi: 10.1042/bj1270011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baddiley J., Blumsom N. L., Douglas L. J. The biosynthesis of the wall teichoic acid in Staphylococcus lactis I3. Biochem J. 1968 Dec;110(3):565–571. doi: 10.1042/bj1100565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bracha R., Chang M., Fiedler F., Glaser L. Biosynthesis of teichoic acids. Methods Enzymol. 1978;50:387–340. doi: 10.1016/0076-6879(78)50046-3. [DOI] [PubMed] [Google Scholar]
  5. Bracha R., Davidson R., Mirelman D. Defect in biosynthesis of the linkage unit between peptidoglycan and teichoic acid in a bacteriophage-resistant mutant of Staphylococcus aureus. J Bacteriol. 1978 May;134(2):412–417. doi: 10.1128/jb.134.2.412-417.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bracha R., Glaser L. An intermediate in telchoic acid biosynthesis. Biochem Biophys Res Commun. 1976 Oct 4;72(3):1091–1098. doi: 10.1016/s0006-291x(76)80244-6. [DOI] [PubMed] [Google Scholar]
  7. Bracha R., Glaser L. In vitro system for the synthesis of teichoic acid linked to peptidoglycan. J Bacteriol. 1976 Mar;125(3):872–879. doi: 10.1128/jb.125.3.872-879.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Brooks D., Baddiley J. A lipid intermediate in the synthesis of a poly-(N-acetylglucosamine 1-phosphate) from the wall of Staphylococcus lactis N.C.T.C. 2102. Biochem J. 1969 Nov;115(2):307–314. doi: 10.1042/bj1150307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Brooks D., Baddiley J. The mechanism of biosynthesis and direction of chain extension of a poly-(N-acetylglucosamine 1-phosphate) from the walls of Staphylococcus lactis N.C.T.C. 2102. Biochem J. 1969 Jul;113(4):635–642. doi: 10.1042/bj1130635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Childs W. C., 3rd, Neuhaus F. C. Biosynthesis of D-alanyl-lipoteichoic acid: characterization of ester-linked D-alanine in the in vitro-synthesized product. J Bacteriol. 1980 Jul;143(1):293–301. doi: 10.1128/jb.143.1.293-301.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Coley J., Tarelli E., Archibald A. R., Baddiley J. The linkage between teichoic acid and peptidoglycan in bacterial cell walls. FEBS Lett. 1978 Apr 1;88(1):1–9. doi: 10.1016/0014-5793(78)80594-8. [DOI] [PubMed] [Google Scholar]
  12. Douglas L. J., Baddiley J. A lipid intermediate in the biosynthesis of a teichoic acid. FEBS Lett. 1968 Aug;1(2):114–116. doi: 10.1016/0014-5793(68)80034-1. [DOI] [PubMed] [Google Scholar]
  13. Fiedler F., Glaser L. The synthesis of polyribitol phosphate. I. Purification of polyribitol phosphate polymerase and lipoteichoic acid carrier. J Biol Chem. 1974 May 10;249(9):2684–2689. [PubMed] [Google Scholar]
  14. Fiedler F., Glaser L. The synthesis of polyribitol phosphate. II. On the mechanism of polyribitol phosphate polymerase. J Biol Chem. 1974 May 10;249(9):2690–2695. [PubMed] [Google Scholar]
  15. Fischer W., Koch H. U., Rösel P., Fiedler F. Alanine ester-containing native lipoteichoic acids do not act as lipoteichoic acid carrier. Isolation, structural and functional characterization. J Biol Chem. 1980 May 25;255(10):4557–4562. [PubMed] [Google Scholar]
  16. Fischer W., Koch H. U., Rösel P., Fiedler F., Schmuck L. Structural requirements of lipoteichoic acid carrier for recognition by the poly(ribitol phosphate) polymerase from Staphylococcus aureus H. A study of various lipoteichoic acids, derivatives, and related compounds. J Biol Chem. 1980 May 25;255(10):4550–4556. [PubMed] [Google Scholar]
  17. GHUYSEN J. M., TIPPER D. J., STROMINGER J. L. STRUCTURE OF THE CELL WALL OF STAPHYLOCOCCUS AUREUS, STRAIN COPENHAGEN. IV. THE TEICHOIC ACID-GLYCOPEPTIDE COMPLEX. Biochemistry. 1965 Mar;4:474–485. doi: 10.1021/bi00879a016. [DOI] [PubMed] [Google Scholar]
  18. Hancock I. C., Wiseman G., Baddiley J. Biosynthesis of the unit that links teichoic acid to the bacterial wall: inhibition by tunicamycin. FEBS Lett. 1976 Oct 15;69(1):75–80. doi: 10.1016/0014-5793(76)80657-6. [DOI] [PubMed] [Google Scholar]
  19. Hancock I., Baddiley J. In vitro synthesis of the unit that links teichoic acid to peptidoglycan. J Bacteriol. 1976 Mar;125(3):880–886. doi: 10.1128/jb.125.3.880-886.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Higashi Y., Siewert G., Strominger J. L. Biosynthesis of the peptidoglycan of bacterial cell walls. XIX. Isoprenoid alcohol phosphokinase. J Biol Chem. 1970 Jul 25;245(14):3683–3690. [PubMed] [Google Scholar]
  21. Hussey H., Baddiley J. Lipid intermediates in the biosynthesis of the wall teichoic acid in Staphylococcus lactis 13. Biochem J. 1972 Mar;127(1):39–50. doi: 10.1042/bj1270039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  23. Mauck J., Glaser L. An acceptor-dependent polyglycerolphosphate polymerase. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2386–2390. doi: 10.1073/pnas.69.9.2386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. McArthur H. A., Hancock I. C., Roberts F. M., Baddiley J. Biosynthesis of teichoic acid in Micrococcus varians ATCC 29750. Characterization of a further lipid intermediate. FEBS Lett. 1980 Mar 10;111(2):317–323. doi: 10.1016/0014-5793(80)80818-0. [DOI] [PubMed] [Google Scholar]
  25. McArthur H. A., Roberts F. M., Hancock I. C., Baddiley J. Lipid intermediates in the biosynthesis of the linkage unit between teichoic acids and peptidoglycan. FEBS Lett. 1978 Feb 15;86(2):193–200. doi: 10.1016/0014-5793(78)80561-4. [DOI] [PubMed] [Google Scholar]
  26. Osborn M. J. Structure and biosynthesis of the bacterial cell wall. Annu Rev Biochem. 1969;38:501–538. doi: 10.1146/annurev.bi.38.070169.002441. [DOI] [PubMed] [Google Scholar]
  27. Roberts F. M., McArthur H. A., Hancock I. C., Baddiley J. Biosynthesis of the linkage unit joining peptidoglycan to poly(N-acetylglucosamine 1-phosphate) in walls of Micrococcus varians ATCC 29750. FEBS Lett. 1979 Jan 15;97(2):211–216. doi: 10.1016/0014-5793(79)80086-1. [DOI] [PubMed] [Google Scholar]
  28. Tonn S. J., Gander J. E. Biosynthesis of polysaccharides by prokaryotes. Annu Rev Microbiol. 1979;33:169–199. doi: 10.1146/annurev.mi.33.100179.001125. [DOI] [PubMed] [Google Scholar]
  29. Troy F. A., Frerman F. E., Heath E. C. The biosynthesis of capsular polysaccharide in Aerobacter aerogenes. J Biol Chem. 1971 Jan 10;246(1):118–133. [PubMed] [Google Scholar]
  30. Watkinson R. J., Hussey H., Baddiley J. Shared lipid phosphate carrier in the biosynthesis of teichoic acid and peptidoglycan. Nat New Biol. 1971 Jan 13;229(2):57–59. doi: 10.1038/newbio229057a0. [DOI] [PubMed] [Google Scholar]
  31. Wyke A. W., Ward J. B. Biosynthesis of wall polymers in Bacillus subtilis. J Bacteriol. 1977 Jun;130(3):1055–1063. doi: 10.1128/jb.130.3.1055-1063.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wyke A. W., Ward J. B. The biosynthesis of muramic acid phosphate in Bacillus licheniformis. FEBS Lett. 1977 Feb 1;73(2):159–163. doi: 10.1016/0014-5793(77)80971-x. [DOI] [PubMed] [Google Scholar]
  33. Wyke A. W., Ward J. B. The synthesis of covalently-linked teichoic acid and peptidoglycan by cell-free preparations of Bacillus licheniformis. Biochem Biophys Res Commun. 1975 Aug 4;65(3):877–885. doi: 10.1016/s0006-291x(75)80467-0. [DOI] [PubMed] [Google Scholar]

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