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. 1981 Mar;145(3):1305–1309. doi: 10.1128/jb.145.3.1305-1309.1981

Homology between Escherichia coli plasmids ColE1 and p15A.

R E Bird
PMCID: PMC217133  PMID: 6259130

Abstract

The location and extent of the homology between plasmids ColE1 and p15A were determined by analysis of heteroduplexes formed between them as well as with a related plasmid, pBR322, and by hybridization of radioactive deoxyribonucleic acids to restriction fragments of p15A and ColE1. The homology between the plasmids contained the entire region of ColE1 required for its replication as well as an additional 400 base pairs downstream from the origin of replication. This region on p15A, which was 980 +/- 43 base pairs, started at 0.1 of the molecular length from one end formed by cleavage with the restriction endonuclease BglI and extended to 0.54 of the molecular length from the same end. Restriction cleavage maps for the enzymes BglI, HpaI, HaeII, HaeIII, and HincII are also presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bastia D. The nucleotide sequence surrounding the origin of DNA replication of Col E1. Nucleic Acids Res. 1977 Sep;4(9):3123–3142. doi: 10.1093/nar/4.9.3123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bolivar F., Betlach M. C., Heyneker H. L., Shine J., Rodriguez R. L., Boyer H. W. Origin of replication of pBR345 plasmid DNA. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5265–5269. doi: 10.1073/pnas.74.12.5265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  4. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clewell D. B., Evenchik B., Cranston J. W. Direct inhibition of Col E 1 plasmid DNA replication in Escherichia coli by rifampicin. Nat New Biol. 1972 May 3;237(70):29–31. doi: 10.1038/newbio237029a0. [DOI] [PubMed] [Google Scholar]
  6. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cozzarelli N. R., Kelly R. B., Kornberg A. A minute circular DNA from Escherichia coli 15. Proc Natl Acad Sci U S A. 1968 Jul;60(3):992–999. doi: 10.1073/pnas.60.3.992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goebel W., Schrempf H. Isolation of minicircular deoxyribonucleic acids from wild strains of Escherichia coli and their relationship to other bacterial plasmids. J Bacteriol. 1972 Sep;111(3):696–704. doi: 10.1128/jb.111.3.696-704.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hashimoto-Gotoh T., Inselburg J. ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility. J Bacteriol. 1979 Aug;139(2):608–619. doi: 10.1128/jb.139.2.608-619.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Inselburg J. Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256–2259. doi: 10.1073/pnas.71.6.2256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lovett M. A., Katz L., Helinski D. R. Unidirectional replication of plasmid ColE1 DNA. Nature. 1974 Sep 27;251(5473):337–340. doi: 10.1038/251337a0. [DOI] [PubMed] [Google Scholar]
  12. Messing J., Staudenbauer W. L., Hofschneider P. H. Inhibition of minicircular DNA replication in Escherichia coli 15 by rifampicin. Nat New Biol. 1972 Aug 16;238(85):202–203. doi: 10.1038/newbio238202a0. [DOI] [PubMed] [Google Scholar]
  13. Ohmori H., Tomizawa J. Nucleotide sequence of the region required for maintenance of colicin E1 plasmid. Mol Gen Genet. 1979 Oct 3;176(2):161–170. doi: 10.1007/BF00273210. [DOI] [PubMed] [Google Scholar]
  14. Oka A., Takanami M. Cleavage map of colicin E1 plasmid. Nature. 1976 Nov 11;264(5582):193–196. doi: 10.1038/264193a0. [DOI] [PubMed] [Google Scholar]
  15. Roberts R. J., Breitmeyer J. B., Tabachnik N. F., Myers P. A. A second specific endonuclease from Haemophilus aegyptius. J Mol Biol. 1975 Jan 5;91(1):121–123. doi: 10.1016/0022-2836(75)90375-7. [DOI] [PubMed] [Google Scholar]
  16. Sakakibara Y., Tomizawa J. Replication of colicin E1 plasmid DNA in cell extracts. II. Selective synthesis of early replicative intermediates. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1403–1407. doi: 10.1073/pnas.71.4.1403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shepard H. M., Gelfand D. H., Polisky B. Analysis of a recessive plasmid copy number mutant: evidence for negative control of Col E1 replication. Cell. 1979 Oct;18(2):267–275. doi: 10.1016/0092-8674(79)90046-1. [DOI] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  20. Tomizawa J. I., Ohmori H., Bird R. E. Origin of replication of colicin E1 plasmid DNA. Proc Natl Acad Sci U S A. 1977 May;74(5):1865–1869. doi: 10.1073/pnas.74.5.1865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Twigg A. J., Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature. 1980 Jan 10;283(5743):216–218. doi: 10.1038/283216a0. [DOI] [PubMed] [Google Scholar]

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