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. 1981 Mar;145(3):1310–1316. doi: 10.1128/jb.145.3.1310-1316.1981

Functional organization of plasmid pKM101.

P J Langer, W G Shanabruch, G C Walker
PMCID: PMC217134  PMID: 6259131

Abstract

Tn5 insertion mutants and in vitro-generated deletion mutants of the mutagenesis-enhancing plasmid pKM101 have been used to identify several genetic regions on the pKM101 map. In clockwise order on the pKM101 map are: (i) the bla gene, coding for a beta-lactamase; (ii) the Slo region, responsible for retarding cell growth on minimal medium; (iii) the tra genes, enabling pKM101 to transfer conjugally; (iv) sensitivity to IKe phage (this function[s] maps within the tra region); (v) the muc gene(s), responsible for enhancing ultraviolet light and chemically induced mutagenesis in the cell; and (vi) the Rep region, essential for plasmid replication. The muc gene(s) and the Rep region are contained in a deoxyribonucleic acid region bounded by inverted repeated sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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