Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Mar;145(3):1413–1416. doi: 10.1128/jb.145.3.1413-1416.1981

Deoxyribonucleic acid replication in permeable and fully viable Escherichia coli cells.

E Boye, S Alver, K Skarstad
PMCID: PMC217147  PMID: 7009586

Abstract

Escherichia coli cells made permeable with a hypotonic tris(hydroxymethyl)aminomethane buffer utilized exogenous deoxyribonucleoside triphosphates to perform semiconservative replication. The rate of replication was the same as in cells made permeable with toluene or sucrose.

Full text

PDF
1413

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ananthaswamy H. N. The release of endonuclease I from Escherichia coli by a new cold shock procedure. Biochem Biophys Res Commun. 1976 May 23;76(2):289–298. doi: 10.1016/0006-291x(77)90724-0. [DOI] [PubMed] [Google Scholar]
  2. Atherly A. G. Ribonucleic acid regulation in premeabilized cells of Escherichia coli capable of ribonucleic acid and protein synthesis. J Bacteriol. 1974 Jun;118(3):1186–1189. doi: 10.1128/jb.118.3.1186-1189.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barbour S. D., Clark A. J. Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes. Proc Natl Acad Sci U S A. 1970 Apr;65(4):955–961. doi: 10.1073/pnas.65.4.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boye E. DNA repair and replication in cells of Escherichia coli made permeable with hypotonic buffers. Radiat Res. 1980 Oct;84(1):35–45. [PubMed] [Google Scholar]
  6. Boye E., Krisch R. E. Induction and repair of double- and single-strand DNA breaks in bacteriophage lambda superinfecting Escherichia coli. Int J Radiat Biol Relat Stud Phys Chem Med. 1980 Feb;37(2):119–133. doi: 10.1080/09553008014550191. [DOI] [PubMed] [Google Scholar]
  7. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  8. Gellert M., O'Dea M. H., Itoh T., Tomizawa J. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4474–4478. doi: 10.1073/pnas.73.12.4474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kornberg A. Aspects of DNA replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):1–9. doi: 10.1101/sqb.1979.043.01.003. [DOI] [PubMed] [Google Scholar]
  10. Kubitschek H. E., Freedman M. L. Chromosome replication and the division cycle of Escherichia coli B-r. J Bacteriol. 1971 Jul;107(1):95–99. doi: 10.1128/jb.107.1.95-99.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lazzarini R. A., Johnson L. D. Regulation of ribosomal RNA synthesis in cold-shocked E. coli. Nat New Biol. 1973 May 2;243(122):17–20. [PubMed] [Google Scholar]
  12. Mizuuchi K., O'Dea M. H., Gellert M. DNA gyrase: subunit structure and ATPase activity of the purified enzyme. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5960–5963. doi: 10.1073/pnas.75.12.5960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moses R. E., Richardson C. C. Replication and repair of DNA in cells of Escherichia coli treated with toluene. Proc Natl Acad Sci U S A. 1970 Oct;67(2):674–681. doi: 10.1073/pnas.67.2.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Neu H. C., Heppel L. A. On the surface localization of enzymes in E. coli. Biochem Biophys Res Commun. 1964 Oct 14;17(3):215–219. doi: 10.1016/0006-291x(64)90386-9. [DOI] [PubMed] [Google Scholar]
  15. Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
  16. Raué H. A., Cashel M. Regulation of RNA synthesis in Escherichia coli. I. Characterization of cells subjected to simultaneous temperature and osmotic shock. Biochim Biophys Acta. 1973 Jul 27;312(4):722–736. doi: 10.1016/0005-2787(73)90076-2. [DOI] [PubMed] [Google Scholar]
  17. Ryan M. J. Coumermycin A1: A preferential inhibitor of replicative DNA synthesis in Escherichia coli. I. In vivo characterization. Biochemistry. 1976 Aug 24;15(17):3769–3777. doi: 10.1021/bi00662a020. [DOI] [PubMed] [Google Scholar]
  18. Ryan M. J., Wells R. D. Coumerimycin A1: A preferential inhibitor of replicative DNA synthesis in Escherichia coli. II. In vivo characterization. Biochemistry. 1976 Aug 24;15(17):3778–3782. doi: 10.1021/bi00662a021. [DOI] [PubMed] [Google Scholar]
  19. Smeaton J. R., Elliott W. H. Selective release of ribonuclease-inhibitor from Bacillus subtilis cells by cold shock treatment. Biochem Biophys Res Commun. 1967 Jan 10;26(1):75–81. doi: 10.1016/0006-291x(67)90255-0. [DOI] [PubMed] [Google Scholar]
  20. Smith D. H., Davis B. D. Mode of action of novobiocin in Escherichia coli. J Bacteriol. 1967 Jan;93(1):71–79. doi: 10.1128/jb.93.1.71-79.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Staudenbauer W. L. Letters to the editor: Novobiocin-a specific inhibitor of semiconservative DNA replication in permeabilized Escherichia coli cells. J Mol Biol. 1975 Jul 25;96(1):201–205. doi: 10.1016/0022-2836(75)90191-6. [DOI] [PubMed] [Google Scholar]
  22. Staudenbauer W. L. Replication of small plasmids in extracts of Escherichia coli. Mol Gen Genet. 1976 Jun 15;145(3):273–280. doi: 10.1007/BF00325823. [DOI] [PubMed] [Google Scholar]
  23. Sugino A., Higgins N. P., Brown P. O., Peebles C. L., Cozzarelli N. R. Energy coupling in DNA gyrase and the mechanism of action of novobiocin. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4838–4842. doi: 10.1073/pnas.75.10.4838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sumida-Yasumoto C., Yudelevich A., Hurwitz J. DNA synthesis in vitro dependent upon phiX174 replicative form I DNA. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1887–1891. doi: 10.1073/pnas.73.6.1887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vosberg H. P., Hoffmann-Berling H. DNA synthesis in nucleotide-permeable Escherichia coli cells. I. Preparation and properties of ether-treated cells. J Mol Biol. 1971 Jun 28;58(3):739–753. doi: 10.1016/0022-2836(71)90037-4. [DOI] [PubMed] [Google Scholar]
  26. Wechsler J. A., Gross J. D. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol Gen Genet. 1971;113(3):273–284. doi: 10.1007/BF00339547. [DOI] [PubMed] [Google Scholar]
  27. Wickner R. B., Hurwitz J. DNA replication in Escherichia coli made permeable by treatment with high sucrose. Biochem Biophys Res Commun. 1972 Apr 14;47(1):202–211. doi: 10.1016/s0006-291x(72)80029-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES