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. 1981 Mar;145(3):1428–1431. doi: 10.1128/jb.145.3.1428-1431.1981

Arylsulfatase from Pseudomonas sp. strain C12B: purification to homogeneity, immunological analysis, and physical properties.

J R George, J W Fitzgerald
PMCID: PMC217151  PMID: 7204346

Abstract

Arylsulfatase was purified 219-fold from Pseudomonas sp. strain C12B. The final preparation was homogeneous by electrophoretic and immunological analysis. The enzyme is a monomer of molecular weight about 51,000, with a Stokes radius of 3.0 X 10(-7) cm, a frictional ratio of 1.2, and a sedimentation coefficient of 4.1S.

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Selected References

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  1. Adachi T., Murooka Y., Harada T. Regulation of arylsulfatase synthesis by sulfur compounds in Klebsiella aerogenes. J Bacteriol. 1975 Jan;121(1):29–35. doi: 10.1128/jb.121.1.29-35.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burns G. R., Galanopoulou E., Wynn C. H. Kinetic studies of the phenol sulphate-phenol sulphotransferase of Aspergillus oryzae. Biochem J. 1977 Oct 1;167(1):223–227. doi: 10.1042/bj1670223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burns G. R., Wynn C. H. Studies on the Arylsulphatase and phenol sulphotransferase activities of Aspergillus oryzae. Biochem J. 1975 Sep;149(3):697–705. doi: 10.1042/bj1490697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fitzgerald J. W., George J. R. Localization of arylsulfatase in Pseudomonas C12B. Appl Environ Microbiol. 1977 Jul;34(1):107–108. doi: 10.1128/aem.34.1.107-108.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fitzgerald J. W. Sulfate ester formation and hydrolysis: a potentially important yet often ignored aspect of the sulfur cycle of aerobic soils. Bacteriol Rev. 1976 Sep;40(3):698–721. doi: 10.1128/br.40.3.698-721.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Henderson M. J., Milazzo F. H. Arylsulfatase in Salmonella typhimurium: detection and influence of carbon source and tyramine on its synthesis. J Bacteriol. 1979 Jul;139(1):80–87. doi: 10.1128/jb.139.1.80-87.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  8. Murooka Y., Higashiura T., Harada T. Genetic mapping of tyramine oxidase and arylsulfatase genes and their regulation in intergeneric hybrids of enteric bacteria. J Bacteriol. 1978 Nov;136(2):714–722. doi: 10.1128/jb.136.2.714-722.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murooka Y., Yamada T., Tanabe S., Harada T. Immunological study of the regulation of cellular arylsulfatase synthesis in Klebsiella aerogenes. J Bacteriol. 1977 Oct;132(1):247–253. doi: 10.1128/jb.132.1.247-253.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Murooka Y., Yim M. H., Harada T. Formation and Purification of Serratia marcescens Arylsulfatase. Appl Environ Microbiol. 1980 Apr;39(4):812–817. doi: 10.1128/aem.39.4.812-817.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oxenburgh M. S., Snoswell A. M. Use of streptomycin in the separation of nucleic acids from protein in a bacterial extract. Nature. 1965 Sep 25;207(5004):1416–1417. doi: 10.1038/2071416a0. [DOI] [PubMed] [Google Scholar]
  12. Payne W. J., Fitzgerald J. W., Dodgson K. S. Methods for visualization of enzymes in polyacrylamide gels. Appl Microbiol. 1974 Jan;27(1):154–158. doi: 10.1128/am.27.1.154-158.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RAMMLER D. H., GRADO C., FOWLER L. R. SULFUR METABOLISM OF AEROBACTER AEROGENES. I. A REPRESSIBLE SULFATASE. Biochemistry. 1964 Feb;3:224–230. doi: 10.1021/bi00890a014. [DOI] [PubMed] [Google Scholar]
  14. SIEGEL L. M., MONTY K. J. DETERMINATION OF MOLECULAR WEIGHTS AND FRICTIONAL RATIOS OF MACROMOLECULES IN IMPURE SYSTEMS: AGGREGATION OF UREASE. Biochem Biophys Res Commun. 1965 May 3;19:494–499. doi: 10.1016/0006-291x(65)90152-x. [DOI] [PubMed] [Google Scholar]
  15. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  16. Siegel L. M., Monty K. J. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases. Biochim Biophys Acta. 1966 Feb 7;112(2):346–362. doi: 10.1016/0926-6585(66)90333-5. [DOI] [PubMed] [Google Scholar]

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