Abstract
The folded chromosome or nucleoid of Escherichia coli was analyzed by low-speed sedimentation in neutral sucrose gradients after heat treatment (30 min at 50 degrees C) and subsequent incubation of cells at 37 degrees C for various times. Heat treatment resulted in in vivo association of the nucleoids with cellular protein and in an increase in sedimentation coefficient. During incubation at 37 degrees C, a fraction of the nucleoids, from heated cells, because dissociated from cellular protein and regained their characteristic sedimentation coefficients. The percentage of nucleoids which returned to their control sedimentation position in the sucrose gradients corresponded to the percentage of cells able to repair thermal damage as assayed by enumeration on agar plates.
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Selected References
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- Pellon J. R., Ulmer K. M., Gomez R. F. Heat damage to the chromosome of Escherichia coli K-12. Appl Environ Microbiol. 1980 Aug;40(2):358–364. doi: 10.1128/aem.40.2.358-364.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roti Roti J. L., Winward R. T. The effects of hyperthermia on the protein-to-DNA ratio of isolated HeLa cell chromatin. Radiat Res. 1978 Apr;74(1):159–169. [PubMed] [Google Scholar]