Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Mar;145(3):1459–1462. doi: 10.1128/jb.145.3.1459-1462.1981

Properties of the lactose transport system in Klebsiella sp. strain CT-1.

K Imai, B G Hall
PMCID: PMC217159  PMID: 6907272

Abstract

Highly purified [D-glucose-1-14C]lactose has been used to study the transport of lactose by Klebsiella sp. strain CT-1. Strain CT-1 transports lactose by a lactose-inducible system that exhibited an apparent Km of 6 mM lactose and an apparent Vmax of 140 nmol/min per mg of cell protein. Lactose uptake was inhibited competitively by o-nitrophenyl-beta-D-galactoside with a Ki value of 8 mM, but was not inhibited by thio-beta-methyl-galactoside. D-Glucose, D-mannose, 2-deoxyglucose, and alpha-methyl-D-glucoside also inhibited lactose uptake. Phosphoenolpyruvate-dependent hydrolysis of o-nitrophenyl-beta-D-galactoside and lactose-dependent release of pyruvate from phosphoenolpyruvate by benzene-treated CT-1 cells showed that CT-1 transports lactose by a phosphoenolpyruvate:sugar phosphotransferase system. Correlations between the growth rate of CT-1 on lactose and properties of the transport system indicated that transport is the rate limiting step in utilization of lactose.

Full text

PDF
1459

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adhya S., Echols H. Glucose effect and the galactose enzymes of Escherichia coli: correlation between glucose inhibition of induction and inducer transport. J Bacteriol. 1966 Sep;92(3):601–608. doi: 10.1128/jb.92.3.601-608.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bissett D. L., Anderson R. L. Genetic evidence for the physiological significance of the D-tagatose 6-phosphate pathway of lactose and D-galactose degradation in staphylococcus aureus. J Bacteriol. 1974 Sep;119(3):698–704. doi: 10.1128/jb.119.3.698-704.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark B., Holms W. H. Control of the sequential utilization of glucose and fructose by Escherichia coli. J Gen Microbiol. 1976 Aug;96(2):191–201. doi: 10.1099/00221287-95-2-191. [DOI] [PubMed] [Google Scholar]
  4. Hall B. G. Lactose metabolism involving phospho-beta-galactosidase in Klebsiella. J Bacteriol. 1979 Jun;138(3):691–698. doi: 10.1128/jb.138.3.691-698.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hall B. G. Properties of beta-galactosidase III: implications for entry of galactosides into Klebsiella. J Bacteriol. 1980 May;142(2):433–438. doi: 10.1128/jb.142.2.433-438.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hall B. G., Reeve E. C. A third beta-galactosidase in a strain of Klebsiella that possesses two lac genes. J Bacteriol. 1977 Oct;132(1):219–223. doi: 10.1128/jb.132.1.219-223.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hamilton I. R., Lo G. C. Co-induction of beta-galactosidase and the lactose-P-enolpyruvate phosphotransferase system in Streptococcus salivarius and Streptococcus mutans. J Bacteriol. 1978 Dec;136(3):900–908. doi: 10.1128/jb.136.3.900-908.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hartl D. L., Hall B. G. Second naturally occurring beta-galactosidase in E. coli. Nature. 1974 Mar 8;248(5444):152–153. doi: 10.1038/248152a0. [DOI] [PubMed] [Google Scholar]
  9. Koch A. L. Local and non-local interactions of fluxes mediated by the glucose and galactoside permeases of Escherichia coli. Biochim Biophys Acta. 1971 Oct 12;249(1):197–215. doi: 10.1016/0005-2736(71)90097-6. [DOI] [PubMed] [Google Scholar]
  10. Markwell J., Shimamoto G. T., Bissett D. L., Anderson R. L. Pathway of galactitol catabolism in Klebsiella pneumoniae. Biochem Biophys Res Commun. 1976 Jul 12;71(1):221–227. doi: 10.1016/0006-291x(76)90271-0. [DOI] [PubMed] [Google Scholar]
  11. Messer A. Lactose permeation via the arabinose transport system in Escherichia coli K-12. J Bacteriol. 1974 Oct;120(1):266–272. doi: 10.1128/jb.120.1.266-272.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Saier M. H., Jr, Roseman S. Sugar transport. 2nducer exclusion and regulation of the melibiose, maltose, glycerol, and lactose transport systems by the phosphoenolpyruvate:sugar phosphotransferase system. J Biol Chem. 1976 Nov 10;251(21):6606–6615. [PubMed] [Google Scholar]
  13. Simoni R. D., Roseman S. Sugar transport. VII. Lactose transport in Staphylococcus aureus. J Biol Chem. 1973 Feb 10;248(3):966–974. [PubMed] [Google Scholar]
  14. Wilson D. B. The regulation and properties of the galactose transport system in Escherichia coli K12. J Biol Chem. 1974 Jan 25;249(2):553–558. [PubMed] [Google Scholar]
  15. Winkler H. H., Wilson T. H. Inhibition of beta-galactoside transport by substrates of the glucose transport system in Escherichia coli. Biochim Biophys Acta. 1967;135(5):1030–1051. doi: 10.1016/0005-2736(67)90073-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES