Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Feb;145(2):681–686. doi: 10.1128/jb.145.2.681-686.1981

Properties of six pesticide degradation plasmids isolated from Alcaligenes paradoxus and Alcaligenes eutrophus.

R H Don, J M Pemberton
PMCID: PMC217166  PMID: 6257648

Abstract

Biophysical and genetic properties of six independently isolated plasmids encoding the degradation of the herbicides 2,4-dichlorophenoxyacetic acid and 4-chloro-2-methylphenoxyacetic acid are described. Four of the plasmids, pJP3, pJP4, pJP5, and pJP7, had molecular masses of 51 megadaltons, belonged to the IncP1 incompatibility group, and transferred freely to strains of Escherichia coli, Rhodopseudomonas sphaeroides, Rhizobium sp., Agrobacterium tumefaciens, Pseudomonas putida, Pseudomonas fluorescens, and Acinetobacter calcoaceticus. In addition, these four plasmids conferred resistance to merbromin, phenylmercury acetate, and mercuric ions, had almost identical restriction endonuclease cleavage patterns, and encoded degradation of m-chlorobenzoate. The two other plasmids, pJP2 and pJP9, did not belong to the IncP1 incompatibility group, had molecular masses of 37 megadaltons, encoded the degradation of phenoxyacetic acid, and possessed identical restriction endonuclease cleavage patterns.

Full text

PDF
681

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander M. Biodegradation: problems of molecular recalcitrance and microbial fallibility. Adv Appl Microbiol. 1965;7:35–80. doi: 10.1016/s0065-2164(08)70383-6. [DOI] [PubMed] [Google Scholar]
  2. Bayley S. A., Morris D. W., Broda P. The relationship of degradative and resistance plasmids of Pseudomonas belonging to the same incompatibility group. Nature. 1979 Jul 26;280(5720):338–339. doi: 10.1038/280338a0. [DOI] [PubMed] [Google Scholar]
  3. Chakrabarty A. M. Plasmids in Pseudomonas. Annu Rev Genet. 1976;10:7–30. doi: 10.1146/annurev.ge.10.120176.000255. [DOI] [PubMed] [Google Scholar]
  4. Cohen S. N. Transposable genetic elements and plasmid evolution. Nature. 1976 Oct 28;263(5580):731–738. doi: 10.1038/263731a0. [DOI] [PubMed] [Google Scholar]
  5. Danna K. J., Sack G. H., Jr, Nathans D. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome. J Mol Biol. 1973 Aug 5;78(2):363–376. doi: 10.1016/0022-2836(73)90122-8. [DOI] [PubMed] [Google Scholar]
  6. Duggleby C. J., Bayley S. A., Worsey M. J., Williams P. A., Broda P. Molecular sizes and relationships of TOL plasmids in Pseudomonas. J Bacteriol. 1977 Jun;130(3):1274–1280. doi: 10.1128/jb.130.3.1274-1280.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fisher P. R., Appleton J., Pemberton J. M. Isolation and characterization of the pesticide-degrading plasmid pJP1 from Alcaligenes paradoxus. J Bacteriol. 1978 Sep;135(3):798–804. doi: 10.1128/jb.135.3.798-804.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gilardi G. L. Characterization of nonfermentative nonfastidious gram negative bacteria encountered in medical bacteriology. J Appl Bacteriol. 1971 Sep;34(3):623–644. doi: 10.1111/j.1365-2672.1971.tb02326.x. [DOI] [PubMed] [Google Scholar]
  9. Grinsted J., Bennett P. M., Richmond M. H. A restriction enzyme map of R-plasmid RP1. Plasmid. 1977 Nov;1(1):34–37. doi: 10.1016/0147-619x(77)90006-3. [DOI] [PubMed] [Google Scholar]
  10. Haas D., Holloway B. W. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Mol Gen Genet. 1976 Mar 30;144(3):243–251. doi: 10.1007/BF00341722. [DOI] [PubMed] [Google Scholar]
  11. Hansen J. B., Olsen R. H. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5. J Bacteriol. 1978 Jul;135(1):227–238. doi: 10.1128/jb.135.1.227-238.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Heffron F., Sublett R., Hedges R. W., Jacob A., Falkow S. Origin of the TEM-beta-lactamase gene found on plasmids. J Bacteriol. 1975 Apr;122(1):250–256. doi: 10.1128/jb.122.1.250-256.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
  14. Jacoby G. A., Rogers J. E., Jacob A. E., Hedges R. W. Transposition of Pseudomonas toluene-degrading genes and expression in Escherichia coli. Nature. 1978 Jul 13;274(5667):179–180. doi: 10.1038/274179a0. [DOI] [PubMed] [Google Scholar]
  15. Pemberton J. M., Fisher P. R. 2,4-D plasmids and persistence. Nature. 1977 Aug 25;268(5622):732–733. doi: 10.1038/268732a0. [DOI] [PubMed] [Google Scholar]
  16. Reineke W., Knackmuss H. J. Construction of haloaromatics utilising bacteria. Nature. 1979 Feb 1;277(5695):385–386. doi: 10.1038/277385a0. [DOI] [PubMed] [Google Scholar]
  17. Stanisich V. A. The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. J Gen Microbiol. 1974 Oct;84(2):332–342. doi: 10.1099/00221287-84-2-332. [DOI] [PubMed] [Google Scholar]
  18. Weiss A. A., Murphy S. D., Silver S. Mercury and organomercurial resistances determined by plasmids in Staphylococcus aureus. J Bacteriol. 1977 Oct;132(1):197–208. doi: 10.1128/jb.132.1.197-208.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Williams P. A., Murray K. Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. J Bacteriol. 1974 Oct;120(1):416–423. doi: 10.1128/jb.120.1.416-423.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wong C. L., Dunn N. W. Transmissible plasmid coding for the degradation of benzoate and m-toluate in Pseudomonas arvilla mt-2. Genet Res. 1974 Apr;23(2):227–232. doi: 10.1017/s0016672300014853. [DOI] [PubMed] [Google Scholar]
  21. Yagi Y., Clewell D. B. Plasmid-determined tetracycline resistance in Streptococcus faecalis: tandemly repeated resistance determinants in amplified forms of pAMalpha1 DNA. J Mol Biol. 1976 Apr 15;102(3):583–600. doi: 10.1016/0022-2836(76)90336-3. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES