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. 1981 Feb;145(2):713–721. doi: 10.1128/jb.145.2.713-721.1981

Formation of Escherichia coli Hfr strains by integrative suppression with the P group plasmid RP1.

R R Martin, C L Thorlton, L Unger
PMCID: PMC217170  PMID: 7007346

Abstract

Hfr strains of Escherichia coli were obtained by integrative suppression of a dnaA(Ts) mutation by the Inc P-1 plasmid RP1 without prior creation of an unnatural homology between the plasmid and the E. coli chromosome. Unmodified RP1 mobilized the polarized transfer of the chromosome in a counterclock-wise direction from a distinct origin between 81 min (pyrE) and 82 min (dnaA) with pyrE as a leading marker. Inheritance of RP1-Hfr chromosomal and antibiotic resistance genes was due to recombination with the recipient chromosome, as shown by the need for a functional recA system. The acquisition of temperature resistance and donor ability was accompanied by the disappearance of free plasmid when the selection pressure for integration was maintained (growth at 41 degrees C); the loss of temperature resistance and donor ability was accompanied by the reappearance of autonomous RP1 when the selection pressure was removed (growth at 30 degrees C).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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