Abstract
Nitrogenase activities and the patterns of in vivo inhibition of nitrogenase by NH+4 were compared in Rhodospirillum rubrum grown under several conditions of nitrogen availability. In cells grown on N2 or glutamate plus N2, nitrogenase activity was relatively low and was totally inhibited by added NH+4 in 15 to 20 min. In contrast, cells grown on glutamate alone displayed higher nitrogenase activity, and NH+4 had very little effect. Cells grown on limiting amounts of NH+4 had lower nitrogenase activity, but NH+4 produced little inhibitory effect. Uptake of NH+4 could be demonstrated under all of these conditions, and this uptake was blocked by DL-methionine-dl-sulfoximine. The data indicated that cells not recently exposed to NH+4 had no mechanism for rapidly turning off nitrogenase activity in response to sudden additions of NH+4. In contrast, cells grown in the presence of N2, which form NH+4 internally, inhibited nitrogenase activity relatively quickly in response to added NH+4.
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- Carithers R. P., Yoch D. C., Arnon D. I. Two forms of nitrogenase from the photosynthetic bacterium Rhodospirillum rubrum. J Bacteriol. 1979 Feb;137(2):779–789. doi: 10.1128/jb.137.2.779-789.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chaykin S. Assay of nicotinamide deamidase. Determination of ammonia by the indophenol reaction. Anal Biochem. 1969 Oct 1;31(1):375–382. doi: 10.1016/0003-2697(69)90278-4. [DOI] [PubMed] [Google Scholar]
- Jones B. L., Monty K. J. Glutamine as a feedback inhibitor of the Rhodopseudomonas sphaeroides nitrogenase system. J Bacteriol. 1979 Sep;139(3):1007–1013. doi: 10.1128/jb.139.3.1007-1013.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ludden P. W., Burris R. H. Activating factor for the iron protein of nitrogenase from Rhodospirillum rubrum. Science. 1976 Oct 22;194(4263):424–426. doi: 10.1126/science.824729. [DOI] [PubMed] [Google Scholar]
- Ludden P. W., Burris R. H. Removal of an adenine-like molecule during activation of dinitrogenase reductase from Rhodospirillum rubrum. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6201–6205. doi: 10.1073/pnas.76.12.6201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nordlund S., Eriksson U. Nitrogenase from Rhodospirillum rubrum. Relation between 'switch-off' effect and the membrane component. Hydrogen production and acetylene reduction with different nitrogenase component ratios. Biochim Biophys Acta. 1979 Sep 11;547(3):429–437. doi: 10.1016/0005-2728(79)90023-9. [DOI] [PubMed] [Google Scholar]
- ORMEROD J. G., ORMEROD K. S., GEST H. Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys. 1961 Sep;94:449–463. doi: 10.1016/0003-9861(61)90073-x. [DOI] [PubMed] [Google Scholar]
- Ronzio R. A., Rowe W. B., Meister A. Studies on the mechanism of inhibition of glutamine synthetase by methionine sulfoximine. Biochemistry. 1969 Mar;8(3):1066–1075. doi: 10.1021/bi00831a038. [DOI] [PubMed] [Google Scholar]
- Schick H. J. Substrate and light dependent fixation of molecular nitrogen in Rhodospirillum rubrum. Arch Mikrobiol. 1971;75(2):89–101. doi: 10.1007/BF00407997. [DOI] [PubMed] [Google Scholar]
- Sweet W. J., Houchins J. P., Rosen P. R., Arp D. J. Polarographic measurement of H2 in aqueous solutions. Anal Biochem. 1980 Sep 15;107(2):337–340. doi: 10.1016/0003-2697(80)90393-0. [DOI] [PubMed] [Google Scholar]
- Yoch D. C., Cantu M. Changes in the regulatory form of Rhodospirillum rubrum nitrogenase as influenced by nutritional and environmental factors. J Bacteriol. 1980 Jun;142(3):899–907. doi: 10.1128/jb.142.3.899-907.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoch D. C. Regulation of nitrogenase A and R concentrations in Rhodopseudomonas capsulata by glutamine synthetase. Biochem J. 1980 Apr 1;187(1):273–276. doi: 10.1042/bj1870273. [DOI] [PMC free article] [PubMed] [Google Scholar]