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. 1981 Feb;145(2):904–913. doi: 10.1128/jb.145.2.904-913.1981

Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12.

N Schaus, K O'Day, W Peters, A Wright
PMCID: PMC217197  PMID: 7007354

Abstract

Amber mutants with defects in the dnaA gene of Escherichia coli K-12 were isolated after localized mutagenesis of the tna-dnaA region of the chromosome. We isolated 36 mutants defective in the initiation of deoxyribonucleic acid replication as determined by their dependence upon integrative suppression by a P2 sig5 prophage. Three of the 36 mutants were shown to contain amber mutations through the use of a temperature-sensitive amber suppressor. These mutations, which mapped between gyrB and tna, were characterized genetically and biochemically as amber mutations in dnaA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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