Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Feb;145(2):974–979. doi: 10.1128/jb.145.2.974-979.1981

Replication of M13 oriC bacteriophages in Escherichia coli rep mutant is dependent on the cloned Escherichia coli replication origin.

L S Kaguni, J M Kaguni, D S Ray
PMCID: PMC217206  PMID: 6257657

Abstract

The involvement of the Escherichia coli rep protein in the replication of M13 chimeric deoxyribonucleic acids (DNAs) carrying the E. coli chromosomal DNA replication origin (oriC) has been examined. Previous studies indicate that the cloning of a 3,550-base-pair sequence of chromosomal DNA containing oriC into an M13 vector allows extensive replication of the M13 oriC chimeric DNA in an E. coli rep-3 mutant. We have extended these studies by preparing a 330-base-pair deletion that specifically deletes the oriC sequence in the M13 oriC DNAs, to demonstrate that the replication observed in the rep-3 host is dependent on the cloned origin. Thus, a DNA-unwinding enzyme other than the rep protein may be involved in the strand separation process accompanying replication which initiates at oriC in the M13 oriC chimeric DNAs and in the E. coli chromosome. The rep assay used for assessing the functionality of the cloned oriC is useful for analysis of any rep-independent origin of replication functional in E. coli. A direct selection for a cloned origin of replication is possible in the rep-3 recA56 host. Since the cloned origin is nonessential for propagation of the M13 chimeric phage in a rep+ host, mutations in the cloned origin may be constructed, and the mutant phage may be examined by a simple transductional analysis of the rep-3 recA56 mutant strain.

Full text

PDF
974

Images in this article

977Image
on p.977

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdel-Monem M., Dürwald H., Hoffmann-Berling H. DNA unwinding enzyme II of Escherichia coli. 2. Characterization of the DNA unwinding activity. Eur J Biochem. 1977 Sep 15;79(1):39–45. doi: 10.1111/j.1432-1033.1977.tb11781.x. [DOI] [PubMed] [Google Scholar]
  2. Abdel-Monem M., Dürwald H., Hoffmann-Berling H. Enzymic unwinding of DNA. 2. Chain separation by an ATP-dependent DNA unwinding enzyme. Eur J Biochem. 1976 Jun 1;65(2):441–449. doi: 10.1111/j.1432-1033.1976.tb10359.x. [DOI] [PubMed] [Google Scholar]
  3. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  5. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Denhardt D. T., Dressler D. H., Hathaway A. THE ABORTIVE REPLICATION OF PhiX174 DNA IN A RECOMBINATION-DEFICIENT MUTANT OF Escherichia coli. Proc Natl Acad Sci U S A. 1967 Mar;57(3):813–820. doi: 10.1073/pnas.57.3.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Godson G. N., Barrell B. G., Staden R., Fiddes J. C. Nucleotide sequence of bacteriophage G4 DNA. Nature. 1978 Nov 16;276(5685):236–247. doi: 10.1038/276236a0. [DOI] [PubMed] [Google Scholar]
  8. Herrmann R., Neugebauer K., Pirkl E., Zentgraf H., Schaller H. Conversion of bacteriophage fd into an efficient single-stranded DNA vector system. Mol Gen Genet. 1980 Jan;177(2):231–242. doi: 10.1007/BF00267434. [DOI] [PubMed] [Google Scholar]
  9. Herrmann R., Neugebauer K., Zentgraf H., Schaller H. Transposition of a DNA sequence determining kanamycin resistance into the single-stranded genome of bacteriophage fd. Mol Gen Genet. 1978 Feb 16;159(2):171–178. doi: 10.1007/BF00270890. [DOI] [PubMed] [Google Scholar]
  10. Hines J. C., Ray D. S. Construction and characterization of new coliphage M13 cloning vectors. Gene. 1980 Nov;11(3-4):207–218. doi: 10.1016/0378-1119(80)90061-x. [DOI] [PubMed] [Google Scholar]
  11. Hirota Y., Yasuda S., Yamada M., Nishimura A., Sugimoto K., Sugisaki H., Oka A., Takanami M. Structural and functional properties of the Escherichia coli origin of DNA replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):129–138. doi: 10.1101/sqb.1979.043.01.019. [DOI] [PubMed] [Google Scholar]
  12. Kaguni J., LaVerne L. S., Ray D. S. Cloning and expression of the Escherichia coli replication origin in a single-stranded DNA phage. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6250–6254. doi: 10.1073/pnas.76.12.6250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kaguni J., Ray D. S. Cloning of a functional replication origin of phage G4 into the genome of phage M13. J Mol Biol. 1979 Dec 25;135(4):863–878. doi: 10.1016/0022-2836(79)90516-3. [DOI] [PubMed] [Google Scholar]
  14. Lane H. E., Denhardt D. T. The rep mutation. IV. Slower movement of replication forks in Escherichia coli rep strains. J Mol Biol. 1975 Sep 5;97(1):99–112. doi: 10.1016/s0022-2836(75)80025-8. [DOI] [PubMed] [Google Scholar]
  15. Messer W., Bergmans H. E., Meijer M., Womack J. E., Hansen F. G., von Meyenburg K. Mini-chromosomes: plasmids which carry the E. coli replication origin. Mol Gen Genet. 1978 Jul 4;162(3):269–275. doi: 10.1007/BF00268852. [DOI] [PubMed] [Google Scholar]
  16. Messer W., Meijer M., Bergmans H. E., Hansen F. G., von Meyenburg K., Beck E., Schaller H. Origin of replication, oriC, of the Escherichia coli K12 chromosome: nucleotide sequence. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):139–145. doi: 10.1101/sqb.1979.043.01.020. [DOI] [PubMed] [Google Scholar]
  17. Miki T., Hiraga S., Nagata T., Yura T. Bacteriophage lambda carrying the Escherichia coli chromosomal region of the replication origin. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5099–5103. doi: 10.1073/pnas.75.10.5099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Oka A., Sugimoto K., Takanami M., Hirota Y. Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication. Mol Gen Genet. 1980 Apr;178(1):9–20. doi: 10.1007/BF00267207. [DOI] [PubMed] [Google Scholar]
  19. Ray D. S., Kook K. Insertion of the Tn3 transposon into the genome of the single-stranded DNA phage M13. Gene. 1978 Oct;4(2):109–119. doi: 10.1016/0378-1119(78)90024-0. [DOI] [PubMed] [Google Scholar]
  20. Richet E., Kohiyama M. Purification and characterization of a DNA-dependent ATPase from Escherichia coli. J Biol Chem. 1976 Feb 10;251(3):808–812. [PubMed] [Google Scholar]
  21. Scott J. F., Eisenberg S., Bertsch L. L., Kornberg A. A mechanism of duplex DNA replication revealed by enzymatic studies of phage phi X174: catalytic strand separation in advance of replication. Proc Natl Acad Sci U S A. 1977 Jan;74(1):193–197. doi: 10.1073/pnas.74.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shortle D., Nathans D. Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome. Proc Natl Acad Sci U S A. 1978 May;75(5):2170–2174. doi: 10.1073/pnas.75.5.2170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sugden B., De Troy B., Roberts R. J., Sambrook J. Agarose slab-gel electrophoresis equipment. Anal Biochem. 1975 Sep;68(1):36–46. doi: 10.1016/0003-2697(75)90676-4. [DOI] [PubMed] [Google Scholar]
  24. Sugimoto K., Oka A., Sugisaki H., Takanami M., Nishimura A., Yasuda Y., Hirota Y. Nucleotide sequence of Escherichia coli K-12 replication origin. Proc Natl Acad Sci U S A. 1979 Feb;76(2):575–579. doi: 10.1073/pnas.76.2.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tessman E. S. Mutants of bacteriophage S13 blocked in infectious DNA synthesis. J Mol Biol. 1966 May;17(1):218–236. doi: 10.1016/s0022-2836(66)80104-3. [DOI] [PubMed] [Google Scholar]
  26. Yarranton G. T., Das R. H., Gefter M. L. Enzyme-catalyzed DNA unwinding. A DNA-dependent ATPase from E. coli. J Biol Chem. 1979 Dec 10;254(23):11997–12001. [PubMed] [Google Scholar]
  27. Yarranton G. T., Das R. H., Gefter M. L. Enzyme-catalyzed DNA unwinding. Mechanism of action of helicase III. J Biol Chem. 1979 Dec 10;254(23):12002–12006. [PubMed] [Google Scholar]
  28. Yarranton G. T., Gefter M. L. Enzyme-catalyzed DNA unwinding: studies on Escherichia coli rep protein. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1658–1662. doi: 10.1073/pnas.76.4.1658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yasuda S., Hirota Y. Cloning and mapping of the replication origin of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5458–5462. doi: 10.1073/pnas.74.12.5458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. de Vries F. A., Collins C. J., Jackson D. A. Joining of simian virus 40 DNA molecules at endonuclease R Eco Ri sites by polynucleotide ligase and analysis of the products by agarose gel electrophoresis. Biochim Biophys Acta. 1976 Jul 2;435(3):213–227. doi: 10.1016/0005-2787(76)90103-9. [DOI] [PubMed] [Google Scholar]
  31. van Wezenbeek P. M., Hulsebos T. J., Schoenmakers J. G. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene. 1980 Oct;11(1-2):129–148. doi: 10.1016/0378-1119(80)90093-1. [DOI] [PubMed] [Google Scholar]
  32. von Meyenburg K., Hansen F. G., Nielsen L. D., Jørgensen P. Origin of replication, oriC, of the Escherichia coli chromosome: mapping of genes relative to R.EcoRI cleavage sites in the oriC region. Mol Gen Genet. 1977 Dec 14;158(1):101–109. doi: 10.1007/BF00455124. [DOI] [PubMed] [Google Scholar]
  33. von Meyenburg K., Hansen F. G., Nielsin L. D., Riise E. Origin of replication, oriC, or the Escherichia coli chromosome on specialized transducing phages lambda asn. Mol Gen Genet. 1978 Apr 17;160(3):287–295. doi: 10.1007/BF00332972. [DOI] [PubMed] [Google Scholar]
  34. von Meyenburg K., Hansen F. G., Riise E., Bergmans H. E., Meijer M., Messer W. Origin of replication, oriC, of the Escherichia coli K12 chromosome: genetic mapping and minichromosome replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):121–128. doi: 10.1101/sqb.1979.043.01.018. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES