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. 1981 Feb;145(2):1018–1024. doi: 10.1128/jb.145.2.1018-1024.1981

Identification and preliminary characterization of Vibrio cholerae outer membrane proteins.

J T Kelley, C D Parker
PMCID: PMC217212  PMID: 7462157

Abstract

Outer membrane proteins of Vibrio cholerae were purified by sucrose density centrifugation and Triton X-100 extraction at 10 mM Mg2+. The proteins were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. V. cholerae outer membrane proteins presented a unique pattern when compared with the patterns of other gram-negative rods. There were 8 to 10 major bands (Mr 94,000 to 27,000), with most of the protein located in band 5 (Mr approximately 45,000), which thus appears to be the major structural protein of the outer membrane. Lipid and carbohydrate were associated with band 6.

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Selected References

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