Abstract
The growth of Escherichia coli cells has been analyzed for the first time by dual-parameter flow cytophotometry, in which the deoxyribonucleic acid and protein contents of single bacteria have been measured simultaneously with an accuracy of a few percent and at a rate of 3,000 cells/s.
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Selected References
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- Bailey J. E., Fazel-Makjlessi J., McQuitty D. N., Lee Y. N., Allred J. C., Oro J. A. Characterization of bacterial growth by means of flow microfluorometry. Science. 1977 Dec 16;198(4322):1175–1176. doi: 10.1126/science.412254. [DOI] [PubMed] [Google Scholar]
- Barlogie B., Spitzer G., Hart J. S., Johnston D. A., Büchner T., Schumann J., Drewinko B. DNA histogram analysis of human hemopoietic cells. Blood. 1976 Aug;48(2):245–258. [PubMed] [Google Scholar]
- Boye E., Krisch R. E. Induction and repair of double- and single-strand DNA breaks in bacteriophage lambda superinfecting Escherichia coli. Int J Radiat Biol Relat Stud Phys Chem Med. 1980 Feb;37(2):119–133. doi: 10.1080/09553008014550191. [DOI] [PubMed] [Google Scholar]
- Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
- Jones N. C., Donachie W. D. Chromosome replication, transcription and control of cell division in Escherichia coli. Nat New Biol. 1973 May 23;243(125):100–103. [PubMed] [Google Scholar]
- Kubitschek H. E., Freedman M. L. Chromosome replication and the division cycle of Escherichia coli B-r. J Bacteriol. 1971 Jul;107(1):95–99. doi: 10.1128/jb.107.1.95-99.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lark K. G. Initiation and control of DNA synthesis. Annu Rev Biochem. 1969;38:569–604. doi: 10.1146/annurev.bi.38.070169.003033. [DOI] [PubMed] [Google Scholar]
- Lark K. G., Renger H. Initiation of DNA replication in Escherichia coli 15T-: chronological dissection of three physiological processes required for initiation. J Mol Biol. 1969 Jun 14;42(2):221–235. doi: 10.1016/0022-2836(69)90039-4. [DOI] [PubMed] [Google Scholar]
- Messer W. Initiation of deoxyribonucleic acid replication in Escherichia coli B-r: chronology of events and transcriptional control of initiation. J Bacteriol. 1972 Oct;112(1):7–12. doi: 10.1128/jb.112.1.7-12.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paau A. S., Cowles J. R., Oro J. Flow-microfluorometric analysis of Escherichia coli, Rhizobium meliloti, and Rhizobium japonicum at different stages of the growth cycle. Can J Microbiol. 1977 Sep;23(9):1165–1169. doi: 10.1139/m77-175. [DOI] [PubMed] [Google Scholar]
- Paau A. S., Lee D., Cowles J. R. Comparison of nucleic acid content in populations of free-living and symbiotic Rhizobium meliloti by flow microfluorometry. J Bacteriol. 1977 Feb;129(2):1156–1158. doi: 10.1128/jb.129.2.1156-1158.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pato M. L. Alterations of the rate of movement of deoxyribonucleic acid replication forks. J Bacteriol. 1975 Jul;123(1):272–277. doi: 10.1128/jb.123.1.272-277.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pierucci O., Helmstetter C. E. Chromosome replication, protein synthesis and cell division in Escherichia coli. Fed Proc. 1969 Nov-Dec;28(6):1755–1760. [PubMed] [Google Scholar]
- Steen H. B., Boye E. Bacterial growth studied by flow cytometry. Cytometry. 1980 Jul;1(1):32–36. doi: 10.1002/cyto.990010108. [DOI] [PubMed] [Google Scholar]
- Steen H. B. Further developments of a microscope-based flow cytometer: light scatter detection and excitation intensity compensation. Cytometry. 1980 Jul;1(1):26–31. doi: 10.1002/cyto.990010107. [DOI] [PubMed] [Google Scholar]
- Steen H. B., Lindmo T. Flow cytometry: a high-resolution instrument for everyone. Science. 1979 Apr 27;204(4391):403–404. doi: 10.1126/science.441727. [DOI] [PubMed] [Google Scholar]