Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Jan;145(1):138–144. doi: 10.1128/jb.145.1.138-144.1981

Structure of the heptose region of lipopolysaccharies from Rhodospirillum tenue.

J Radziejewska-Lebrecht, U Feige, H Mayer, J Weckesser
PMCID: PMC217254  PMID: 7462142

Abstract

There is a common structure (core region) in the lipopolysaccharides of Rhodospirillum tenue. It is composed of a branched trisaccharide of L-glycero-D-mannoheptose (and of 2-keto-3-deoxyoctonate), as revealed by methylation analyses of degraded polysaccharides of four different R. tenue strains. The structure is similar or might even be identical to the inner core of enterobacterial O antigens. In addition, each of the four R. tenue lipopolysaccharides contains a strain-specific region that consists of heptose(s) (L-glycero-D-mannoheptose or D-glycero-D-mannoheptose or both) or hexoses. There is a partial substitution of the core region and the strain-specific region by phosphorus, showing microheterogeneity.

Full text

PDF
138

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahamed N. M., Radziejewska-Lebrecht J., Widemann C., Mayer H. Reactivity of isolated lipopolysaccharides of enterobacterial R mutants with complete or incomplete core structures with lectins. Zentralbl Bakteriol A. 1980;247(4):468–482. [PubMed] [Google Scholar]
  2. Chester I. R., Meadow P. M. Heterogeneity of the lipopolysaccharide from Pseudomonas aeruginosa. Eur J Biochem. 1975 Oct 15;58(2):273–282. doi: 10.1111/j.1432-1033.1975.tb02373.x. [DOI] [PubMed] [Google Scholar]
  3. Feige U., Stirm S. On the structure of the Escherichia coli C cell wall lipopolysaccharide core and on its phiX174 receptor region. Biochem Biophys Res Commun. 1976 Jul 26;71(2):566–573. doi: 10.1016/0006-291x(76)90824-x. [DOI] [PubMed] [Google Scholar]
  4. Fromme I., Schlecht S. Einflubb der Belüftungsbedingungen auf die chemische Zdsammensetiung von Salmonella-Lipopolysacchariden. Zentralbl Bakteriol Orig A. 1973 Aug;224(3):331–344. [PubMed] [Google Scholar]
  5. Galanos C., Lüderitz O., Westphal O. A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969 Jun;9(2):245–249. doi: 10.1111/j.1432-1033.1969.tb00601.x. [DOI] [PubMed] [Google Scholar]
  6. Galanos C., Roppel J., Weckesser J., Rietschel E. T., Mayer H. Biological activities of lipopolysaccharides and lipid A from Rhodospirillaceae. Infect Immun. 1977 May;16(2):407–412. doi: 10.1128/iai.16.2.407-412.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
  8. Hurlbert R. E., Weckesser J., Tharanathan R. N., Mayer H. Isolation and characterization of the lipopolysaccharide of Thiocapsa roseopersicina. Eur J Biochem. 1978 Oct;90(2):241–246. doi: 10.1111/j.1432-1033.1978.tb12596.x. [DOI] [PubMed] [Google Scholar]
  9. Kotełko K., Gromska W., Papierz M., Sidorczyk Z., Krajewska D., Szer K. Core region in Proteus mirabilis lipopolysaccharide. J Hyg Epidemiol Microbiol Immunol. 1977;21(3):271–284. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROBERTS N. R., LEINER K. Y., WU M. L., FARR A. L. The quantitative histochemistry of brain. I. Chemical methods. J Biol Chem. 1954 Mar;207(1):1–17. [PubMed] [Google Scholar]
  11. Lüderitz O., Galanos C., Lehmann V., Mayer H., Rietschel E. T., Weckesser J. Chemical structure and biological activities of lipid A's from various bacterial families. Naturwissenschaften. 1978 Nov;65(11):578–585. doi: 10.1007/BF00364907. [DOI] [PubMed] [Google Scholar]
  12. Orskov I., Orskov F., Jann B., Jann K. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977 Sep;41(3):667–710. doi: 10.1128/br.41.3.667-710.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Prehm P., Stirm S., Jann B., Jann K. Cell-wall lipopolysaccharide from Escherichia coli B. Eur J Biochem. 1975 Aug 1;56(1):41–55. doi: 10.1111/j.1432-1033.1975.tb02205.x. [DOI] [PubMed] [Google Scholar]
  14. Radziejewska-Lebrecht J., Feige U., Jensen M., Kotełko K., Friebolin H., Mayer H. Structural studies on the glucose-heptose region of the Proteus mirabilis R core. Eur J Biochem. 1980;107(1):31–38. doi: 10.1111/j.1432-1033.1980.tb04620.x. [DOI] [PubMed] [Google Scholar]
  15. Schmidt G., Fromme I., Mayer H. Immunochemical studies on core lipopolysaccharides of Enterobacteriaceae of different genera. Eur J Biochem. 1970 Jun;14(2):357–366. doi: 10.1111/j.1432-1033.1970.tb00297.x. [DOI] [PubMed] [Google Scholar]
  16. Tharanathan R. N., Mayer H. Location of O-methyl sugars in antigenic (lipo-)polysaccharides of photosynthetic bacteria and cyanobacteria. Biochem J. 1978 May 1;171(2):403–408. doi: 10.1042/bj1710403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tharanathan R. N., Weckesser J., Mayer H. Structural studies on the D-arabinose-containing lipid A from Rhodospirillum tenue 2761. Eur J Biochem. 1978 Mar 15;84(2):385–394. doi: 10.1111/j.1432-1033.1978.tb12179.x. [DOI] [PubMed] [Google Scholar]
  18. WARAVDEKAR V. S., SASLAW L. D. A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of the malonaldehyde-thiobarbituric acid reaction. J Biol Chem. 1959 Aug;234(8):1945–1950. [PubMed] [Google Scholar]
  19. Weckesser J., Drews G., Indira R., Mayer H. Lipophilic O-antigens in Rhodospirillum tenue. J Bacteriol. 1977 May;130(2):629–634. doi: 10.1128/jb.130.2.629-634.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weckesser J., Drews G., Mayer H. Lipopolysaccharides of photosynthetic prokaryotes. Annu Rev Microbiol. 1979;33:215–239. doi: 10.1146/annurev.mi.33.100179.001243. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES