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. 1981 Jan;145(1):348–357. doi: 10.1128/jb.145.1.348-357.1981

Regulation of nitrogen fixation in Klebsiella pneumoniae: isolation and characterization of strains with nif-lac fusions.

D MacNeil, J Zhu, W J Brill
PMCID: PMC217279  PMID: 7007318

Abstract

Strains with lac fused to each of the seven nif operons were isolated by two different methods. Repressing conditions prevented expression of all nif operons, whereas derepressing conditions led to the expression of all nif operons. No differences in Nif regulation were observed between Escherichia coli and Klebsiella pneumoniae with the same nif-lac fusions. Most derivatives of nif-lac fusion strains selected on lactose and NH4+ contained nif operator mutations. Some derivative contained deletions, which establishes that the direction of transcription of all seven nif operons is toward his

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Selected References

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