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. 1981 Jan;145(1):472–478. doi: 10.1128/jb.145.1.472-478.1981

Lipid synthesis during the Escherichia coli cell cycle.

C E Carty, L O Ingram
PMCID: PMC217296  PMID: 7007325

Abstract

Lipid synthesis was examined in Escherichia coli cells at different stage of cell division. Exponentially growing cells were pulse-labeled with appropriate isotopes for 0.1 generation time, inactivated, and separated by size on a sucrose gradient. An abrupt increase in the rate of lipid synthesis occurred which was coincident with the initiation of cross walls. In contrast, the rate of protein synthesis during this same interval remained constant, resulting in an increased lipid/protein ratio in dividing cells. No changes in the composition of phospholipid head groups, fatty acids, or phospholipid molecular species were observed in cells at different stages of division. The observed increase in the rate of lipid synthesis may reflect a means by which the activities of membrane-associated enzymes are modulated during cross wall formation.

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Selected References

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  1. Ballesta J. P., Schaechter M. Effect of shift-down and growth inhibition on phospholipid metabolism of Escherichia coli. J Bacteriol. 1971 Jul;107(1):251–258. doi: 10.1128/jb.107.1.251-258.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bauza M. T., De Loach J. R., Aguanno J. J., Larrabee A. R. Acyl carrier protein prosthetic group exchange and phospholipid synthesis in synchronized cultures of a pantothenate auxotroph Escherichia coli. Arch Biochem Biophys. 1976 May;174(1):344–349. doi: 10.1016/0003-9861(76)90354-4. [DOI] [PubMed] [Google Scholar]
  3. Beck B. D., Park J. T. Activity of three murein hydrolases during the cell division cycle of Escherichia coli K-12 as measured in toluene-treated cells. J Bacteriol. 1976 Jun;126(3):1250–1260. doi: 10.1128/jb.126.3.1250-1260.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berger B., Carty C. E., Ingram L. O. Alcohol-induced changes in the phospholipid molecular species of Escherichia coli. J Bacteriol. 1980 Jun;142(3):1040–1044. doi: 10.1128/jb.142.3.1040-1044.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burdett I. D., Murray R. G. Septum formation in Escherichia coli: characterization of septal structure and the effects of antibiotics on cell division. J Bacteriol. 1974 Jul;119(1):303–324. doi: 10.1128/jb.119.1.303-324.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Buttke T. M., Ingram L. O. Inhibition of unsaturated fatty acid synthesis in escherichia coli by the antibiotic cerulenin. Biochemistry. 1978 Nov 28;17(24):5282–5286. doi: 10.1021/bi00617a031. [DOI] [PubMed] [Google Scholar]
  7. Buttke T. M., Ingram L. O. Mechanism of ethanol-induced changes in lipid composition of Escherichia coli: inhibition of saturated fatty acid synthesis in vivo. Biochemistry. 1978 Feb 21;17(4):637–644. doi: 10.1021/bi00597a012. [DOI] [PubMed] [Google Scholar]
  8. Churchward G. G., Holland I. B. Envelope synthesis during the cell cycle in Escherichia coli B/r. J Mol Biol. 1976 Aug 5;105(2):245–261. doi: 10.1016/0022-2836(76)90110-8. [DOI] [PubMed] [Google Scholar]
  9. Clark D. J. The regulation of DNA replication and cell division in E. coli B-r. Cold Spring Harb Symp Quant Biol. 1968;33:823–838. doi: 10.1101/sqb.1968.033.01.094. [DOI] [PubMed] [Google Scholar]
  10. Coleman R. Membrane-bound enzymes and membrane ultrastructure. Biochim Biophys Acta. 1973 Apr 3;300(1):1–30. doi: 10.1016/0304-4157(73)90010-5. [DOI] [PubMed] [Google Scholar]
  11. Cronan J. E., Jr Molecular biology of bacterial membrane lipids. Annu Rev Biochem. 1978;47:163–189. doi: 10.1146/annurev.bi.47.070178.001115. [DOI] [PubMed] [Google Scholar]
  12. Daniels M. J. Lipid synthesis in relation to the cell cycle of Bacillus megaterium KM and Escherichia coli. Biochem J. 1969 Dec;115(4):697–701. doi: 10.1042/bj1150697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. DiRienzo J. M., Inouye M. Lipid fluidity-dependent biosynthesis and assembly of the outer membrane proteins of E. coli. Cell. 1979 May;17(1):155–161. doi: 10.1016/0092-8674(79)90303-9. [DOI] [PubMed] [Google Scholar]
  14. DiRienzo J. M., Nakamura K., Inouye M. The outer membrane proteins of Gram-negative bacteria: biosynthesis, assembly, and functions. Annu Rev Biochem. 1978;47:481–532. doi: 10.1146/annurev.bi.47.070178.002405. [DOI] [PubMed] [Google Scholar]
  15. Fraley R. T., Lueking D. R., Kaplan S. The relationship of intracytoplasmic membrane assembly to the cell division cycle in Rhodopseudomonas sphaeroides. J Biol Chem. 1979 Mar 25;254(6):1980–1986. [PubMed] [Google Scholar]
  16. Gennis R. B., Strominger J. L. Activation of C55-isoprenoid alcohol phosphokinase from Staphylococcus aureus. I. Activation by phospholipids and fatty acids. J Biol Chem. 1976 Mar 10;251(5):1264–1269. [PubMed] [Google Scholar]
  17. Grisham C. M., Barnett R. E. The role of lipid-phase transitions in the regulation of the (sodium + potassium) adenosine triphosphatase. Biochemistry. 1973 Jul 3;12(14):2635–2637. doi: 10.1021/bi00738a013. [DOI] [PubMed] [Google Scholar]
  18. Hakenbeck R., Messer W. Activity of murein hydrolases in synchronized cultures of Escherichia coli. J Bacteriol. 1977 Mar;129(3):1239–1244. doi: 10.1128/jb.129.3.1239-1244.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hakenbeck R., Messer W. Oscillations in the synthesis of cell wall components in synchronized cultures of Escherichia coli. J Bacteriol. 1977 Mar;129(3):1234–1238. doi: 10.1128/jb.129.3.1234-1238.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Helmstetter C. E., Pierucci O. DNA synthesis during the division cycle of three substrains of Escherichia coli B/r. J Mol Biol. 1976 Apr 15;102(3):477–486. doi: 10.1016/0022-2836(76)90329-6. [DOI] [PubMed] [Google Scholar]
  21. Hidalgo C., Ikemoto N., Gergely J. Role of phospholipids in the calcium-dependent ATPase of the sarcoplasmic reticulum. Enzymatic and ESR studies with phospholipid-replaced membranes. J Biol Chem. 1976 Jul 25;251(14):4224–4232. [PubMed] [Google Scholar]
  22. Hoffmann B., Messer W., Schwarz U. Regulation of polar cap formation in the life cycle of Escherichia coli. J Supramol Struct. 1972;1(1):29–37. doi: 10.1002/jss.400010105. [DOI] [PubMed] [Google Scholar]
  23. Ingram L. O. Adaptation of membrane lipids to alcohols. J Bacteriol. 1976 Feb;125(2):670–678. doi: 10.1128/jb.125.2.670-678.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ingram L. O. Preferential inhibition of phosphatidyl ethanolamine synthesis in E. coli by alcohols. Can J Microbiol. 1977 Jun;23(6):779–789. doi: 10.1139/m77-115. [DOI] [PubMed] [Google Scholar]
  25. Johnson J. H., Grula E. A. Cell membrane phospholipids and their constitutent fatty acids in dividing and nondividing cells of Micrococcus lysodeikticus. Can J Microbiol. 1980 Jun;26(6):658–663. doi: 10.1139/m80-115. [DOI] [PubMed] [Google Scholar]
  26. Lazdunski A., Murgier M., Lazdunski C. Phospholipid synthesis-dependent activity of aminopeptidase N in intact cells of Escherichia coli. J Mol Biol. 1979 Feb 25;128(2):127–141. doi: 10.1016/0022-2836(79)90122-0. [DOI] [PubMed] [Google Scholar]
  27. Lueking D. R., Fraley R. T., Kaplan S. Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Fate of "old" and "new" membrane. J Biol Chem. 1978 Jan 25;253(2):451–457. [PubMed] [Google Scholar]
  28. Lutkenhaus J. F., Moore B. A., Masters M., Donachie W. D. Individual proteins are synthesized continuously throughout the Escherichia coli cell cycle. J Bacteriol. 1979 May;138(2):352–360. doi: 10.1128/jb.138.2.352-360.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Mirelman D., Yashouv-Gan Y., Nuchamovitz Y., Rozenhak S., Ron E. Z. Murein biosynthesis during a synchromous cell cycle of Escherichia coli B. J Bacteriol. 1978 May;134(2):458–461. doi: 10.1128/jb.134.2.458-461.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Mirelman D., Yashouv-Gan Y., Schwarz U. Regulation of murein biosynthesis and septum formation in filamentous cells of Escherichia coli PAT 84. J Bacteriol. 1977 Mar;129(3):1593–1600. doi: 10.1128/jb.129.3.1593-1600.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Oki M. Correlation between metabolism of phosphatidylglycerol and membrane synthesis in Escherichia coli. J Mol Biol. 1972 Jul 21;68(2):249–264. doi: 10.1016/0022-2836(72)90212-4. [DOI] [PubMed] [Google Scholar]
  32. Pierucci O. Phospholipid synthesis during the cell division cycle of Escherichia coli. J Bacteriol. 1979 May;138(2):453–460. doi: 10.1128/jb.138.2.453-460.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Raetz C. R. Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli. Microbiol Rev. 1978 Sep;42(3):614–659. doi: 10.1128/mr.42.3.614-659.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Schulman H., Kennedy E. P. Relation of turnover of membrane phospholipids to synthesis of membrane-derived oligosaccharides of Escherichia coli. J Biol Chem. 1977 Jun 25;252(12):4250–4255. [PubMed] [Google Scholar]
  35. Silbert D. F. Arrangement of fatty acyl groups in phosphatidylethanolamine from a fatty acid auxotroph of Escherichia coli. Biochemistry. 1970 Sep 1;9(18):3631–3640. doi: 10.1021/bi00820a021. [DOI] [PubMed] [Google Scholar]
  36. Silbert D. F., Ladenson R. C., Honegger J. L. The unsaturated fatty acid requirement in Escherichia coli. Temperature dependence and total replacement by branched-chain fatty acids. Biochim Biophys Acta. 1973 Jul 6;311(3):349–361. doi: 10.1016/0005-2736(73)90315-5. [DOI] [PubMed] [Google Scholar]
  37. Stárka J., Moravová J. Phospholipids and cellular division of Escherichia coli. J Gen Microbiol. 1970 Feb;60(2):251–257. doi: 10.1099/00221287-60-2-251. [DOI] [PubMed] [Google Scholar]
  38. Weppner W. A., Neuhaus F. C. Initial membrane reaction in peptidoglycan synthesis. Interaction of lipid with phospho-N-acetylmuramyl-pentapeptide translocase. Biochim Biophys Acta. 1979 Apr 19;552(3):418–427. doi: 10.1016/0005-2736(79)90186-x. [DOI] [PubMed] [Google Scholar]
  39. Zuchowski C., Pierucci O. Phospholipid turnover during the division cycle of Escherichia coli. J Bacteriol. 1978 Mar;133(3):1533–1535. doi: 10.1128/jb.133.3.1533-1535.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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