Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Jan;145(1):573–582. doi: 10.1128/jb.145.1.573-582.1981

Biological consequences of infection of Escherichia coli B by alkylated T7 bacteriophage.

B Karska-Wysocki, M D Mamet-Bratley
PMCID: PMC217307  PMID: 7007326

Abstract

Alkylation of T7 bacteriophage considerably delayed phage development and reduced the phage's killing action on host cells. Only a small fraction of infected cells produced phage. For these phages, the latent period was markedly prolonged but the burst was equivalent to or only slightly lower than that of untreated phage. In the progeny of alkylated phage, there was an increase in the fraction of defective particles as well as a change in their morphology. These data show that infection with alkylated T7 bacteriophage is to a large degree abortive; hence, biological consequences of this infection are very different from those characteristic of a normal virus infection.

Full text

PDF
573

Images in this article

580Image
on p.580

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Campbell J. L., Richardson C. C., Studier F. W. Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA. Proc Natl Acad Sci U S A. 1978 May;75(5):2276–2280. doi: 10.1073/pnas.75.5.2276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chamberlin M. Isolation and characterization of prototrophic mutants of Escherichia coli unable to support the intracellular growth of T7. J Virol. 1974 Sep;14(3):509–516. doi: 10.1128/jvi.14.3.509-516.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dreiseikelmann B., Wackernagel W. The terminal redundant regions of bacteriophage T7 DNA: their necessity for phage production studied by the infectivity of T7 DNA after modification by various exonucleases. Mol Gen Genet. 1978 Feb 27;159(3):321–328. doi: 10.1007/BF00268269. [DOI] [PubMed] [Google Scholar]
  4. Dussault P., Bourgault J. M., Verly W. G. T7 coliphage inactivation by nitrous acid. Biochim Biophys Acta. 1970 Aug 8;213(2):312–319. doi: 10.1016/0005-2787(70)90039-0. [DOI] [PubMed] [Google Scholar]
  5. Hausmann R. Bacteriophage T7 genetics. Curr Top Microbiol Immunol. 1976;75:77–110. doi: 10.1007/978-3-642-66530-1_3. [DOI] [PubMed] [Google Scholar]
  6. Karska-Wysocki B., Mamet-Bratley M. D., Przewlocki G. DNA injection and genetic recombination of alkylated bacteriophage T7 in the presence of nalidixic acid. J Virol. 1977 Nov;24(2):716–719. doi: 10.1128/jvi.24.2.716-719.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karska-Wysocki B., Mamet-Bratley M. D., Verly W. G. Action of ethyl and methyl methane sulfonates on DNA injection and genetic recombination in T7 bacteriophage. J Virol. 1976 Aug;19(2):318–324. doi: 10.1128/jvi.19.2.318-324.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Karska-Wysocki B., Thibodeau L., Verly W. G. Inactivation of the T7 coliphage by monofunctional alkylating agents. Action of phage adsorption and injection of its DNA. Biochim Biophys Acta. 1976 Jun 18;435(2):184–191. doi: 10.1016/0005-2787(76)90249-5. [DOI] [PubMed] [Google Scholar]
  9. Kerr C., Sadowski P. D. The involvement of genes 3,4,5 and 6 in genetic recombination in bacteriophage T7. Virology. 1975 May;65(1):281–285. doi: 10.1016/0042-6822(75)90031-8. [DOI] [PubMed] [Google Scholar]
  10. Lane D., Mamet-Bratley M. D., Karska-Wysocki B. Host-cell reactivation of alkylated T7 bacteriophage. Biochim Biophys Acta. 1979 Oct 25;564(3):495–506. doi: 10.1016/0005-2787(79)90039-x. [DOI] [PubMed] [Google Scholar]
  11. Lawley P. D., Lethbridge J. H., Edwards P. A., Shooter K. V. Inactivation of bacteriophage T7 by mono- and difunctional sulphur mustards in relation to cross-linking and depurination of bacteriophage DNA. J Mol Biol. 1969 Jan 14;39(1):181–198. doi: 10.1016/0022-2836(69)90341-6. [DOI] [PubMed] [Google Scholar]
  12. Luria S. E. A Growth-Delaying Effect of Ultraviolet Radiation. Proc Natl Acad Sci U S A. 1944 Dec 15;30(12):393–397. doi: 10.1073/pnas.30.12.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mukai F., Streisinger G., Miller B. The mechanism of lysis in phage T4-infected cells. Virology. 1967 Nov;33(3):398–404. doi: 10.1016/0042-6822(67)90115-8. [DOI] [PubMed] [Google Scholar]
  14. Pao C. C., Speyer J. F. Order of injection of T7 bacteriophage DNA. J Virol. 1973 Jun;11(6):1024–1026. doi: 10.1128/jvi.11.6.1024-1026.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ponta H., Altendorf K. H., Schweiger M., Hirsch-Kaufmann M., Pfennig-Yeh M. L., Herrlich P. E. coli membranes become permeable to ions following T7-virus-infection. Mol Gen Genet. 1976 Dec 8;149(2):145–150. doi: 10.1007/BF00332882. [DOI] [PubMed] [Google Scholar]
  16. Ponta H., Orätzel M., Pfennig-Yeh M., Hirsch-Kauffmann M., Schweiger M. Membrane alteration induced by T7 virus infection. FEBS Lett. 1977 Feb 1;73(2):207–209. doi: 10.1016/0014-5793(77)80982-4. [DOI] [PubMed] [Google Scholar]
  17. Radman M., Cordone L., Krsmanovic-Simic D., Errera M. Complementary action of recombination and excision in the repair of ultraviolet irradiation damage to DNA. J Mol Biol. 1970 Apr 14;49(1):203–212. doi: 10.1016/0022-2836(70)90386-4. [DOI] [PubMed] [Google Scholar]
  18. Ray U., Bartenstein L., Drake J. W. Inactivation of bacteriophage T4 by ethyl methanesulfonate: influence of host and viral genotypes. J Virol. 1972 Mar;9(3):440–447. doi: 10.1128/jvi.9.3.440-447.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roeder G. S., Sadowski P. D. Bacteriophage T7 morphogenesis: phage-related particles in cells infected with wild-type and mutant T7 phage. Virology. 1977 Jan;76(1):263–285. doi: 10.1016/0042-6822(77)90302-6. [DOI] [PubMed] [Google Scholar]
  20. Silberstein S., Inouye M. Studies on the role of bacteriophage T7 lysozyme during phage infection. J Mol Biol. 1975 Jul 25;96(1):1–11. doi: 10.1016/0022-2836(75)90178-3. [DOI] [PubMed] [Google Scholar]
  21. Studier F. W. Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host. J Mol Biol. 1975 May 15;94(2):283–295. doi: 10.1016/0022-2836(75)90083-2. [DOI] [PubMed] [Google Scholar]
  22. Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]
  23. Verly W. G., Brakier L. The letal action of monofunctional and bifunctional alkylating agents on T7 coliphage. Biochim Biophys Acta. 1969 Feb 18;174(2):674–685. doi: 10.1016/0005-2787(69)90296-2. [DOI] [PubMed] [Google Scholar]
  24. Verly W. G., Crine P., Bannon P., Forget A. Immediate inactivation of T7 coliphage treated by monofunctional alkylating agents. Biochim Biophys Acta. 1974 May 17;349(2):204–213. doi: 10.1016/0005-2787(74)90081-1. [DOI] [PubMed] [Google Scholar]
  25. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES