Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Jan;145(1):641–643. doi: 10.1128/jb.145.1.641-643.1981

Mechanism of the rel defect in beta-galactosidase synthesis.

D Foley, P Dennis, J Gallant
PMCID: PMC217317  PMID: 6780525

Abstract

Relaxed (relA) mutants of Escherichia coli are defective in beta-galactosidase synthesis during amino acid limitation. We show here that this defect comprises both a transcriptional component and a translational component.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aboud M., Pastan I. Stimulation of lac transcription by guanosine 5'-diphosphate 2'(or 3')-diphosphate and transfer ribonucleic acid. J Biol Chem. 1973 May 10;248(9):3356–3358. [PubMed] [Google Scholar]
  2. Cashel M. Regulation of bacterial ppGpp and pppGpp. Annu Rev Microbiol. 1975;29:301–318. doi: 10.1146/annurev.mi.29.100175.001505. [DOI] [PubMed] [Google Scholar]
  3. De Crombrugghe B., Chen B., Gottesman M., Pastan I., Varmus H. E., Emmer M., Perlman R. L. Regulation of lac mRNA synthesis in a soluble cell-free system. Nat New Biol. 1971 Mar 10;230(10):37–40. doi: 10.1038/newbio230037a0. [DOI] [PubMed] [Google Scholar]
  4. Dennis P. P., Nomura M. Regulation of the expression of ribosomal protein genes in Escherichia coli. J Mol Biol. 1975 Sep 5;97(1):61–76. doi: 10.1016/s0022-2836(75)80022-2. [DOI] [PubMed] [Google Scholar]
  5. Edelmann P., Gallant J. Mistranslation in E. coli. Cell. 1977 Jan;10(1):131–137. doi: 10.1016/0092-8674(77)90147-7. [DOI] [PubMed] [Google Scholar]
  6. Ennis H. L., Kievitt K. D., Artman M. Synthesis of beta-galactosidase messenger RNA during amino acid starvation of stringent and relaxed strains of Escherichia coli. Biochem Biophys Res Commun. 1974 Jul 10;59(1):429–436. doi: 10.1016/s0006-291x(74)80224-x. [DOI] [PubMed] [Google Scholar]
  7. Gallant J. A. Stringent control in E. coli. Annu Rev Genet. 1979;13:393–415. doi: 10.1146/annurev.ge.13.120179.002141. [DOI] [PubMed] [Google Scholar]
  8. HARTWELL L. H., MAGASANIK B. THE MOLECULAR BASIS OF HISTIDASE INDUCTION IN BACILLUS SUBTILIS. J Mol Biol. 1963 Oct;7:401–420. doi: 10.1016/s0022-2836(63)80033-9. [DOI] [PubMed] [Google Scholar]
  9. Hall B., Gallant J. Defective translation in RC - cells. Nat New Biol. 1972 May 31;237(74):131–135. doi: 10.1038/newbio237131a0. [DOI] [PubMed] [Google Scholar]
  10. Morris D. W., Kjeldgaard N. O. Evidence for the non-co-ordinate regulation of ribonucleic acid synthesis in stringent strains of Escherichia coli. J Mol Biol. 1968 Jan 14;31(1):145–148. doi: 10.1016/0022-2836(68)90064-8. [DOI] [PubMed] [Google Scholar]
  11. Nierlich D. P. Regulation of bacterial growth, RNA, and protein synthesis. Annu Rev Microbiol. 1978;32:393–432. doi: 10.1146/annurev.mi.32.100178.002141. [DOI] [PubMed] [Google Scholar]
  12. O'Farrell P. H. The suppression of defective translation by ppGpp and its role in the stringent response. Cell. 1978 Jul;14(3):545–557. doi: 10.1016/0092-8674(78)90241-6. [DOI] [PubMed] [Google Scholar]
  13. Parker J., Friesen J. D. "Two out of three" codon reading leading to mistranslation in vivo. Mol Gen Genet. 1980 Feb;177(3):439–445. doi: 10.1007/BF00271482. [DOI] [PubMed] [Google Scholar]
  14. Primakoff P. In vivo role of the relA+ gene in regulation of the lac operon. J Bacteriol. 1981 Jan;145(1):410–416. doi: 10.1128/jb.145.1.410-416.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yang H. L., Zubay G., Urm E., Heiness G., Cashel M. Effects of guanosine tetraphosphate, guanosine pentaphosphate, and beta-gamma methylenyl-guanosine pentaphosphate on gene expression of Escherichia coli in vitro. Proc Natl Acad Sci U S A. 1974 Jan;71(1):63–67. doi: 10.1073/pnas.71.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES