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. 1983 Jan;153(1):21–26. doi: 10.1128/jb.153.1.21-26.1983

Assembly In Vivo of Enterotoxin from Escherichia coli: Formation of the B Subunit Oligomer

Timothy R Hirst 1,, Simon J S Hardy 1,, Linda L Randall 1
PMCID: PMC217337  PMID: 6336733

Abstract

An oligomer of the B subunit of heat-labile enterotoxin of Escherichia coli has been observed in minicells and in whole cells. There is a delay after synthesis of the B subunit before it appears in the oligomer. The delay is not due to slow processing of the precursor. A similar delay in oligomerization of the major outer membrane protein OmpF is also described.

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Selected References

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  1. Crowlesmith I., Gamon K., Henning U. Precursor proteins are intermediates in vivo in the synthesis of two major outer membrane proteins, the OmpA and OmpF proteins, of Escherichia coli K12. Eur J Biochem. 1981 Jan;113(2):375–380. doi: 10.1111/j.1432-1033.1981.tb05076.x. [DOI] [PubMed] [Google Scholar]
  2. Dallas W. S., Falkow S. Amino acid sequence homology between cholera toxin and Escherichia coli heat-labile toxin. Nature. 1980 Dec 4;288(5790):499–501. doi: 10.1038/288499a0. [DOI] [PubMed] [Google Scholar]
  3. Dallas W. S., Gill D. M., Falkow S. Cistrons encoding Escherichia coli heat-labile toxin. J Bacteriol. 1979 Sep;139(3):850–858. doi: 10.1128/jb.139.3.850-858.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Enequist H. G., Hirst T. R., Harayama S., Hardy S. J., Randall L. L. Energy is required for maturation of exported proteins in Escherichia coli. Eur J Biochem. 1981 May 15;116(2):227–233. doi: 10.1111/j.1432-1033.1981.tb05323.x. [DOI] [PubMed] [Google Scholar]
  5. Gill D. M., Clements J. D., Robertson D. C., Finkelstein R. A. Subunit number and arrangement in Escherichia coli heat-labile enterotoxin. Infect Immun. 1981 Sep;33(3):677–682. doi: 10.1128/iai.33.3.677-682.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Henning U., Schmidmayr W., Hindennach I. Major proteins of the outer cell envelope membrane of Escherichia coli K-12: multiple species of protein I. Mol Gen Genet. 1977 Sep 9;154(3):293–298. doi: 10.1007/BF00571285. [DOI] [PubMed] [Google Scholar]
  7. Lai C. Y., Cancedda F., Chang D. Primary structure of cholera toxin subunit A1: isolation, partial sequences and alignment of the BrCN fragments. FEBS Lett. 1979 Apr 1;100(1):85–89. doi: 10.1016/0014-5793(79)81136-9. [DOI] [PubMed] [Google Scholar]
  8. Lai C. Y. Determination of the primary structure of cholera toxin B subunit. J Biol Chem. 1977 Oct 25;252(20):7249–7256. [PubMed] [Google Scholar]
  9. Levy S. B. Very stable prokaryotic messenger RNA in chromosomeless Escherichia coli minicells. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2900–2904. doi: 10.1073/pnas.72.8.2900. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Palva E. T., Hirst T. R., Hardy S. J., Holmgren J., Randall L. Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli. J Bacteriol. 1981 Apr;146(1):325–330. doi: 10.1128/jb.146.1.325-330.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Randall L. L., Hardy S. J., Josefsson L. G. Precursors of three exported proteins in Escherichia coli. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1209–1212. doi: 10.1073/pnas.75.3.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Randall L. L., Hardy S. J. Synthesis of exported proteins by membrane-bound polysomes from Escherichia coli. Eur J Biochem. 1977 May 2;75(1):43–53. doi: 10.1111/j.1432-1033.1977.tb11502.x. [DOI] [PubMed] [Google Scholar]
  13. Spicer E. K., Kavanaugh W. M., Dallas W. S., Falkow S., Konigsberg W. H., Schafer D. E. Sequence homologies between A subunits of Escherichia coli and Vibrio cholerae enterotoxins. Proc Natl Acad Sci U S A. 1981 Jan;78(1):50–54. doi: 10.1073/pnas.78.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wensink J., Gankema H., Jansen W. H., Guinée P. A., Witholt B. Isolation of the membranes of an enterotoxigenic strain of Escherichia coli and distribution of enterotoxin activity in different subcellular fractions. Biochim Biophys Acta. 1978 Dec 4;514(1):128–136. doi: 10.1016/0005-2736(78)90082-2. [DOI] [PubMed] [Google Scholar]
  15. Yu F., Ichihara S., Mizushima S. A major outer membrane protein (O-8) of Escherichia coli K-12 exists as a trimer in sodium dodecyl sulfate solution. FEBS Lett. 1979 Apr 1;100(1):71–74. doi: 10.1016/0014-5793(79)81133-3. [DOI] [PubMed] [Google Scholar]

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