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. 1983 Jan;153(1):100–108. doi: 10.1128/jb.153.1.100-108.1983

Formation of oligomeric structures from plasmid DNA carrying cos lambda that is packaged into bacteriophage lambda heads.

T Miwa, K Matsubara
PMCID: PMC217346  PMID: 6217189

Abstract

Plasmids that carry cos lambda, the region necessary for lambda phage packaging and that are as small as four kilobases in size can be packaged into lambda phage heads in head-to-tail tandem oligomeric structures. Multimeric oligomers as large as undecamers have been detected. Oligomer formation depends upon the products of red and gam of lambda, and the general recombination occurs between different plasmids that share homologous DNA regions. The packaging efficiency of plasmids depends on its copy number in cells and its genome size. Upon injection into a cell, the DNA establishes itself as a plasmid in a tandem structure. When such a plasmid in a high oligomeric structure is used as the source of packaging DNA, the packaging efficiency of the plasmids is elevated. The oligomers are stable in recA cells, whereas they drift toward lower oligomers in recA+ cells.

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Selected References

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  1. Akaboshi E., Matsubara K. Protein synthesis induced by infection with packaged lambda dv plasmid. Plasmid. 1981 Nov;6(3):315–324. doi: 10.1016/0147-619x(81)90039-1. [DOI] [PubMed] [Google Scholar]
  2. Blattner F. R., Blechl A. E., Denniston-Thompson K., Faber H. E., Richards J. E., Slightom J. L., Tucker P. W., Smithies O. Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections. Science. 1978 Dec 22;202(4374):1279–1284. doi: 10.1126/science.725603. [DOI] [PubMed] [Google Scholar]
  3. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins J., Hohn B. Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4242–4246. doi: 10.1073/pnas.75.9.4242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Enquist L. W., Skalka A. Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. I. Interaction of red, gam and rec. J Mol Biol. 1973 Apr 5;75(2):185–212. doi: 10.1016/0022-2836(73)90016-8. [DOI] [PubMed] [Google Scholar]
  6. Feiss M., Fisher R. A., Crayton M. A., Egner C. Packaging of the bacteriophage lambda chromosome: effect of chromosome length. Virology. 1977 Mar;77(1):281–293. doi: 10.1016/0042-6822(77)90425-1. [DOI] [PubMed] [Google Scholar]
  7. Feiss M., Siegele D. A. Packaging of the bacteriophage lambda chromosome: dependence of cos cleavage on chromosome length. Virology. 1979 Jan 15;92(1):190–200. doi: 10.1016/0042-6822(79)90224-1. [DOI] [PubMed] [Google Scholar]
  8. Feiss M., Siegele D. A., Rudolph C. F., Frackman S. Cosmid DNA packaging in vivo. Gene. 1982 Feb;17(2):123–130. doi: 10.1016/0378-1119(82)90064-6. [DOI] [PubMed] [Google Scholar]
  9. Hayakawa Y., Matsubara K. Construction and some properties of packageable plasmid F. Mol Gen Genet. 1979 Jan 16;169(1):107–112. doi: 10.1007/BF00267551. [DOI] [PubMed] [Google Scholar]
  10. Hobom G., Hogness D. S. The role of recombination in the formation of circular oligomers of the lambda plasmid. J Mol Biol. 1974 Sep 5;88(1):65–87. doi: 10.1016/0022-2836(74)90295-2. [DOI] [PubMed] [Google Scholar]
  11. Kahn M., Helinski D. R. Construction of a novel plasmid-phage hybrid: use of the hybrid to demonstrate ColE1 DNA replication in vivo in the absence of a ColE1-specified protein. Proc Natl Acad Sci U S A. 1978 May;75(5):2200–2204. doi: 10.1073/pnas.75.5.2200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mukai T., Matsubara K., Takagi Y. Cloning bacterial genes with plasmid gammadv. Mol Gen Genet. 1976 Aug 2;146(3):269–274. doi: 10.1007/BF00701250. [DOI] [PubMed] [Google Scholar]
  13. Szybalski E. H., Szybalski W. A comprehensive molecular map of bacteriophage lambda. Gene. 1979 Nov;7(3-4):217–270. doi: 10.1016/0378-1119(79)90047-7. [DOI] [PubMed] [Google Scholar]
  14. Timmis K., Cabello F., Cohen S. N. Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242–2246. doi: 10.1073/pnas.72.6.2242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Umene K., Shimada K., Takagi Y. Packaging of ColE1 DNA having a lambda phage cohesive end site. Mol Gen Genet. 1978 Feb 7;159(1):39–45. doi: 10.1007/BF00401746. [DOI] [PubMed] [Google Scholar]
  16. Vollenweider H. J., Fiandt M., Rosenvold E. C., Szybalski W. Packaging of plasmid DNA containing the cohesive ends of coliphage lambda. Gene. 1980 Apr;9(1-2):171–174. doi: 10.1016/0378-1119(80)90174-2. [DOI] [PubMed] [Google Scholar]
  17. Yamagishi H., Inokuchi H., Ozeki H. Excision and duplication of su3+-transducing fragments carried by bacteriophage phi 80. I. Novel structure of phi 80sus2psu3+ DNA molecule. J Virol. 1976 Jun;18(3):1016–1023. doi: 10.1128/jvi.18.3.1016-1023.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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