Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Jan;153(1):310–315. doi: 10.1128/jb.153.1.310-315.1983

Carbonyl cyanide-m-chlorophenyl hydrazone-resistant Escherichia coli mutant that exhibits a temperature-sensitive unc phenotype.

M Ito, Y Ohnishi, S Itoh, M Nishimura
PMCID: PMC217372  PMID: 6217194

Abstract

Two spontaneous Escherichia coli mutant strains which are resistant to an oxidative phosphorylation uncoupler, carbonyl cyanide-m-chlorophenyl hydrazone, were isolated. Strain CM22 (ccr-2) was resistant to another uncoupler, pentachlorophenol, and to the inhibitors of proton-translocating ATPase, namely tributyltin and sodium azide. Carbonyl cyanide-m-chlorophenyl hydrazone or pentachlorophenol administered to cell suspensions of strain CM22 did not cause a pH change induced by H+ influx, and a similar result was obtained with everted particles. The respiratory rate of strain CM22 with succinate was twice that of wild-type strain KH434. When carbonyl cyanide-m-chlorophenyl hydrazone was administered, a stimulation of O2 uptake was observed in wild-type strain KH434 but not in the mutant strain CM22. Strain CM22 did not grow on succinate at 42 degrees C. Isolation of a true revertant at a frequency of 10(-8) demonstrated that the pleiotropic phenotype was induced by a single mutation. P1 transduction indicated that the mutant allele, ccr-2, was cotransduced with the ilv genes at a frequency of about 55%.

Full text

PDF
310

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burstein C., Tiankova L., Kepes A. Respiratory control in Escherichia coli K 12. Eur J Biochem. 1979 Mar;94(2):387–392. doi: 10.1111/j.1432-1033.1979.tb12905.x. [DOI] [PubMed] [Google Scholar]
  3. Cox G. B., Gibson F. Studies on electron transport and energy-linked reactions using mutants of Escherichia coli. Biochim Biophys Acta. 1974 Apr 30;346(1):1–25. doi: 10.1016/0304-4173(74)90010-x. [DOI] [PubMed] [Google Scholar]
  4. Cyboron G. W., Dryer R. L. Uncoupling of hamster brown adipose and liver mitochondria by 2-azido-4-nitrophenol and binding properties of the reagent. Arch Biochem Biophys. 1977 Feb;179(1):141–146. doi: 10.1016/0003-9861(77)90097-2. [DOI] [PubMed] [Google Scholar]
  5. Decker S. J., Lang D. R. Membrane bioenergetic parameters in uncoupler-resistant mutants of Bacillus megaterium. J Biol Chem. 1978 Oct 10;253(19):6738–6743. [PubMed] [Google Scholar]
  6. Decker S. J., Lang D. R. Mutants of Bacillus megaterium resistant to uncouplers of oxidative phosphorylation. J Biol Chem. 1977 Sep 10;252(17):5936–5938. [PubMed] [Google Scholar]
  7. Downie J. A., Gibson F., Cox G. B. Membrane adenosine triphosphatases of prokaryotic cells. Annu Rev Biochem. 1979;48:103–131. doi: 10.1146/annurev.bi.48.070179.000535. [DOI] [PubMed] [Google Scholar]
  8. Hanstein W. G., Hatefi Y. Characterization and localization of mitochondrial uncoupler binding sites with an uncoupler capable of photoaffinity labeling. J Biol Chem. 1974 Mar 10;249(5):1356–1362. [PubMed] [Google Scholar]
  9. Hanstein W. G. Uncoupling of oxidative phosphorylation. Biochim Biophys Acta. 1976 Sep 27;456(2):129–148. doi: 10.1016/0304-4173(76)90010-0. [DOI] [PubMed] [Google Scholar]
  10. Ito M., Ohnishi Y. Isolation of Escherichia coli mutants which are resistant to an inhibitor of H+-ATPase, tributyltin and also to uncouplers of oxidative phosphorylation. FEBS Lett. 1981 Dec 28;136(2):225–230. doi: 10.1016/0014-5793(81)80623-0. [DOI] [PubMed] [Google Scholar]
  11. Kagawa Y., Sone N., Hirata H., Yoshida M. Structure and function of H+-ATPase. J Bioenerg Biomembr. 1979 Aug;11(3-4):39–78. doi: 10.1007/BF00743196. [DOI] [PubMed] [Google Scholar]
  12. Kovác L. Biochemical mutants: an approach to mitochondrial energy coupling. Biochim Biophys Acta. 1974 Oct 31;346(2):101–135. [PubMed] [Google Scholar]
  13. Mitchell P. Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc. 1966 Aug;41(3):445–502. doi: 10.1111/j.1469-185x.1966.tb01501.x. [DOI] [PubMed] [Google Scholar]
  14. Nelson N. Structure and function of chloroplast ATPase. Biochim Biophys Acta. 1976 Nov 30;456(3-4):314–338. doi: 10.1016/0304-4173(76)90003-3. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES