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. 1983 Jan;153(1):345–349. doi: 10.1128/jb.153.1.345-349.1983

Isolation of Saccharomyces cerevisiae TRP3.

J L Paluh, H Zalkin
PMCID: PMC217377  PMID: 6294052

Abstract

Several plasmids, isolated from two plasmid pools, complemented a Saccharomyces cerevisiae trp3 mutant with defective indole-3-glycerol-phosphate synthase activity. Restriction mapping indicated that a 1.2-kilobase StuI segment was common to all complementing plasmids. Southern blot hybridization established that a cloned 5.2-kilobase BamHI fragment was derived intact from chromosomal DNA. A yeast trp3 mutant transformed with trp3-complementing plasmids contained approximately 40-fold elevated indole-3-glycerol-phosphate synthase activity. These plasmids also complemented an Escherichia coli trpC mutant, and transformants exhibited enzyme activity. Yeast trp3 is therefore associated with a 1.2-kilobase StuI DNA segment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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