Abstract
A chemically defined medium (CC-494) was used to study the nutritional requirements of three spiroplasmas representing three distinct serogroups: flower spiroplasmas [Spiroplasma floricola and FS (SR-3)] and honeybee spiroplasma [HBS (AS-576)]. Glucose, fructose, and mannose were utilized by all three spiroplasmas. In addition, the honeybee spiroplasma could ferment trehalose, FS (SR-3) could ferment sucrose, and S. floricola could ferment trehalose, sucrose, and raffinose. The three spiroplasmas varied greatly in their requirements of amino acids for growth. S. floricola was the only strain that utilized arginine. HBS (AS-576) required at least one purine and one pyrimidine base (either free base or ribonucleoside) for growth, while both flower spiroplasmas grew with only one base in the medium. Oleic acid, cholesterol, and bovine serum albumin were essential to all three spiroplasmas. Palmitic acid, which was nonessential, promoted growth significantly.
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Selected References
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- Chang C. J., Chen T. A. Spiroplasmas: cultivation in chemically defined medium. Science. 1982 Feb 26;215(4536):1121–1122. doi: 10.1126/science.7063844. [DOI] [PubMed] [Google Scholar]
- Davis R. E., Lee I. M., Basciano L. K. Spiroplasmas: serological grouping of strains associated with plants and insects. Can J Microbiol. 1979 Aug;25(8):861–866. doi: 10.1139/m79-128. [DOI] [PubMed] [Google Scholar]
- Davis R. E. Spiroplasma associated with flowers of the tulip tree (Liriodendron tulipifera L.). Can J Microbiol. 1978 Aug;24(8):954–959. doi: 10.1139/m78-158. [DOI] [PubMed] [Google Scholar]
- Igwegbe E. C., Stevens C., Hollis J. J., Jr An in vitro comparison of some biochemical and biological properties of California and Morocco isolates of Spiroplasma citri. Can J Microbiol. 1979 Oct;25(10):1125–1132. doi: 10.1139/m79-175. [DOI] [PubMed] [Google Scholar]
- Mitchell A., Finch L. R. Pathways of nucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides. J Bacteriol. 1977 Jun;130(3):1047–1054. doi: 10.1128/jb.130.3.1047-1054.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mudd J. B., Ittig M., Roy B., Latrille J., Bové J. M. Composition and enzyme activities of Spiroplasma citri membranes. J Bacteriol. 1977 Mar;129(3):1250–1256. doi: 10.1128/jb.129.3.1250-1256.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Razin S. The mycoplasmas. Microbiol Rev. 1978 Jun;42(2):414–470. doi: 10.1128/mr.42.2.414-470.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TOURTELLOTTE M. E., MOROWITZ H. J., KASIMER P. DEFINED MEDIUM FOR MYCOPLASMA LAIDLAWII. J Bacteriol. 1964 Jul;88:11–15. doi: 10.1128/jb.88.1.11-15.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Townsend R. Arginine metabolism by spiroplasma citri. J Gen Microbiol. 1976 Jun;94(2):417–420. doi: 10.1099/00221287-94-2-417. [DOI] [PubMed] [Google Scholar]
- Whitcomb R. F. The genus Spiroplasma. Annu Rev Microbiol. 1980;34:677–709. doi: 10.1146/annurev.mi.34.100180.003333. [DOI] [PubMed] [Google Scholar]