Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Jan;153(1):566–570. doi: 10.1128/jb.153.1.566-570.1983

CFA/I-ST plasmids: comparison of enterotoxigenic Escherichia coli (ETEC) of serogroups O25, O63, O78, and O128 and mobilization from an R factor-containing epidemic ETEC isolate.

B E Murray, D J Evans Jr, M E Penãranda, D G Evans
PMCID: PMC217412  PMID: 6336743

Abstract

Colonization factor antigen I (CFA/I) plays an important role in the pathogenesis of diarrhea due to enterotoxigenic Escherichia coli. In this study, we examined 11 CFA/I+ enterotoxigenic E. coli from serogroups O25, O63, O78, and O128 and found that with all strains, spontaneous loss of CFA/I was associated with the loss of heat-stable toxin (ST) and with the loss of a single plasmid ranging in size from 54 to 60 megadaltons; when heat-labile toxin was lost, this was associated with the loss of another plasmid. The R factor of one strain, TX432 (O78:H12:CFA/I+; ST+), was found to mobilize the CFA/I-ST plasmid into E. coli K-12 at a frequency of 20%. These studies provide further evidence that CFA/I production is plasmid mediated in enterotoxigenic E. coli belonging to serogroups O25, O63, O78, and O128.

Full text

PDF
566

Images in this article

568Image
on p.568

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black R. E., Merson M. H., Rowe B., Taylor P. R., Abdul Alim A. R., Gross R. J., Sack D. A. Enterotoxigenic Escherichia coli diarrhoea: acquired immunity and transmission in an endemic area. Bull World Health Organ. 1981;59(2):263–268. [PMC free article] [PubMed] [Google Scholar]
  2. Dallas W. S., Gill D. M., Falkow S. Cistrons encoding Escherichia coli heat-labile toxin. J Bacteriol. 1979 Sep;139(3):850–858. doi: 10.1128/jb.139.3.850-858.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  4. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  5. Elwell L. P., Shipley P. L. Plasmid-mediated factors associated with virulence of bacteria to animals. Annu Rev Microbiol. 1980;34:465–496. doi: 10.1146/annurev.mi.34.100180.002341. [DOI] [PubMed] [Google Scholar]
  6. Evans D. G., Evans D. J., Jr, Clegg S., Pauley J. A. Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infect Immun. 1979 Aug;25(2):738–748. doi: 10.1128/iai.25.2.738-748.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Evans D. G., Evans D. J., Jr New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups O6 and O8. Infect Immun. 1978 Aug;21(2):638–647. doi: 10.1128/iai.21.2.638-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans D. G., Evans D. J., Jr, Tjoa W. S., DuPont H. L. Detection and characterization of colonization factor of enterotoxigenic Escherichia coli isolated from adults with diarrhea. Infect Immun. 1978 Feb;19(2):727–736. doi: 10.1128/iai.19.2.727-736.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Evans D. G., Evans D. J., Jr, Tjoa W. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infect Immun. 1977 Nov;18(2):330–337. doi: 10.1128/iai.18.2.330-337.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Evans D. G., Silver R. P., Evans D. J., Jr, Chase D. G., Gorbach S. L. Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans. Infect Immun. 1975 Sep;12(3):656–667. doi: 10.1128/iai.12.3.656-667.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Evans D. J., Jr, Evans D. G., DuPont H. L., Orskov F., Orskov I. Patterns of loss of enterotoxigenicity by Escherichia coli isolated from adults with diarrhea: suggestive evidence for an interrelationship with serotype. Infect Immun. 1977 Jul;17(1):105–111. doi: 10.1128/iai.17.1.105-111.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Evans D. J., Jr, Evans D. G., Young L. S., Pitt J. Hemagglutination typing of Escherichia coli: definition of seven hemagglutination types. J Clin Microbiol. 1980 Aug;12(2):235–242. doi: 10.1128/jcm.12.2.235-242.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Luke R. K., Ryan N. J., Barnes G. L., Bishop R. F., Dayaratne R. D. Enterotoxin-producing Escherichia coli and diarrhoea among newborn babies in Darwin. Med J Aust. 1978 Feb 11;1(3):170–171. doi: 10.5694/j.1326-5377.1978.tb107817.x. [DOI] [PubMed] [Google Scholar]
  14. McConnell M. M., Smith H. R., Willshaw G. A., Field A. M., Rowe B. Plasmids coding for colonization factor antigen I and heat-stable enterotoxin production isolated from enterotoxigenic Escherichia coli: comparison of their properties. Infect Immun. 1981 May;32(2):927–936. doi: 10.1128/iai.32.2.927-936.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McHugh G. L., Swartz M. N. Elimination of plasmids from several bacterial species by novobiocin. Antimicrob Agents Chemother. 1977 Sep;12(3):423–426. doi: 10.1128/aac.12.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Merson M. H., Morris G. K., Sack D. A., Wells J. G., Feeley J. C., Sack R. B., Creech W. B., Kapikian A. Z., Gangarosa E. J. Travelers' diarrhea in Mexico. A prospective study of physicians and family members attending a congress. N Engl J Med. 1976 Jun 10;294(24):1299–1305. doi: 10.1056/NEJM197606102942401. [DOI] [PubMed] [Google Scholar]
  17. Merson M. H., Orskov F., Orskov I., Sack R. B., Huq I., Koster F. T. Relationship between enterotoxin production and serotype in enterotoxigenic Escherichia coli. Infect Immun. 1979 Feb;23(2):325–329. doi: 10.1128/iai.23.2.325-329.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Merson M. H., Sack R. B., Islam S., Saklayen G., Huda N., Huq I., Zulich A. W., Yolken R. H., Kapikian A. Z. Disease due to enterotoxigenic Escherichia coli in Bangladeshi adults: clinical aspects and a controlled trial of tetracycline. J Infect Dis. 1980 Jun;141(6):702–711. doi: 10.1093/infdis/141.6.702. [DOI] [PubMed] [Google Scholar]
  19. Moon H. W., Nagy B., Isaacson R. E., Orskov I. Occurrence of K99 antigen on Escherichia coli isolated from pigs and colonization of pig ileum by K99+ enterotoxigenic E. coli from calves and pigs. Infect Immun. 1977 Feb;15(2):614–620. doi: 10.1128/iai.15.2.614-620.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Orskov I., Orskov F., Smith H. W., Sojka W. J. The establishment of K99, a thermolabile, transmissible escherichia coli K antigen, previously called "Kco", possessed by calf and lamb enteropathogenic strains. Acta Pathol Microbiol Scand B. 1975 Feb;83(1):31–36. doi: 10.1111/j.1699-0463.1975.tb00066.x. [DOI] [PubMed] [Google Scholar]
  21. Reis M. H., Heloiza M., Affonso T., Trabulsi L. R., Mazaitis A. J., Maas R., Maas W. K. Transfer of a CFA/I-ST plasmid promoted by a conjugative plasmid in a strain of Escherichia coli of serotype O128ac:H12. Infect Immun. 1980 Jul;29(1):140–143. doi: 10.1128/iai.29.1.140-143.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rosenberg M. L., Koplan J. P., Wachsmuth I. K., Wells J. G., Gangarosa E. J., Guerrant R. L., Sack D. A. Epidemic diarrhea at Crater Lake from enterotoxigenic Escherichia coli. A large waterborne outbreak. Ann Intern Med. 1977 Jun;86(6):714–718. doi: 10.7326/0003-4819-86-6-714. [DOI] [PubMed] [Google Scholar]
  23. Rowe B., Gross R. J., Scotland S. M., Wright A. E., Shillom G. N., Hunter N. J. Outbreak of infantile enteritis caused by enterotoxigenic Escherichia coli O6.H16. J Clin Pathol. 1978 Mar;31(3):217–219. doi: 10.1136/jcp.31.3.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ryder R. W., Wachsmuth I. K., Buxton A. E., Evans D. G., DuPont H. L., Mason E., Barrett F. F. Infantile diarrhea produced by heat-stable enterotoxigenic Escherichia coli. N Engl J Med. 1976 Oct 14;295(16):849–853. doi: 10.1056/NEJM197610142951601. [DOI] [PubMed] [Google Scholar]
  25. Sack D. A., Kaminsky D. C., Sack R. B., Wamola I. A., Orskov F., Orskov I., Slack R. C., Arthur R. R., Kapikian A. Z. Enterotoxigenic Escherichia coli diarrhea of travelers: a prospective study of American Peace Corps volunteers. Johns Hopkins Med J. 1977 Aug;141(2):63–70. [PubMed] [Google Scholar]
  26. Smith H. W., Linggood M. A. Observations on the pathogenic properties of the K88, Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhoea. J Med Microbiol. 1971 Nov;4(4):467–485. doi: 10.1099/00222615-4-4-467. [DOI] [PubMed] [Google Scholar]
  27. Wachsmuth I. K., Falkow S., Ryder R. W. Plasmid-mediated properties of a heat-stable enterotoxin-producing Escherichia coli associated with infantile diarrhea. Infect Immun. 1976 Aug;14(2):403–407. doi: 10.1128/iai.14.2.403-407.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES