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. 1983 Apr;154(1):139–145. doi: 10.1128/jb.154.1.139-145.1983

Hydrophobic interactions of group A streptococci with hexadecane droplets.

I Ofek, E Whitnack, E H Beachey
PMCID: PMC217440  PMID: 6403501

Abstract

The adherence of Streptococcus pyogenes cells to hexadecane droplets was measured by vortexing water suspensions of streptococci with hexadecane. It was found that adherence of the organisms to hexadecane droplets was abolished by pretreating the organisms with trypsin, pepsin at pH 4.5, or HCl solutions at 95 degrees C. Streptococcal adherence was best expressed in organisms harvested during the stationary phase of growth and was inhibited by fatty acid-free albumin because of the interaction of the protein with the streptococcal surfaces. The data suggest that adherence to hexadecane droplets measures the availability on the surface of S. pyogenes cells of lipophilic residues that are either hydrophobic regions of surface protein structures or, more likely, glycolipids complexed with and oriented by surface proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrett T. W. Mechanoelectrical transduction in hyaluronic acid salt solution is an entropy-driven process. Physiol Chem Phys. 1976;8(2):125–130. [PubMed] [Google Scholar]
  2. Bartelt M. A., Duncan J. L. Adherence of group A streptococci to human epithelial cells. Infect Immun. 1978 Apr;20(1):200–208. doi: 10.1128/iai.20.1.200-208.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beachey E. H., Ofek I. Epithelial cell binding of group A streptococci by lipoteichoic acid on fimbriae denuded of M protein. J Exp Med. 1976 Apr 1;143(4):759–771. doi: 10.1084/jem.143.4.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. HILL M. J., JAMES A. M., MAXTED W. R. SOME PHYSICAL INVESTIGATIONS OF THE BEHAVIOUR OF BACTERIAL SURFACES. X. THE OCCURRENCE OF LIPID IN THE STREPTOCOCCAL CELL WALL. Biochim Biophys Acta. 1963 Nov 29;75:414–424. doi: 10.1016/0006-3002(63)90629-2. [DOI] [PubMed] [Google Scholar]
  6. HILL M. J., JAMES A. M., MAXTED W. R. Some physical investigations of the behaviour of bacterial surfaces. VIII. Studies on the capsular material of Streptococcus pyogenes. Biochim Biophys Acta. 1963 Mar 19;66:264–274. doi: 10.1016/0006-3002(63)91193-4. [DOI] [PubMed] [Google Scholar]
  7. Kronvall G., Simmons A., Myhre E. B., Jonsson S. Specific absorption of human serum albumin, immunoglobulin A, and immunoglobulin G with selected strains of group A and G streptococci. Infect Immun. 1979 Jul;25(1):1–10. doi: 10.1128/iai.25.1.1-10.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miörner H., Johansson G., Kronvall G. Lipoteichoic acid is the major cell wall component responsible for surface hydrophobicity of group A streptococci. Infect Immun. 1983 Jan;39(1):336–343. doi: 10.1128/iai.39.1.336-343.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Myhre E. B., Kronvall G. Demonstration of specific binding sites for human serum albumin in group C and G streptococci. Infect Immun. 1980 Jan;27(1):6–14. doi: 10.1128/iai.27.1.6-14.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ofek I., Beachey E. H., Jefferson W., Campbell G. L. Cell membrane-binding properties of group A streptococcal lipoteichoic acid. J Exp Med. 1975 May 1;141(5):990–1003. doi: 10.1084/jem.141.5.990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ofek I., Simpson W. A., Beachey E. H. Formation of molecular complexes between a structurally defined M protein and acylated or deacylated lipoteichoic acid of Streptococcus pyogenes. J Bacteriol. 1982 Feb;149(2):426–433. doi: 10.1128/jb.149.2.426-433.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosenberg M., Perry A., Bayer E. A., Gutnick D. L., Rosenberg E., Ofek I. Adherence of Acinetobacter calcoaceticus RAG-1 to human epithelial cells and to hexadecane. Infect Immun. 1981 Jul;33(1):29–33. doi: 10.1128/iai.33.1.29-33.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Simpson W. A., Ofek I., Beachey E. H. Binding of streptococcal lipoteichoic acid to the fatty acid binding sites on serum albumin. J Biol Chem. 1980 Jul 10;255(13):6092–6097. [PubMed] [Google Scholar]
  14. Van Oss C. J., Gillman C. F. Phagocytosis as a surface phenomenon. II. Contact angles and phagocytosis of encapsulated bacteria before and after opsonization by specific antiserum and complement. J Reticuloendothel Soc. 1972 Nov;12(5):497–502. [PubMed] [Google Scholar]
  15. Whitnack E., Bisno A. L., Beachey E. H. Hyaluronate capsule prevents attachment of group A streptococci to mouse peritoneal macrophages. Infect Immun. 1981 Mar;31(3):985–991. doi: 10.1128/iai.31.3.985-991.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Widebäck K., Seal U. S., Kronvall G. Receptor in group C and G streptococci detects albumin structures present in mammalian species. Infect Immun. 1982 May;36(2):469–475. doi: 10.1128/iai.36.2.469-475.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. van Oss C. J. Phagocytosis as a surface phenomenon. Annu Rev Microbiol. 1978;32:19–39. doi: 10.1146/annurev.mi.32.100178.000315. [DOI] [PubMed] [Google Scholar]

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