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. 1983 Apr;154(1):261–268. doi: 10.1128/jb.154.1.261-268.1983

Effect of decoyinine on peptidoglycan synthesis and turnover in Bacillus subtilis.

B Uratani, J M Lopez, E Freese
PMCID: PMC217455  PMID: 6403504

Abstract

The sporulation of Bacillus subtilis can be induced in the presence of amino acids and glucose by partially depriving the cells of guanine nucleotides. This can be achieved, e.g., by the addition of decoyinine, a specific inhibitor of GMP synthetase. To determine the effect of this and other inhibitors on cell wall synthesis, we measured in their presence the incorporation of acetylglucosamine into acid-precipitable material. The rate of wall synthesis decreased by 50% within 5 min after decoyinine addition; this decrease was prevented by the presence of guanosine. A comparison with the effects of other inhibitors of cell wall synthesis indicated that decoyinine inhibited the final portion of the cell wall biosynthetic pathway, i.e., after the steps inhibited by bacitracin or vancomycin. Decoyinine addition also prevented cellular autolysis and cell wall turnover. It is not known whether these two effects of decoyinine on cell wall synthesis are causally related.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown W. C., Young F. E. Dynamic interactions between cell wall polymers, extracellular proteases and autolytic enzymes. Biochem Biophys Res Commun. 1970 Feb 20;38(4):564–568. doi: 10.1016/0006-291x(70)90618-2. [DOI] [PubMed] [Google Scholar]
  2. Fan D. P., Beckman M. M. Mutant of Bacillus subtilis demonstrating the requirement of lysis for growth. J Bacteriol. 1971 Feb;105(2):629–636. doi: 10.1128/jb.105.2.629-636.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Freese E. B., Cole R. M., Klofat W., Freese E. Growth, sporulation, and enzyme defects of glucosamine mutants of Bacillus subtilis. J Bacteriol. 1970 Mar;101(3):1046–1062. doi: 10.1128/jb.101.3.1046-1062.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Freese E., Heinze J. E., Galliers E. M. Partial purine deprivation causes sporulation of Bacillus subtilis in the presence of excess ammonia, glucose and phosphate. J Gen Microbiol. 1979 Nov;115(1):193–205. doi: 10.1099/00221287-115-1-193. [DOI] [PubMed] [Google Scholar]
  5. Frehel C., Beaufils A. M., Ryter A. Etude au microscope électronique de la croissance de la paroi chez B. subtilis et B. megaterium. Ann Inst Pasteur (Paris) 1971 Aug;121(2):139–148. [PubMed] [Google Scholar]
  6. Glaser L., Lindsay B. Relation between cell wall turnover and cell growth in Bacillus subtilis. J Bacteriol. 1977 May;130(2):610–619. doi: 10.1128/jb.130.2.610-619.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldberg I., Walker J. R., Bloch K. Inhibition of lipid synthesis in Escherichia coli cells by the antibiotic cerulenin. Antimicrob Agents Chemother. 1973 May;3(5):549–554. doi: 10.1128/aac.3.5.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ishiguro E. E., Ramey W. D. Stringent control of peptidoglycan biosynthesis in Escherichia coli K-12. J Bacteriol. 1976 Sep;127(3):1119–1126. doi: 10.1128/jb.127.3.1119-1126.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lopez J. M., Marks C. L., Freese E. The decrease of guanine nucleotides initiates sporulation of Bacillus subtilis. Biochim Biophys Acta. 1979 Oct 4;587(2):238–252. doi: 10.1016/0304-4165(79)90357-x. [DOI] [PubMed] [Google Scholar]
  10. O Y. K., Freese E. B., Freese E. Abnormal septation and inhibition of sporulation by accumulation of L- -glycerophosphate in Bacillus subtilis mutants. J Bacteriol. 1973 Feb;113(2):1034–1045. doi: 10.1128/jb.113.2.1034-1045.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ochi K., Kandala J. C., Freese E. Initiation of Bacillus subtilis sporulation by the stringent response to partial amino acid deprivation. J Biol Chem. 1981 Jul 10;256(13):6866–6875. [PubMed] [Google Scholar]
  12. Omura S. The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis. Bacteriol Rev. 1976 Sep;40(3):681–697. doi: 10.1128/br.40.3.681-697.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pitel D. W., Gilvarg C. Mucopeptide metabolism during growth and sporulation in Bacillus megaterium. J Biol Chem. 1970 Dec 25;245(24):6711–6717. [PubMed] [Google Scholar]
  14. Pooley H. M. Turnover and spreading of old wall during surface growth of Bacillus subtilis. J Bacteriol. 1976 Mar;125(3):1127–1138. doi: 10.1128/jb.125.3.1127-1138.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ramey W. D., Ishiguro E. E. Site of inhibition of peptidoglycan biosynthesis during the stringent response in Escherichia coli. J Bacteriol. 1978 Jul;135(1):71–77. doi: 10.1128/jb.135.1.71-77.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rogers H. J. Biogenesis of the wall in bacterial morphogenesis. Adv Microb Physiol. 1979;19:1–62. doi: 10.1016/s0065-2911(08)60197-6. [DOI] [PubMed] [Google Scholar]
  17. Shungu D. L., Cornett J. B., Shockman G. D. Morphological and physiological study of autolytic-defective Streptococcus faecium strains. J Bacteriol. 1979 May;138(2):598–608. doi: 10.1128/jb.138.2.598-608.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Uratani-Wong B., Lopez J. M., Freese E. Induction of citric acid cycle enzymes during initiation of sporulation by guanine nucleotide deprivation. J Bacteriol. 1981 Apr;146(1):337–344. doi: 10.1128/jb.146.1.337-344.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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