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. 1983 Apr;154(1):294–303. doi: 10.1128/jb.154.1.294-303.1983

Products of phospholipid metabolism in Bacillus stearothermophilus.

G L Card, D J Finn
PMCID: PMC217459  PMID: 6833180

Abstract

The products of phospholipid turnover in Bacillus stearothermophilus were determined in cultures labeled to equilibrium and with short pulses of [32P]phosphate and [2-3H]glycerol. Label lost from the cellular lipid pool was recovered in three fractions: low-molecular-weight extracellular products, extracellular lipid, and lipoteichoic acid (LTA). The low-molecular-weight turnover products were released from the cells during the first 10 to 20 min of a 60-min chase period and appeared to be derived primarily from phosphatidylglycerol turnover. Phosphatidylethanolamine, which appeared to be synthesized in part from the phosphatidyl group of phosphatidylglycerol, was released from the cell but was not degraded. The major product of phospholipid turnover was LTA. Essentially all of the label lost from the lipid pool during the final 40 min of the chase period was recovered as extracellular LTA. The LTA appeared to be derived primarily from the turnover of cardiolipin and the phosphatidyl group of phosphatidylglycerol. Three types of LTA were isolated; an extracellular LTA was recovered from the culture medium, and two types of LTA were extracted from membrane preparations or whole-cell lysates by the hot phenol-water procedure. Cells contained 1.5 to 2.5 mg of cellular LTA per g of cells (dry weight), over 50% of which remained associated with the membrane when cells were fractionated. Over 75% of the 3H label incorporated into the cellular LTA pool during a 90-min labeling period was released from the cells during the first cell doubling after the chase. Label lost from the lipid pool was incorporated into cellular LTA which was then modified and released into the culture medium.

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Selected References

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  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Ballesta J. P., De Garcia C. L., Schaechter M. Turnover of phosphatidylglycerol in Escherichia coli. J Bacteriol. 1973 Oct;116(1):210–214. doi: 10.1128/jb.116.1.210-214.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brissette J. L., Shockman G. D., Pieringer R. A. Effects of penicillin on synthesis and excretion of lipid and lipoteichoic acid from Streptococcus mutans BHT. J Bacteriol. 1982 Aug;151(2):838–844. doi: 10.1128/jb.151.2.838-844.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Card G. L., Georgi C. E., Militzer W. E. Phospholipids from Bacillus stearothermophilus. J Bacteriol. 1969 Jan;97(1):186–192. doi: 10.1128/jb.97.1.186-192.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Card G. L. Metabolism of phosphatidylglycerol, phosphatidylethanolamine, and cardiolipin of Bacillus stearothermophilus. J Bacteriol. 1973 Jun;114(3):1125–1137. doi: 10.1128/jb.114.3.1125-1137.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Card G. L., Szuba J. C., Shimizu M. Protein-associated lipid of Bacillus stearothermophilus. J Bacteriol. 1979 Oct;140(1):220–228. doi: 10.1128/jb.140.1.220-228.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carson D. D., Pieringer R. A., Daneo-Moore L. Effect of cerulenin on cellular autolytic activity and lipid metabolism during inhibition of protein synthesis in Streptococcus faecalis. J Bacteriol. 1981 May;146(2):590–604. doi: 10.1128/jb.146.2.590-604.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cleveland R. F., Daneo-Moore L., Wicken A. J., Shockman G. D. Effect of lipoteichoic acid and lipids on lysis of intact cells of Streptococcus faecalis. J Bacteriol. 1976 Sep;127(3):1582–1584. doi: 10.1128/jb.127.3.1582-1584.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cronan J. E., Jr Molecular biology of bacterial membrane lipids. Annu Rev Biochem. 1978;47:163–189. doi: 10.1146/annurev.bi.47.070178.001115. [DOI] [PubMed] [Google Scholar]
  10. Eibl H., Kovatchev S. Preparation of phospholipids and their analogs by phospholipase D. Methods Enzymol. 1981;72:632–639. doi: 10.1016/s0076-6879(81)72055-x. [DOI] [PubMed] [Google Scholar]
  11. Emdur L. I., Chiu T. H. Turnover of phosphatidylglycerol in Streptococcus sanguis. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1137–1144. doi: 10.1016/s0006-291x(74)80097-5. [DOI] [PubMed] [Google Scholar]
  12. Emdur L., Chiu T. The role of phosphatidylglycerol in the in vitro biosynthesis of teichoic acid and lipoteichoic acid. FEBS Lett. 1975 Jul 15;55(1):216–219. doi: 10.1016/0014-5793(75)80995-1. [DOI] [PubMed] [Google Scholar]
  13. Finnerty W. R. Physiology and biochemistry of bacterial phospholipid metabolism. Adv Microb Physiol. 1978;18:177–133. doi: 10.1016/s0065-2911(08)60417-8. [DOI] [PubMed] [Google Scholar]
  14. Fischer W., Koch H. U., Rösel P., Fiedler F., Schmuck L. Structural requirements of lipoteichoic acid carrier for recognition by the poly(ribitol phosphate) polymerase from Staphylococcus aureus H. A study of various lipoteichoic acids, derivatives, and related compounds. J Biol Chem. 1980 May 25;255(10):4550–4556. [PubMed] [Google Scholar]
  15. Ganfield M. C., Pieringer R. A. The biosynthesis of nascent membrane lipoteichoic acid of Streptococcus faecium (S. faecalis ATCC 9790) from phosphatidylkojibiosyl diacylglycerol and phosphatidylglycerol. J Biol Chem. 1980 Jun 10;255(11):5164–5169. [PubMed] [Google Scholar]
  16. Glaser L., Lindsay B. The synthesis of lipoteichoic acid carrier. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1131–1136. doi: 10.1016/s0006-291x(74)80096-3. [DOI] [PubMed] [Google Scholar]
  17. Huff E., Cole R. M., Theodore T. S. Lipoteichoic acid localization in mesosomal vesicles of Staphylococcus aureus. J Bacteriol. 1974 Oct;120(1):273–281. doi: 10.1128/jb.120.1.273-281.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Huff E. Lipoteichoic acid, a major amphiphile of Gram-positive bacteria that is not readily extractable. J Bacteriol. 1982 Jan;149(1):399–402. doi: 10.1128/jb.149.1.399-402.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Imanaka T., Fujii M., Aramori I., Aiba S. Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis. J Bacteriol. 1982 Mar;149(3):824–830. doi: 10.1128/jb.149.3.824-830.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Joseph R., Shockman G. D. Cellular localization of lipoteichoic acid in Streptococcus faecalis. J Bacteriol. 1975 Jun;122(3):1375–1386. doi: 10.1128/jb.122.3.1375-1386.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kennedy L. D. Teichoic acid synthesis in Bacillus stearothermophilus. Biochem J. 1974 Mar;138(3):525–535. doi: 10.1042/bj1380525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kessler R. E., Shockman G. D. Precursor-product relationship of intracellular and extracellular lipoteichoic acids of Streptococcus faecium. J Bacteriol. 1979 Feb;137(2):869–877. doi: 10.1128/jb.137.2.869-877.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lambert P. A., Hancock I. C., Baddiley J. Occurrence and function of membrane teichoic acids. Biochim Biophys Acta. 1977 May 31;472(1):1–12. doi: 10.1016/0304-4157(77)90012-0. [DOI] [PubMed] [Google Scholar]
  24. Lombardi F. J., Chen S. L., Fulco A. J. A rapidly metabolizing pool of phosphatidylglycerol as a precursor of phosphatidylethanolamine and diglyceride in Bacillus megaterium. J Bacteriol. 1980 Feb;141(2):626–634. doi: 10.1128/jb.141.2.626-634.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Lombardi F. J., Fulco A. J. Two distinct pools of membrane phosphatidylglycerol in Bacillus megaterium. J Bacteriol. 1980 Feb;141(2):618–625. doi: 10.1128/jb.141.2.618-625.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. ROE J. H. The determination of sugar in blood and spinal fluid with anthrone reagent. J Biol Chem. 1955 Jan;212(1):335–343. [PubMed] [Google Scholar]
  27. Raetz C. R. Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli. Microbiol Rev. 1978 Sep;42(3):614–659. doi: 10.1128/mr.42.3.614-659.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Rowe J. J., Goldberg I. D., Amelunxen R. E. Development of defined and minimal media for the growth of Bacillus stearothermophilus. J Bacteriol. 1975 Oct;124(1):279–284. doi: 10.1128/jb.124.1.279-284.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Schulman H., Kennedy E. P. Relation of turnover of membrane phospholipids to synthesis of membrane-derived oligosaccharides of Escherichia coli. J Biol Chem. 1977 Jun 25;252(12):4250–4255. [PubMed] [Google Scholar]
  30. Short S. A., White D. C. Metabolism of phosphatidylglycerol, lysylphosphatidylglycerol, and cardiolipin of Staphylococcus aureus. J Bacteriol. 1971 Oct;108(1):219–226. doi: 10.1128/jb.108.1.219-226.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tucker A. N., White D. C. Detection of a rapidly metabolizing portion of the membrane cardiolipin in Haemophilus parainfluenzae. J Bacteriol. 1971 Dec;108(3):1058–1064. doi: 10.1128/jb.108.3.1058-1064.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Yokota K., Kito M. Transfer of the phosphatidyl moiety of phosphatidylglycerol to phosphatidylethanolamine in Escherichia coli. J Bacteriol. 1982 Aug;151(2):952–961. doi: 10.1128/jb.151.2.952-961.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. de Boer W. R., Wouters J. T., Anderson A. J., Archibald A. R. Further evidence for the structure of the teichoic acids from Bacillus stearothermophilus B65 and Bacillus subtilis var. niger WM. Eur J Biochem. 1978 Apr 17;85(2):433–436. doi: 10.1111/j.1432-1033.1978.tb12256.x. [DOI] [PubMed] [Google Scholar]

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