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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Apr;154(1):383–386. doi: 10.1128/jb.154.1.383-386.1983

Locus of the Pseudomonas aeruginosa toxin A gene.

L F Hanne, T R Howe, B H Iglewski
PMCID: PMC217470  PMID: 6403508

Abstract

The gene for Pseudomonas aeruginosa toxin A has been mapped in the late region of the chromosome of strain PAO. Strain PAO-PR1, which produces parental levels of toxin A antigen that is enzymatically inactive and nontoxic, was used as the donor for R68.45 plasmid-mediated genetic exchange. Strain PAO-PR1 (toxA1) was mated with toxin A-producing strains, and exconjugates for selected prototrophic markers were tested for the transfer of toxA1. The toxA1 gene was located between cnu-9001 and pur-67 at approximately 85 min on the PAO chromosome.

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Selected References

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