Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 May;154(2):992–994. doi: 10.1128/jb.154.2.992-994.1983

Effects of aerobic and anaerobic shock on catabolite repression in cyclic AMP suppressor mutants of Escherichia coli.

J H Lee, W J Dobrogosz
PMCID: PMC217556  PMID: 6302089

Abstract

Cultures of Escherichia coli K-12 grown on glucose or gluconate under aerobic conditions exhibited catabolite repression of beta-galactosidase synthesis. Depression occurred when these cultures were subjected to anaerobic shock. These states of repression and depression were found to be associated with low and high differential rates of cyclic AMP synthesis, respectively. This observation is consistent with the view that cyclic AMP plays a central role in the catabolite repression phenomenon. We report here, however, that identical stages of repression and derepression occur in mutant strains possessing cya crp(Csm) genotypes and therefore unable to synthesize cyclic AMP. These results suggest that cyclic AMP is not the sole regulator involved in catabolite repression.

Full text

PDF
992

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander J. K. Suppression of defects in cyclic adenosine 3',5'-monophosphate metabolism in Escherichia coli. J Bacteriol. 1980 Oct;144(1):205–209. doi: 10.1128/jb.144.1.205-209.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Botsford J. L. Cyclic nucleotides in procaryotes. Microbiol Rev. 1981 Dec;45(4):620–642. doi: 10.1128/mr.45.4.620-642.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Botsford J. L., Drexler M. The cyclic 3',5'-adenosine monophosphate receptor protein and regulation of cyclic 3',5'-adenosine monophosphate synthesis in Escherichia coli. Mol Gen Genet. 1978 Sep 20;165(1):47–56. doi: 10.1007/BF00270375. [DOI] [PubMed] [Google Scholar]
  4. Botsford J. L. Metabolism of cyclic adenosine 3',5'-monophosphate and induction of tryptophanase in Escherichia coli. J Bacteriol. 1975 Oct;124(1):380–390. doi: 10.1128/jb.124.1.380-390.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buettner M. J., Spitz E., Rickenberg H. V. Cyclic adenosine 3',5'-monophosphate in Escherichia coli. J Bacteriol. 1973 Jun;114(3):1068–1073. doi: 10.1128/jb.114.3.1068-1073.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. COHN M., HORIBATA K. Physiology of the inhibition by glucose of the induced synthesis of the beta-galactosideenzyme system of Escherichia coli. J Bacteriol. 1959 Nov;78:624–635. doi: 10.1128/jb.78.5.624-635.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cheung W. Y. Cyclic 3'.5'-nucleotide phosphodiesterase. Effect of binding protein on the hydrolysis of cyclic AMP. Biochem Biophys Res Commun. 1972 Jan 14;46(1):99–105. doi: 10.1016/0006-291x(72)90635-3. [DOI] [PubMed] [Google Scholar]
  8. DOBROGOSZ W. J. THE INFLUENCE OF NITRATE AND NITRITE REDUCTION ON CATABOLITE REPRESSION IN ESCHERICHIA COLI. Biochim Biophys Acta. 1965 May 4;100:553–566. doi: 10.1016/0304-4165(65)90025-5. [DOI] [PubMed] [Google Scholar]
  9. Dessein A., Schwartz M., Ullmann A. Catabolite repression in Escherichia coli mutants lacking cyclic AMP. Mol Gen Genet. 1978 Jun 1;162(1):83–87. doi: 10.1007/BF00333853. [DOI] [PubMed] [Google Scholar]
  10. Epstein W., Rothman-Denes L. B., Hesse J. Adenosine 3':5'-cyclic monophosphate as mediator of catabolite repression in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2300–2304. doi: 10.1073/pnas.72.6.2300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fraser D. E., Yamazaki H. Construction of an Escherichia coli strain which excretes abnormally large amounts of adenosine 3',5'-cyclic monophosphate. Can J Microbiol. 1978 Nov;24(11):1423–1425. doi: 10.1139/m78-228. [DOI] [PubMed] [Google Scholar]
  12. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Goldenbaum P. E., Broman R. L., Dobrogosz W. J. Cyclic 3',5'-adenosine monophosphate and N-acetylglucosamine-6-phosphate as regulatory signals in catabolite repression of the lac operon in Escherichia coli. J Bacteriol. 1970 Sep;103(3):663–670. doi: 10.1128/jb.103.3.663-670.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Haggerty D. M., Schleif R. F. Kinetics of the onset of catabolite repression in Escherichia coli as determined by lac messenger ribonucleic acid initiations and intracellular cyclic adenosine 3',5'-monophosphate levels. J Bacteriol. 1975 Sep;123(3):946–953. doi: 10.1128/jb.123.3.946-953.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Melton T., Snow L. L., Freitag C. S., Dobrogosz W. J. Isolation and characterization of cAMP suppressor mutants of Escherichia coli K12. Mol Gen Genet. 1981;182(3):480–489. doi: 10.1007/BF00293939. [DOI] [PubMed] [Google Scholar]
  16. Okinaka R. T., Dobrogosz W. J. Catabolite repression and pyruvate metabolism in Escherichia coli. J Bacteriol. 1967 May;93(5):1644–1650. doi: 10.1128/jb.93.5.1644-1650.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pastan I., Adhya S. Cyclic adenosine 5'-monophosphate in Escherichia coli. Bacteriol Rev. 1976 Sep;40(3):527–551. doi: 10.1128/br.40.3.527-551.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takebe Y., Shibuya M., Kaziro Y. A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP. J Biochem. 1978 Jun;83(6):1615–1623. doi: 10.1093/oxfordjournals.jbchem.a132073. [DOI] [PubMed] [Google Scholar]
  19. Wayne P. K., Rosen O. M. Cyclic 3':5'-adenosine monophosphate in Escherichia coli during transient and catabolite repression. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1436–1440. doi: 10.1073/pnas.71.4.1436. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES