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. 1983 May;154(2):1009–1012. doi: 10.1128/jb.154.2.1009-1012.1983

traJ independence in expression of traT on F.

A Rashtchian, J H Crooks, S B Levy
PMCID: PMC217561  PMID: 6341352

Abstract

Studies of F plasmids in the same cell as a transfer-repressed IncFII R plasmid showed a 100 to 1,000-fold decrease in transfer and a 75-fold decrease in surface exclusion, but no detectable change was shown in the amount of TraTp synthesized. Moreover, a mutation in traJ on F which caused a 10(4)-fold reduction in transfer caused only a 3.6-fold decrease in TraTp. These two findings suggest that a significant amount of traT expression on F is independent of traJ. Furthermore, we showed, using immunoprecipitation of TraTp, that normal amounts of this protein could be present in the cell without producing normal levels of surface exclusion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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