Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 May;154(2):1015–1020. doi: 10.1128/jb.154.2.1015-1020.1983

Immunochemical relationship of the major outer membrane protein of Rhodopseudomonas sphaeroides 2.4.1 to proteins of other photosynthetic bacteria.

C D Deal, S Kaplan
PMCID: PMC217563  PMID: 6188744

Abstract

Immunoblots of sodium dodecyl sulfate-polyacrylamide gels derived from outer membrane preparations of various strains of Rhodopseudomonas sphaeroides revealed polypeptides which cross-reacted with antibody directed against the major outer membrane protein of R. sphaeroides 2.4.1. Immunochemical quantitation of the major outer membrane protein of strain 2.4.1 showed approximately 5.5 x 10(4) molecules per cell whether cells were grown chemoheterotrophically or photoheterotrophically. Rhodospirillum rubrum outer membranes contained a cross-reactive protein, whereas the outer membranes derived from Rhodopseudomonas capsulata and Paracoccus denitrificans showed no cross-reaction with the antibody prepared against the major outer membrane protein from R. sphaeroides 2.4.1.

Full text

PDF
1015

Images in this article

1016Image
on p.1016
1017Image
on p.1017
1017Image
on p.1017

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baumgardner D., Deal C., Kaplan S. Protein composition of Rhodopseudomonas sphaeroides outer membrane. J Bacteriol. 1980 Jul;143(1):265–273. doi: 10.1128/jb.143.1.265-273.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  3. Ding D. H., Kaplan S. Separation of inner and outer membranes of Rhodopseudomonas spheroides. Prep Biochem. 1976;6(1):61–79. doi: 10.1080/00327487608061599. [DOI] [PubMed] [Google Scholar]
  4. Donohue T. J., Cain B. D., Kaplan S. Alterations in the phospholipid composition of Rhodopseudomonas sphaeroides and other bacteria induced by Tris. J Bacteriol. 1982 Nov;152(2):595–606. doi: 10.1128/jb.152.2.595-606.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fornari C. S., Kaplan S. Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA. J Bacteriol. 1982 Oct;152(1):89–97. doi: 10.1128/jb.152.1.89-97.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
  7. Hofstra H., Dankert J. Porin from the outer membrane of Escherichia coli: immunological characterization of native and heat-dissociated forms. J Gen Microbiol. 1981 Aug;125(2):285–292. doi: 10.1099/00221287-125-2-285. [DOI] [PubMed] [Google Scholar]
  8. Howe J. G., Hershey J. W. A sensitive immunoblotting method for measuring protein synthesis initiation factor levels in lysates of Escherichia coli. J Biol Chem. 1981 Dec 25;256(24):12836–12839. [PubMed] [Google Scholar]
  9. Kranz R. G., Gennis R. B. Isoelectric focusing and crossed immunoelectrophoresis of heme proteins in the Escherichia coli cytoplasmic membrane. J Bacteriol. 1982 Apr;150(1):36–45. doi: 10.1128/jb.150.1.36-45.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. SISTROM W. R. A requirement for sodium in the growth of Rhodopseudomonas spheroides. J Gen Microbiol. 1960 Jun;22:778–785. doi: 10.1099/00221287-22-3-778. [DOI] [PubMed] [Google Scholar]
  12. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  13. Shepherd W. D., Kaplan S., Park J. T. Penicillin-binding proteins of Rhodopseudomonas sphaeroides and their membrane localization. J Bacteriol. 1981 Aug;147(2):354–361. doi: 10.1128/jb.147.2.354-361.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smyth C. J., Siegel J., Salton M. R., Owen P. Immunochemical analysis of inner and outer membranes of Escherichia coli by crossed immunoelectrophoresis. J Bacteriol. 1978 Jan;133(1):306–319. doi: 10.1128/jb.133.1.306-319.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES