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. 1993 Jun;109(2):577–580. doi: 10.1111/j.1476-5381.1993.tb13609.x

Kinetic study of the tubular dopamine outward transporter in the rat and dog kidney.

P Soares-da-Silva 1
PMCID: PMC2175669  PMID: 8358558

Abstract

1. The present study has determined the kinetic characteristics of the outflow of dopamine of renal origin in slices of rat and dog renal cortex loaded with exogenous L-dihydroxyphenylalanine (L-DOPA 5 to 5000 microM). 2. In both dog and rat renal tissues the production of dopamine was found to be dependent on the concentration of L-DOPA used and reached its maximum at 2500 microM L-DOPA. The decarboxylation of L-DOPA in rat cortical slices (16.4 +/- 2.6 to 1479.2 +/- 85.2 nmol g-1) was 6 fold that in the dog (2.2 +/- 0.4 to 252.1 +/- 21.2 nmol g-1). In the rat kidney a large amount (approximately 50%) of the dopamine (5.2 +/- 0.6 to 743.4 +/- 58.3 nmol g-1) was found to escape into the incubation medium, whereas in dog renal slices the amount of newly-formed dopamine escaping into the incubation medium (0.7 +/- 0.2 to 46.5 +/- 9.3 nmol g-1) was less than 25% of the total amount of the amine formed. 3. The application of the Michaelis-Menten equation to the net transport of newly-formed dopamine has allowed the identification of a saturable (carrier-mediated transfer) and a non-saturable component (diffusion). The Vmax (nmol g-1 15 min-1) and Km (nM) values for the saturable component were, respectively, 340 +/- 41 and 396 +/- 45 in the rat kidney and 112 +/- 16 and 319 +/- 35 in the dog kidney.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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