Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1993 Jun;109(2):415–423. doi: 10.1111/j.1476-5381.1993.tb13585.x

S-nitrosocysteine, but not sodium nitroprusside, produces apamin-sensitive hyperpolarization in rat gastric fundus.

K Kitamura 1, Q Lian 1, A Carl 1, H Kuriyama 1
PMCID: PMC2175683  PMID: 8395287

Abstract

1. To investigate the pharmacological properties of the membrane hyperpolarization induced by electrical field stimulation (EFS), sodium nitroprusside (SNP) and S-nitrosocysteine (NO-Cys) in circular smooth muscle cells of the rat gastric fundus (forestomach), the effects of various potassium channel blockers on these hyperpolarizations were investigated. 2. EFS (50 microseconds, 20 Hz, 3 pulses, 10-50 V) produced inhibitory junction potentials (i.j.ps), in the presence of atropine (1 microM) and guanethidine (1 microM). NO-Cys and SNP produced hyperpolarization of the membrane in the rat gastric fundus. L-NG-nitroarginine (L-NNA) inhibited the i.j.ps, but not the hyperpolarization induced by NO-Cys and SNP. This inhibitory action of L-NNA on the i.j.ps was partly reversed by subsequent application of L-arginine (1 mM) but not by D-arginine. 3. Oxyhaemoglobin (Oxy-Hb; 5 microM) inhibited these hyperpolarizations, although a higher concentration of Oxy-Hb was required to inhibit the SNP-induced hyperpolarization. Hydroquinone (50 microM) inhibited only the hyperpolarization induced by NO-Cys. 4. Apamin (1 microM) partly inhibited i.j.ps and NO-Cys-induced hyperpolarization, but not the SNP-induced hyperpolarization. Tetraethylammonium (TEA; 1 mM), 4-aminopyridine (4-AP; 1 mM) or glibenclamide (1 microM) did not affect hyperpolarization induced by NO-Cys and SNP. 5. 8-Bromo cyclic guanosine 3':5'-monophosphate (1 mM) also produced hyperpolarization. Apamin (1 microM), TEA (1 mM) and glibenclamide (5 microM) all failed to inhibit this hyperpolarization.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
415

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banks B. E., Brown C., Burgess G. M., Burnstock G., Claret M., Cocks T. M., Jenkinson D. H. Apamin blocks certain neurotransmitter-induced increases in potassium permeability. Nature. 1979 Nov 22;282(5737):415–417. doi: 10.1038/282415a0. [DOI] [PubMed] [Google Scholar]
  2. Bauer V., Kuriyama H. Evidence for non-cholinergic, non-adrenergic transmission in the guinea-pig ileum. J Physiol. 1982 Sep;330:95–110. doi: 10.1113/jphysiol.1982.sp014331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blatz A. L., Magleby K. L. Single apamin-blocked Ca-activated K+ channels of small conductance in cultured rat skeletal muscle. Nature. 1986 Oct 23;323(6090):718–720. doi: 10.1038/323718a0. [DOI] [PubMed] [Google Scholar]
  4. Boeckxstaens G. E., Pelckmans P. A., Bogers J. J., Bult H., De Man J. G., Oosterbosch L., Herman A. G., Van Maercke Y. M. Release of nitric oxide upon stimulation of nonadrenergic noncholinergic nerves in the rat gastric fundus. J Pharmacol Exp Ther. 1991 Feb;256(2):441–447. [PubMed] [Google Scholar]
  5. Bult H., Boeckxstaens G. E., Pelckmans P. A., Jordaens F. H., Van Maercke Y. M., Herman A. G. Nitric oxide as an inhibitory non-adrenergic non-cholinergic neurotransmitter. Nature. 1990 May 24;345(6273):346–347. doi: 10.1038/345346a0. [DOI] [PubMed] [Google Scholar]
  6. Cheung D. W., MacKay M. J. The effects of sodium nitroprusside and 8-bromo-cyclic GMP on electrical and mechanical activities of the rat tail artery. Br J Pharmacol. 1985 Sep;86(1):117–124. doi: 10.1111/j.1476-5381.1985.tb09441.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fujino K., Nakaya S., Wakatsuki T., Miyoshi Y., Nakaya Y., Mori H., Inoue I. Effects of nitroglycerin on ATP-induced Ca(++)-mobilization, Ca(++)-activated K channels and contraction of cultured smooth muscle cells of porcine coronary artery. J Pharmacol Exp Ther. 1991 Jan;256(1):371–377. [PubMed] [Google Scholar]
  8. Garland C. J., McPherson G. A. Evidence that nitric oxide does not mediate the hyperpolarization and relaxation to acetylcholine in the rat small mesenteric artery. Br J Pharmacol. 1992 Feb;105(2):429–435. doi: 10.1111/j.1476-5381.1992.tb14270.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gillespie J. S., Sheng H. The effects of pyrogallol and hydroquinone on the response to NANC nerve stimulation in the rat anococcygeus and the bovine retractor penis muscles. Br J Pharmacol. 1990 Jan;99(1):194–196. doi: 10.1111/j.1476-5381.1990.tb14677.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gruetter C. A., Kadowitz P. J., Ignarro L. J. Methylene blue inhibits coronary arterial relaxation and guanylate cyclase activation by nitroglycerin, sodium nitrite, and amyl nitrite. Can J Physiol Pharmacol. 1981 Feb;59(2):150–156. doi: 10.1139/y81-025. [DOI] [PubMed] [Google Scholar]
  11. Horio Y., Murad F. Purification of guanylyl cyclase from rod outer segments. Biochim Biophys Acta. 1991 Dec 3;1133(1):81–88. doi: 10.1016/0167-4889(91)90244-r. [DOI] [PubMed] [Google Scholar]
  12. Ignarro L. J., Buga G. M., Wood K. S., Byrns R. E., Chaudhuri G. Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9265–9269. doi: 10.1073/pnas.84.24.9265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Inoue T., Kanmura Y., Fujisawa K., Itoh T., Kuriyama H. Effects of 2-nicotinamidoethyl nitrate (nicorandil; SG-75) and its derivative on smooth muscle cells of the canine mesenteric artery. J Pharmacol Exp Ther. 1984 Jun;229(3):793–802. [PubMed] [Google Scholar]
  14. Kajioka S., Oike M., Kitamura K. Nicorandil opens a calcium-dependent potassium channel in smooth muscle cells of the rat portal vein. J Pharmacol Exp Ther. 1990 Sep;254(3):905–913. [PubMed] [Google Scholar]
  15. Komori K., Suzuki H. Distribution and properties of excitatory and inhibitory junction potentials in circular muscle of the guinea-pig stomach. J Physiol. 1986 Jan;370:339–355. doi: 10.1113/jphysiol.1986.sp015938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kowaluk E. A., Seth P., Fung H. L. Metabolic activation of sodium nitroprusside to nitric oxide in vascular smooth muscle. J Pharmacol Exp Ther. 1992 Sep;262(3):916–922. [PubMed] [Google Scholar]
  17. Li C. G., Rand M. J. Evidence for a role of nitric oxide in the neurotransmitter system mediating relaxation of the rat anococcygeus muscle. Clin Exp Pharmacol Physiol. 1989 Dec;16(12):933–938. doi: 10.1111/j.1440-1681.1989.tb02404.x. [DOI] [PubMed] [Google Scholar]
  18. Martin W., Villani G. M., Jothianandan D., Furchgott R. F. Selective blockade of endothelium-dependent and glyceryl trinitrate-induced relaxation by hemoglobin and by methylene blue in the rabbit aorta. J Pharmacol Exp Ther. 1985 Mar;232(3):708–716. [PubMed] [Google Scholar]
  19. Mittal C. K., Murad F. Activation of guanylate cyclase by superoxide dismutase and hydroxyl radical: a physiological regulator of guanosine 3',5'-monophosphate formation. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4360–4364. doi: 10.1073/pnas.74.10.4360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Moncada S., Palmer R. M., Higgs E. A. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev. 1991 Jun;43(2):109–142. [PubMed] [Google Scholar]
  21. Nakao K., Inoue R., Yamanaka K., Kitamura K. Actions of quinidine and apamin on after-hyperpolarization of the spike in circular smooth muscle cells of the guinea-pig ileum. Naunyn Schmiedebergs Arch Pharmacol. 1986 Dec;334(4):508–513. doi: 10.1007/BF00569394. [DOI] [PubMed] [Google Scholar]
  22. Osthaus L. E., Galligan J. J. Antagonists of nitric oxide synthesis inhibit nerve-mediated relaxations of longitudinal muscle in guinea pig ileum. J Pharmacol Exp Ther. 1992 Jan;260(1):140–145. [PubMed] [Google Scholar]
  23. Romey G., Lazdunski M. The coexistence in rat muscle cells of two distinct classes of Ca2+-dependent K+ channels with different pharmacological properties and different physiological functions. Biochem Biophys Res Commun. 1984 Jan 30;118(2):669–674. doi: 10.1016/0006-291x(84)91355-x. [DOI] [PubMed] [Google Scholar]
  24. Standen N. B., Quayle J. M., Davies N. W., Brayden J. E., Huang Y., Nelson M. T. Hyperpolarizing vasodilators activate ATP-sensitive K+ channels in arterial smooth muscle. Science. 1989 Jul 14;245(4914):177–180. doi: 10.1126/science.2501869. [DOI] [PubMed] [Google Scholar]
  25. Stark M. E., Bauer A. J., Szurszewski J. H. Effect of nitric oxide on circular muscle of the canine small intestine. J Physiol. 1991 Dec;444:743–761. doi: 10.1113/jphysiol.1991.sp018904. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Thornbury K. D., Ward S. M., Dalziel H. H., Carl A., Westfall D. P., Sanders K. M. Nitric oxide and nitrosocysteine mimic nonadrenergic, noncholinergic hyperpolarization in canine proximal colon. Am J Physiol. 1991 Sep;261(3 Pt 1):G553–G557. doi: 10.1152/ajpgi.1991.261.3.G553. [DOI] [PubMed] [Google Scholar]
  27. Waldman S. A., Murad F. Cyclic GMP synthesis and function. Pharmacol Rev. 1987 Sep;39(3):163–196. [PubMed] [Google Scholar]
  28. Ward S. M., Dalziel H. H., Bradley M. E., Buxton I. L., Keef K., Westfall D. P., Sanders K. M. Involvement of cyclic GMP in non-adrenergic, non-cholinergic inhibitory neurotransmission in dog proximal colon. Br J Pharmacol. 1992 Dec;107(4):1075–1082. doi: 10.1111/j.1476-5381.1992.tb13409.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Ward S. M., McKeen E. S., Sanders K. M. Role of nitric oxide in non-adrenergic, non-cholinergic inhibitory junction potentials in canine ileocolonic sphincter. Br J Pharmacol. 1992 Apr;105(4):776–782. doi: 10.1111/j.1476-5381.1992.tb09056.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Williams D. L., Jr, Katz G. M., Roy-Contancin L., Reuben J. P. Guanosine 5'-monophosphate modulates gating of high-conductance Ca2+-activated K+ channels in vascular smooth muscle cells. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9360–9364. doi: 10.1073/pnas.85.23.9360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Yamanaka K., Furukawa K., Kitamura K. The different mechanisms of action of nicorandil and adenosine triphosphate on potassium channels of circular smooth muscle of the guinea-pig small intestine. Naunyn Schmiedebergs Arch Pharmacol. 1985 Oct;331(1):96–103. doi: 10.1007/BF00498857. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES