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. 1993 Dec;110(4):1491–1495. doi: 10.1111/j.1476-5381.1993.tb13990.x

PPADS selectively antagonizes P2X-purinoceptor-mediated responses in the rabbit urinary bladder.

A U Ziganshin 1, C H Hoyle 1, X Bo 1, G Lambrecht 1, E Mutschler 1, H G Bäumert 1, G Burnstock 1
PMCID: PMC2175839  PMID: 8306091

Abstract

1. Pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS), an inhibitor of P2X-purinoceptor-mediated responses in rabbit vas deferens, was investigated for its ability to antagonize contractions evoked by alpha,beta-methylene ATP (alpha,beta-MeATP), carbachol and electrical field stimulation in the rabbit urinary bladder detrusor muscle. 2. PPADS. (1-30 microM) caused concentration-dependent inhibition of contractions to the stable P2X-purinoceptor agonist, alpha,beta-MeATP, decreasing the maximum response to alpha,beta-MeATP (30 microM) at concentrations of 3-30 microM. The pD2 value for alpha,beta-MeATP in the absence of PPADS was 6.52 +/- 0.10 (8). In the presence of PPADS at concentrations of 1, 3, 10 and 30 microM the negative log concentrations of alpha,beta-MeATP that cause the same contractile response as the pD2 value were significantly different from control, being respectively 6.17 +/- 0.09 (8), 5.64 +/- 0.12 (7), 5.15 +/- 0.23 (7) and 4.78 +/- 0.22 (5). 3. PPADS (1-30 microM) caused concentration-dependent inhibition of contractions to stimulation of intramural purinergic nerves (1-32 Hz). There was a greater inhibition at lower frequencies (1-8 Hz) than at higher frequencies (16-32 Hz). PPADS, 30 microM, did not produce significantly greater antagonism than 10 microM. 4. PPADS (30 microM) had no significant influence on the contractile potency of carbachol: the pD2 values of carbachol in the absence and presence of PPADS were not significantly different being 6.42 +/- 0.16 (5) and 6.33 +/- 0.18 (5), respectively. However, PPADS caused a small, but significant, suppression of the maximal response of carbachol, reducing it by approximately 9%.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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