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. 1983 Jun;154(3):1227–1235. doi: 10.1128/jb.154.3.1227-1235.1983

In vivo cloning of Erwinia carotovora genes involved in the catabolism of hexuronates.

F Van Gijsegem, A Toussaint
PMCID: PMC217595  PMID: 6853444

Abstract

Using the RP4::mini-Mu pULB113 plasmid, an RP4 derivative carrying a deleted Mu prophage which allows the plasmid to pick up any chromosomal DNA segment to form R' plasmids, we cloned all of the genes of Erwinia carotovora involved in the catabolism of the hexuronates and in the transport of these substrates. With the R' plasmids we isolated, we performed complementation analysis and found that, in the Erwinia carotovora strain we used, the genes involved in the catabolism of the hexuronates are clustered in four regions of the chromosome. This genetic organization is compared with that of Escherichia coli K-12.

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Selected References

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