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. 1983 Jun;154(3):1269–1275. doi: 10.1128/jb.154.3.1269-1275.1983

Isolation and characterization of an Escherichia coli mutant lacking cytochrome d terminal oxidase.

G N Green, R B Gennis
PMCID: PMC217600  PMID: 6304009

Abstract

A screening procedure was devised which permitted the isolation of a cytochrome d-deficient mutant by its failure to oxidize the artificial electron donor N,N,N',N'-tetramethyl-p-phenylenediamine. Cytochrome a1 and probably cytochrome b558 were also missing in the mutant. Growth and oxygen uptake rates were similar for both parent and mutant strains. However, the strain lacking cytochrome d had an increased sensitivity to cyanide, indicating that cytochrome d confers some resistance to this respiratory inhibitor. The gene responsible for these phenotypes has been named cyd and maps between tolA and sucB.

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Selected References

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