Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1993 Sep;110(1):199–206. doi: 10.1111/j.1476-5381.1993.tb13792.x

Effects of hydroxyethylrutosides on the permeability of microvessels in the frog mesentery.

S Kendall 1, R Towart 1, C C Michel 1
PMCID: PMC2176015  PMID: 8220880

Abstract

1. We have investigated the effects of a standardised mixture of hydroxyethylrutosides (HR, Venoruton), a mixture of five of its main components (M) and each of the five components separately (7-mono-HR, 7,4'-di-HR, 7,3',4'-tri-HR, 5,7,3',4'-tetra-HR and 7,3'4'-tri HQ) upon the permeability of single perfused capillaries and venules in the mesenteries of pithed frogs. 2. In each experiment, the hydraulic permeability (Lp) of a single perfused microvessel and the effective osmotic pressure (sigma delta pi) exerted by macromolecules across its walls were estimated by a microcclusion technique, first during control perfusion and then in the presence of a known concentration of test substance. 3. HR, M and 7,4'-di-HR reduced Lp in a similar concentration-dependent manner over the range of 1 microgram ml-1 to 1 mg ml-1 (maximum reduction was to 40% of control Lp at 1 mg ml-1). At perfusate concentrations greater than 1 mg ml-1, these substances reduced Lp to a lesser extent. While the four other test substances reduced Lp significantly when their perfusate concentrations equalled or exceeded 100 micrograms ml-1, they were all less potent than 7,4'-di-HR. 4. The reduction in Lp induced by the mixture of flavonoids was only slightly reversed by subsequent perfusion with flavonoid-free solutions. 5. When permeability was increased by perfusing with protein-free solutions, both HR and 7,4'-di-HR reduced and then reversed the increase in Lp in a concentration-dependent manner over the range of 1 microgram ml-1 to 100 micrograms ml-1.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
199

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arturson G. Effects of O-( -hydroxyethyl)-rutosides (HR) on the increased microvascular permeability in experimental skin burns. Preliminary report. Acta Chir Scand. 1972;138(2):111–117. [PubMed] [Google Scholar]
  2. Balmer A., Limoni C. Klinische, plazebokontrollierte Doppelblindprüfung von Venoruton bei der Behandlung der chronisch-venösen Insuffizienz. Die Bedeutung der Patientenauswahl. Vasa. 1980;9(1):76–82. [PubMed] [Google Scholar]
  3. Blumberg S., Clough G., Michel C. Effects of hydroxyethyl rutosides upon the permeability of single capillaries in the frog mesentery. Br J Pharmacol. 1989 Apr;96(4):913–919. doi: 10.1111/j.1476-5381.1989.tb11902.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cesarone M. R., Laurora G., Ricci A., Belcaro G., Pomante P., Candiani C., Leon M., Nicolaides A. N. Acute effects of hydroxyethylrutosides on capillary filtration in normal volunteers, patients with venous hypertension and in patients with diabetic microangiopathy (a dose comparison study). Vasa. 1992;21(1):76–80. [PubMed] [Google Scholar]
  5. Curry F. E., Michel C. C. A fiber matrix model of capillary permeability. Microvasc Res. 1980 Jul;20(1):96–99. doi: 10.1016/0026-2862(80)90024-2. [DOI] [PubMed] [Google Scholar]
  6. Curry F. E., Michel C. C., Phillips M. E. Effect of albumin on the osmotic pressure exerted by myoglobin across capillary walls in frog mesentery. J Physiol. 1987 Jun;387:69–82. doi: 10.1113/jphysiol.1987.sp016563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gerdin B., Svensjö E. Inhibitory effect of the flavonoid O-(beta-hydroxyethyl)-rutoside on increased microvascular permeability induced by various agents in rat skin. Int J Microcirc Clin Exp. 1983;2(1):39–46. [PubMed] [Google Scholar]
  8. He P., Curry F. E. Depolarization modulates endothelial cell calcium influx and microvessel permeability. Am J Physiol. 1991 Oct;261(4 Pt 2):H1246–H1254. doi: 10.1152/ajpheart.1991.261.4.H1246. [DOI] [PubMed] [Google Scholar]
  9. Hilton J. G. Effects of beta-hydroxyethyl rutosides (H-R) administered post burn after thermal-injury-induced plasma volume loss in the nonresuscitated dog. Burns Incl Therm Inj. 1982 Jul;8(6):391–394. doi: 10.1016/0305-4179(82)90108-5. [DOI] [PubMed] [Google Scholar]
  10. Levick J. R., Michel C. C. A densitometric method for determining the filtration coefficients of single capillaries in the frog mesentery. Microvasc Res. 1977 Mar;13(2):141–151. doi: 10.1016/0026-2862(77)90081-4. [DOI] [PubMed] [Google Scholar]
  11. Mason J. C., Curry F. E., Michel C. C. The effects of proteins upon the filtration coefficient of individually perfused frog mesenteric capillaries. Microvasc Res. 1977 Mar;13(2):185–202. doi: 10.1016/0026-2862(77)90084-x. [DOI] [PubMed] [Google Scholar]
  12. Michel C. C. Capillary permeability and how it may change. J Physiol. 1988 Oct;404:1–29. doi: 10.1113/jphysiol.1988.sp017275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Michel C. C. Filtration coefficients and osmotic reflexion coefficients of the walls of single frog mesenteric capillaries. J Physiol. 1980 Dec;309:341–355. doi: 10.1113/jphysiol.1980.sp013512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Michel C. C., Mason J. C., Curry F. E., Tooke J. E., Hunter P. J. A development of the Landis technique for measuring the filtration coefficient of individual capillaries in the frog mesentery. Q J Exp Physiol Cogn Med Sci. 1974 Oct;59(4):283–309. doi: 10.1113/expphysiol.1974.sp002275. [DOI] [PubMed] [Google Scholar]
  15. Michel C. C., Phillips M. E. The effects of bovine serum albumin and a form of cationised ferritin upon the molecular selectivity of the walls of single frog capillaries. Microvasc Res. 1985 Mar;29(2):190–203. doi: 10.1016/0026-2862(85)90016-0. [DOI] [PubMed] [Google Scholar]
  16. Michel C. C., Phillips M. E., Turner M. R. The effects of native and modified bovine serum albumin on the permeability of frog mesenteric capillaries. J Physiol. 1985 Mar;360:333–346. doi: 10.1113/jphysiol.1985.sp015620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Neumann H. A., Carlsson K., Brom G. H. Uptake and localisation of O-(beta-hydroxyethyl)-rutosides in the venous wall, measured by laser scanning microscopy. Eur J Clin Pharmacol. 1992;43(4):423–426. doi: 10.1007/BF02220620. [DOI] [PubMed] [Google Scholar]
  18. Nocker W., Diebschlag W., Lehmacher W. 3monatige, randomisierte doppelblinde Dosis-Wirkungsstudie mit 0-(Beta-Hydroxyäthyl)-rutosid-Trinklösungen. Vasa. 1989;18(3):235–238. [PubMed] [Google Scholar]
  19. Pulvertaft T. B. General practice treatment of symptoms of venous insufficiency with oxerutins. Results of a 660 patient multicentre study in the UK. Vasa. 1983;12(4):373–376. [PubMed] [Google Scholar]
  20. Roztocil K., Fischer A., Novák P., Rázgová L. The effect of O-( -hydroxyethyl)-rutosides (HR) on the peripheral circulation in patients with chronic venous insufficiency. Eur J Clin Pharmacol. 1971 Sep;3(4):243–246. doi: 10.1007/BF00565014. [DOI] [PubMed] [Google Scholar]
  21. Roztocil K., Prerovský I., Oliva I. The effect of hydroxyethylrutosides on capillary filtration rate in the lower limb of man. Eur J Clin Pharmacol. 1977 Jul 19;11(6):435–438. doi: 10.1007/BF00562934. [DOI] [PubMed] [Google Scholar]
  22. Schneeberger E. E., Hamelin M. Interaction of serum proteins with lung endothelial glycocalyx: its effect on endothelial permeability. Am J Physiol. 1984 Aug;247(2 Pt 2):H206–H217. doi: 10.1152/ajpheart.1984.247.2.H206. [DOI] [PubMed] [Google Scholar]
  23. Schnitzer J. E., Carley W. W., Palade G. E. Albumin interacts specifically with a 60-kDa microvascular endothelial glycoprotein. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6773–6777. doi: 10.1073/pnas.85.18.6773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Swayne G. T., Smaje L. H. Dynamic compliance of single perfused frog mesenteric capillaries and rat venules: a filtration coefficient correction. Int J Microcirc Clin Exp. 1989 Feb;8(1):43–52. [PubMed] [Google Scholar]
  25. Wadworth A. N., Faulds D. Hydroxyethylrutosides. A review of its pharmacology, and therapeutic efficacy in venous insufficiency and related disorders. Drugs. 1992 Dec;44(6):1013–1032. doi: 10.2165/00003495-199244060-00009. [DOI] [PubMed] [Google Scholar]
  26. Young A. A., Gedulin B., Wolfe-Lopez D., Greene H. E., Rink T. J., Cooper G. J. Amylin and insulin in rat soleus muscle: dose responses for cosecreted noncompetitive antagonists. Am J Physiol. 1992 Aug;263(2 Pt 1):E274–E281. doi: 10.1152/ajpendo.1992.263.2.E274. [DOI] [PubMed] [Google Scholar]
  27. de Jongste A. B., Jonker J. J., Huisman M. V., ten Cate J. W., Azar A. J. A double blind three center clinical trial on the short-term efficacy of 0-(beta-hydroxyethyl)-rutosides in patients with post-thrombotic syndrome. Thromb Haemost. 1989 Nov 24;62(3):826–829. [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES