Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Jun;154(3):1440–1445. doi: 10.1128/jb.154.3.1440-1445.1983

Hybridization of cloned Rhodopseudomonas capsulata photosynthesis genes with DNA from other photosynthetic bacteria.

J T Beatty, S N Cohen
PMCID: PMC217621  PMID: 6406432

Abstract

The homology of Rhodopseudomonas capsulata DNA segments carrying photosynthesis genes with sequences present in total DNA from certain other photosynthetic and non-photosynthetic bacterial species was determined by hybridization. R. capsulata DNA fragments that carry loci for production of peptide components of the photosynthetic reaction center and light-harvesting I antenna complex were found to hybridize to DNA from some photosynthetic species. However, fragments that carry carotenoid or bacteriochlorophyll biosynthesis genes showed either weak or undetectable heterospecific hybridization under the conditions employed.

Full text

PDF
1440

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Parker B. A., Reiser J., Renart J., Stark G. R., Wahl G. M. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol. 1979;68:220–242. doi: 10.1016/0076-6879(79)68017-5. [DOI] [PubMed] [Google Scholar]
  2. Ambler R. P., Bartsch R. G., Daniel M., Kamen M. D., McLellan L., Meyer T. E., Van Beeumen J. Amino acid sequences of bacterial cytochromes c' and c-556. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6854–6857. doi: 10.1073/pnas.78.11.6854. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brenner D. J., Fanning G. R., Johnson K. E., Citarella R. V., Falkow S. Polynucleotide sequence relationships among members of Enterobacteriaceae. J Bacteriol. 1969 May;98(2):637–650. doi: 10.1128/jb.98.2.637-650.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Busslinger M., Rusconi S., Birnstiel M. L. An unusual evolutionary behaviour of a sea urchin histone gene cluster. EMBO J. 1982;1(1):27–33. doi: 10.1002/j.1460-2075.1982.tb01119.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Campbell A. Evolutionary significance of accessory DNA elements in bacteria. Annu Rev Microbiol. 1981;35:55–83. doi: 10.1146/annurev.mi.35.100181.000415. [DOI] [PubMed] [Google Scholar]
  6. Denney R. M., Yanofsky C. Detection of tryptophan messenger RNA in several bacterial species and examination of the properties of heterologous DNA-RNA hybrids. J Mol Biol. 1972 Mar 14;64(2):319–339. doi: 10.1016/0022-2836(72)90501-3. [DOI] [PubMed] [Google Scholar]
  7. Dickerson R. E. Evolution and gene transfer in purple photosynthetic bacteria. Nature. 1980 Jan 10;283(5743):210–212. doi: 10.1038/283210a0. [DOI] [PubMed] [Google Scholar]
  8. Doolittle R. F. Similar amino acid sequences: chance or common ancestry? Science. 1981 Oct 9;214(4517):149–159. doi: 10.1126/science.7280687. [DOI] [PubMed] [Google Scholar]
  9. Filer D., Furano A. V. Portions of the gene encoding elongation factor Tu are highly conserved in prokaryotes. J Biol Chem. 1980 Jan 25;255(2):728–734. [PubMed] [Google Scholar]
  10. GOODMAN H. M., RICH A. Formation of a DNA-soluble RNA hybrid and its relation to the origin, evolution, and degeneracy of soluble RNA. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2101–2109. doi: 10.1073/pnas.48.12.2101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hyman R. W., Brunovskis I., Summers W. C. DNA base sequence homology between coliphages T7 and phiII and between T3 and phiII as determined by heteroduplex mapping in the electron microscope. J Mol Biol. 1973 Jun 25;77(2):189–196. doi: 10.1016/0022-2836(73)90330-6. [DOI] [PubMed] [Google Scholar]
  12. Marrs B. Mobilization of the genes for photosynthesis from Rhodopseudomonas capsulata by a promiscuous plasmid. J Bacteriol. 1981 Jun;146(3):1003–1012. doi: 10.1128/jb.146.3.1003-1012.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mazur B. J., Rice D., Haselkorn R. Identification of blue-green algal nitrogen fixation genes by using heterologous DNA hybridization probes. Proc Natl Acad Sci U S A. 1980 Jan;77(1):186–190. doi: 10.1073/pnas.77.1.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moore R. L., McCarthy B. J. Comparative study of ribosomal ribonucleic acid cistrons in enterobacteria and myxobacteria. J Bacteriol. 1967 Oct;94(4):1066–1074. doi: 10.1128/jb.94.4.1066-1074.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Packham N. K., Tiede D. M., Mueller P., Dutton P. L. Construction of a flash-activated cyclic electron transport system by using bacterial reaction centers and the ubiquinone-cytochrome b-c1/c segment of mitochondria. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6339–6343. doi: 10.1073/pnas.77.11.6339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pemberton J. M., Bowen A. R. High-frequency chromosome transfer in Rhodopseudomonas sphaeroides promoted by broad-host-range plasmid RP1 carrying mercury transposon Tn501. J Bacteriol. 1981 Jul;147(1):110–117. doi: 10.1128/jb.147.1.110-117.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Riley M., Anilionis A. Conservation and variation of nucleotide sequences within related bacterial genomes: enterobacteria. J Bacteriol. 1980 Jul;143(1):366–376. doi: 10.1128/jb.143.1.366-376.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ruvkun G. B., Ausubel F. M. Interspecies homology of nitrogenase genes. Proc Natl Acad Sci U S A. 1980 Jan;77(1):191–195. doi: 10.1073/pnas.77.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schopf J. W. The evolution of the earliest cells. Sci Am. 1978 Sep;239(3):110-2, 114, 116-20 passim. doi: 10.1038/scientificamerican0978-110. [DOI] [PubMed] [Google Scholar]
  20. Schottel J. L., Bibb M. J., Cohen S. N. Cloning and expression in streptomyces lividans of antibiotic resistance genes derived from Escherichia coli. J Bacteriol. 1981 Apr;146(1):360–368. doi: 10.1128/jb.146.1.360-368.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  22. Taylor D. P., Cohen S. N., Clark W. G., Marrs B. L. Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique. J Bacteriol. 1983 May;154(2):580–590. doi: 10.1128/jb.154.2.580-590.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Woese C. R., Gibson J., Fox G. E. Do genealogical patterns in purple photosynthetic bacteria reflect interspecific gene transfer? Nature. 1980 Jan 10;283(5743):212–214. doi: 10.1038/283212a0. [DOI] [PubMed] [Google Scholar]
  24. de Bont J. A., Scholten A., Hansen T. A. Dna-Dna hybridization of Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides and Rhodopseudomonas sulfidophila strains. Arch Microbiol. 1981 Jan;128(3):271–274. doi: 10.1007/BF00422528. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES