Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Jun;154(3):1472–1475. doi: 10.1128/jb.154.3.1472-1475.1983

Incorporation of radioactivity from amino acids in the presence of cycloheximide demonstrates gluconeogenic activity in Mucor rouxii.

S Said, H F Terenzi
PMCID: PMC217628  PMID: 6133846

Abstract

Mucor rouxii cells induced for gluconeogenesis incorporated radioactivity from [14C]glutamic acid into trichloroacetic acid-precipitable material in the presence of 200 micrograms of cycloheximide per ml. This metabolic capacity was repressed by hexoses and required amino acids for induction. These results suggest that the incorporation of amino acids in the presence of cycloheximide represents gluconeogenic activity with associated polysaccharide synthesis.

Full text

PDF
1472

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTNICKI-GARCIA S., NICKERSON W. J. Nutrition, growth, and morphogenesis of Mucor rouxii. J Bacteriol. 1962 Oct;84:841–858. doi: 10.1128/jb.84.4.841-858.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Becker J. U. A method for glycogen determination in whole yeast cells. Anal Biochem. 1978 May;86(1):56–64. doi: 10.1016/0003-2697(78)90318-4. [DOI] [PubMed] [Google Scholar]
  3. Dow J. M., Villa V. D. Oligoglucuronide production in Mucor rouxii: evidence for a role for endohydrolases in hyphal extension. J Bacteriol. 1980 Jun;142(3):939–944. doi: 10.1128/jb.142.3.939-944.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Elorza M. V., Sentandreu R. Effect of cycloheximide on yeast cell wall synthesis. Biochem Biophys Res Commun. 1969 Aug 22;36(5):741–747. doi: 10.1016/0006-291x(69)90672-x. [DOI] [PubMed] [Google Scholar]
  5. Haidle C. W., Storck R. Control of dimorphism in Mucor rouxii. J Bacteriol. 1966 Oct;92(4):1236–1244. doi: 10.1128/jb.92.4.1236-1244.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Haidle C. W., Storck R. Inhibition by cycloheximide of protein and RNA synthesis in Mucor rouxii. Biochem Biophys Res Commun. 1966 Jan 24;22(2):175–180. doi: 10.1016/0006-291x(66)90428-1. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Sternlicht E., Katz D., Rosenberger R. F. Subapical wall synthesis and wall thickening induced by cycloheximide in hyphae of Aspergillus nidulans. J Bacteriol. 1973 May;114(2):819–823. doi: 10.1128/jb.114.2.819-823.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES