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. 1983 Jun;154(3):1476–1479. doi: 10.1128/jb.154.3.1476-1479.1983

Nonsense mutations in the can1 locus of Saccharomyces cerevisiae.

B I Ono, Y Ishino, S Shinoda
PMCID: PMC217629  PMID: 6343355

Abstract

Yeast mutants resistant to L-canavanine were selected. All were recessive and fell into the can1 complementation group. Nonsense mutations were identified among them by using a set of different suppressors. Frequencies of UAA, UAG, and presumed UGA mutations were 14.8, 0.8, and 0.4%, respectively. A high incidence of nonsense mutations having discriminatory suppression patterns was characteristic of the locus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chattoo B. B., Palmer E., Ono B., Sherman F. Patterns of Genetic and Phenotypic Suppression of lys2 Mutations in the Yeast SACCHAROMYCES CEREVISIAE. Genetics. 1979 Sep;93(1):67–79. doi: 10.1093/genetics/93.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Denis-Duphil M., Lacroute F. Fine structure of the ura 2 locus in Saccharomyces cervisiae. I. In vivo complementation studies. Mol Gen Genet. 1971;112(4):354–364. doi: 10.1007/BF00334436. [DOI] [PubMed] [Google Scholar]
  3. Gilmore R. A., Stewart J. W., Sherman F. Amino acid replacements resulting from super-suppression of nonsense mutants of iso-1-cytochrome c from yeast. J Mol Biol. 1971 Oct 14;61(1):157–173. doi: 10.1016/0022-2836(71)90213-0. [DOI] [PubMed] [Google Scholar]
  4. Grenson M., Mousset M., Wiame J. M., Bechet J. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system. Biochim Biophys Acta. 1966 Oct 31;127(2):325–338. doi: 10.1016/0304-4165(66)90387-4. [DOI] [PubMed] [Google Scholar]
  5. Hawthorne D. C., Leupold U. Suppressors in yeast. Curr Top Microbiol Immunol. 1974;64(0):1–47. doi: 10.1007/978-3-642-65848-8_1. [DOI] [PubMed] [Google Scholar]
  6. Hawthorne D. C. UGA mutations and UGA suppressors in yeast. Biochimie. 1976;58(1-2):179–182. doi: 10.1016/s0300-9084(76)80368-9. [DOI] [PubMed] [Google Scholar]
  7. Korch C. T., Snow R. Allelic Complementation in the First Gene for Histidine Biosynthesis in SACCHAROMYCES CEREVISIAE. I. Characteristics of Mutants and Genetic Mapping of Alleles. Genetics. 1973 Jun;74(2):287–305. doi: 10.1093/genetics/74.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Liebman S. W., Sherman F., Stewart J. W. Isolation and characterization of amber suppressors in yeast. Genetics. 1976 Feb;82(2):251–272. doi: 10.1093/genetics/82.2.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Liebman S. W., Stewart J. W., Parker J. H., Sherman F. Leucine insertion caused by a yeast amber suppressor. J Mol Biol. 1977 Jan 5;109(1):13–22. doi: 10.1016/s0022-2836(77)80043-0. [DOI] [PubMed] [Google Scholar]
  10. Mortimer R. K., Hawthorne D. C. Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes. Genetics. 1973 May;74(1):33–54. doi: 10.1093/genetics/74.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ono B. I., Stewart J. W., Sherman F. Serine insertion caused by the ribosomal suppressor SUP46 in yeast. J Mol Biol. 1981 Apr 15;147(3):373–379. doi: 10.1016/0022-2836(81)90489-7. [DOI] [PubMed] [Google Scholar]
  12. Ono B. I., Stewart J. W., Sherman F. Yeast UAA suppressors effective in psi+ strains serine-inserting suppressors. J Mol Biol. 1979 Feb 15;128(1):81–100. doi: 10.1016/0022-2836(79)90309-7. [DOI] [PubMed] [Google Scholar]
  13. Ono B. I., Stewart J. W., Sherman F. Yeast UAA suppressors effective in psi+ strains: leucine-inserting suppressors. J Mol Biol. 1979 Aug 15;132(3):507–520. doi: 10.1016/0022-2836(79)90272-9. [DOI] [PubMed] [Google Scholar]
  14. Ono B. I., Wills N., Stewart J. W., Gesteland R. F., Sherman F. Serine-inserting UAA suppression mediated by yeast tRNASer. J Mol Biol. 1981 Aug 15;150(3):361–373. doi: 10.1016/0022-2836(81)90552-0. [DOI] [PubMed] [Google Scholar]
  15. Rafalski A., Kohli J., Agris P., Söll D. The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe. Nucleic Acids Res. 1979 Jun 25;6(8):2683–2695. doi: 10.1093/nar/6.8.2683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sherman F., Stewart J. W., Jackson M., Gilmore R. A., Parker J. H. Mutants of yeast defective in iso-1-cytochrome c. Genetics. 1974 Jun;77(2):255–284. doi: 10.1093/genetics/77.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Singh A., Sherman F. Genetic and physiological characterization of met15 mutants of Saccharomyces cerevisiae: a selective system for forward and reverse mutations. Genetics. 1975 Sep;81(1):75–97. doi: 10.1093/genetics/81.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wetzel R., Kohli J., Altruda F., Söll D. Identification and nucleotide sequence of the sup8-e UGA-suppressor leucine tRNA from Schizosaccharomyces pombe. Mol Gen Genet. 1979 May 4;172(2):221–228. doi: 10.1007/BF00268286. [DOI] [PubMed] [Google Scholar]
  19. Whelan W. L., Gocke E., Manney T. R. The CAN1 locus of Saccharomyces cerevisiae: fine-structure analysis and forward mutation rates. Genetics. 1979 Jan;91(1):35–51. doi: 10.1093/genetics/91.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]

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