Abstract
We report experimental evidence that pULB113, an RP4::mini-Mu plasmid, mediates chromosome transfer in a strain of Erwinia carotovora subsp. chrysanthemi which does not accept the F episome. This allowed us to construct a genetic map of that strain by measuring the frequencies of cotransfer of different markers (thy, leu, pro, [his, trp], thyA, rpsL, ile).
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barth P. T., Grinter N. J., Bradley D. E. Conjugal transfer system of plasmid RP4: analysis by transposon 7 insertion. J Bacteriol. 1978 Jan;133(1):43–52. doi: 10.1128/jb.133.1.43-52.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chatterjee A. K. Acceptance by Erwinia spp. of R plasmid R68.45 and its ability to mobilize the chromosome of Erwinia chrysanthemi. J Bacteriol. 1980 Apr;142(1):111–119. doi: 10.1128/jb.142.1.111-119.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chatterjee A. K., Starr M. P. Donor strains of the soft-rot bacterium Erwinia chrysanthemi and conjugational transfer of the pectolytic capacity. J Bacteriol. 1977 Dec;132(3):862–869. doi: 10.1128/jb.132.3.862-869.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chatterjee A. K., Starr M. P. Genetic transfer of episomic elements among Erwinia species and other enterobacteria: F'Lac+. J Bacteriol. 1972 Jul;111(1):169–176. doi: 10.1128/jb.111.1.169-176.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Faelen M., Resibois A., Toussaint A. Mini-mu: an insertion element derived from temperate phage mu-1. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1169–1177. doi: 10.1101/sqb.1979.043.01.132. [DOI] [PubMed] [Google Scholar]
- Gottesman M. M., Gottesman M. E., Gottesman S., Gellert M. Characterization of bacteriophage lambda reverse as an Escherichia coli phage carrying a unique set of host-derived recombination functions. J Mol Biol. 1974 Sep 15;88(2):471–487. doi: 10.1016/0022-2836(74)90496-3. [DOI] [PubMed] [Google Scholar]
- Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kotoujansky A., Lemattre M., Boistard P. Utilization of a thermosensitive episome bearing transposon TN10 to isolate Hfr donor strains of Erwinia carotovora subsp. chrysanthemi. J Bacteriol. 1982 Apr;150(1):122–131. doi: 10.1128/jb.150.1.122-131.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Résibois A., Toussaint A., van Gijsegem F., Faelen M. Physical characterization of mini-mu and mini-D108. Gene. 1981 Jun-Jul;14(1-2):103–113. doi: 10.1016/0378-1119(81)90152-9. [DOI] [PubMed] [Google Scholar]
- STACEY K. A., SIMSON E. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:554–555. doi: 10.1128/jb.90.2.554-555.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Gijsegem F., Toussaint A. Chromosome transfer and R-prime formation by an RP4::mini-Mu derivative in Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, and Proteus mirabilis. Plasmid. 1982 Jan;7(1):30–44. doi: 10.1016/0147-619x(82)90024-5. [DOI] [PubMed] [Google Scholar]